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Distribution of mip-related sequences in 39 species (48 serogroups) of Legionellaceae

Published online by Cambridge University Press:  15 May 2009

S. Riffard ,
F. Vandenesch ,
M. Reyrolle  and
J. Etienne
S. Riffard
Affiliation:
UPRES EA1655, Faculté de Médecine Rene Laennec, rue Guillaume Paradin, 69372 Lyon Cedex 08, France
F. Vandenesch
Affiliation:
UPRES EA1655, Faculté de Médecine Rene Laennec, rue Guillaume Paradin, 69372 Lyon Cedex 08, France
M. Reyrolle
Affiliation:
Centre National de Référence des Legionella, Laboratoire de Bactériologie, Hôpital Edouard Herriot, Place d'Arsonval, 69437 Lyon Cedex 03, France
J. Etienne*
Affiliation:
UPRES EA1655, Faculté de Médecine Rene Laennec, rue Guillaume Paradin, 69372 Lyon Cedex 08, France Centre National de Référence des Legionella, Laboratoire de Bactériologie, Hôpital Edouard Herriot, Place d'Arsonval, 69437 Lyon Cedex 03, France
*
* Author for correspondence: J. Etienne, UPRES EA1655, Faculte de Medecine Rene Laennec, rue Guillaume Paradin, 69372 Lyon Cedex 08, France.
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The macrophage infectivity potentiator gene (mip) from Legionella pneumophila is a major virulence factor of the species. Thus, mip-detection by amplification has been proposed to assess the presence of L. pneumophila in clinical and environmental samples. The distribution of mip-related sequences within the Legionellaceae was studied by DNA amplification using mip-specific primers followed by Southern blot hybridization with an internal probe. Thirty nine species (48 serogroups) of Legionellaceae were screened in this attempt. Using this approach, sequences related to mip were observed in 89% of the tested species including the most recently described L. fairfieldensis, L. lansingensis and L. shakespearei. In several cases, cloning and sequencing of the amplified products confirmed the high levels of similarity between the sequence found in non-pneumophila species with that of the L. pneumophila mip gene. This confirms previous reports that mip related genes are widespread among Legionellaceae and therefore specific detection of the species L. pneumophila cannot be based on mip-targeted amplification.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 3 , December 1996 , pp. 501 - 506
Copyright
Copyright © Cambridge University Press 1996

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