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Characterization of rotavirus electropherotypes excreted by symptomatic and asymptomatic infants

Published online by Cambridge University Press:  15 May 2009

J. Fernández ,
A. M. Sandino ,
J. Pizarro ,
L. F. Avendaño ,
J. M. Pizarro  and
E. Spencer
J. Fernández
Affiliation:
Unidad de Virología, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Macul 5540, Casilla 138-11, Santiago, Chile
A. M. Sandino
Affiliation:
Unidad de Virología, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Macul 5540, Casilla 138-11, Santiago, Chile
J. Pizarro
Affiliation:
Unidad de Virología, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Macul 5540, Casilla 138-11, Santiago, Chile
L. F. Avendaño
Affiliation:
Sección de Virología, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Chile
J. M. Pizarro
Affiliation:
Unidad de Virología, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Macul 5540, Casilla 138-11, Santiago, Chile
E. Spencer*
Affiliation:
Unidad de Virología, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Macul 5540, Casilla 138-11, Santiago, Chile
*
* Author for correspondence.
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Human rotavirus isolates from 1100 stool samples were analyzed by polyacrylamide gel electrophoresis, and 48 different migration patterns were detected. Heterogeneity in the migration of segment 10 was observed in both long and short electropherotypes in which three long and two short patterns were identified. In spite of these variations all short and long electropherotypes were subgrouped by enzyme immunoassay as subgroups I and II respectively. Mixed infections were detected in 17% of cases and the subgrouping correlated with the corresponding electropherotypes. The same electropherotypes were present in severe, mild and asymptomatic cases and no electropherotype was particularly associated with greater virulence. Furthermore, the electropherotypes isolated from nosocomial asymptomatic cases were the same as those detected from those admitted with severe diarrheoa. It seems unlikely that electropherotyping can be used to identify more virulent strains of rotavirus.

Type
Research Article
Information
Epidemiology & Infection , Volume 106 , Issue 1 , February 1991 , pp. 189 - 198
Copyright
Copyright © Cambridge University Press 1991

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