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Capture-ELISA for serum IgM antibody to respiratory syncytial virus

Published online by Cambridge University Press:  19 October 2009

R. Cevenini ,
M. Donati ,
S. Bertini ,
A. Moroni  and
V. Sambri
R. Cevenini
Affiliation:
Institute of Microbiology, University of Bologna, Ospedale S. Orsola, via Massarenti 9-40138 Bologna, Italy
M. Donati
Affiliation:
Institute of Microbiology, University of Bologna, Ospedale S. Orsola, via Massarenti 9-40138 Bologna, Italy
S. Bertini
Affiliation:
Institute of Microbiology, University of Bologna, Ospedale S. Orsola, via Massarenti 9-40138 Bologna, Italy
A. Moroni
Affiliation:
Institute of Microbiology, University of Bologna, Ospedale S. Orsola, via Massarenti 9-40138 Bologna, Italy
V. Sambri
Affiliation:
Institute of Microbiology, University of Bologna, Ospedale S. Orsola, via Massarenti 9-40138 Bologna, Italy
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A four-component solid-phase capture enzyme immunoassay was set up to test for serum IgM antibody to respiratory syncytial (RS) virus and was compared with immunofluorescence assay (IFA).

A total of 128 young children with acute respiratory infections were studied. Thirty-six were shown to be RS virus-positive by the detection of RS virus in nasopharyngeal secretions and 92 were RS virus-negative. A serum specimen was collected after admission to the hospital (days 0–4) and a further specimen was obtained during days 10–14. Out of 36 RS virus-positive patients, 28 (77·7%) were found to be positive for IgM by both capture-ELISA and IFA. Out of 92 RS virus-negative patients 5 (5·4%) were IgM-positive. Four false-positive results were obtained by IFA due to the presence of rheumatoid factor.

The capture-ELISA was shown to be a reliable technique in detecting specific IgM antibody to RS virus.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 3 , December 1986 , pp. 511 - 517
Copyright
Copyright © Cambridge University Press 1986

References

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