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DNA restriction digest and ribosomal RNA gene patterns of Campylobacter jejuni: a comparison with bio-, sero-, and bacteriophage-types of United Kingdom outbreak strains

  • R. J. Owen (a1), J. Hernandez (a1) and F. Bolton (a2)

Summary

DNA restriction endonuclease (Hae III and Hind III) total digest and 16S and 23S ribosomal (r)RNA gene patterns (ribopatterns) were determined for 18 isolates of Campylobacter jejuni from three separate outbreaks of diarrhoea in the north of England. Strains were also characterized by biotyping, serotyping and phage typing. Comparisons of the DNA patterns by visual and numerical methods revealed five distinct strain groupings with clear differences between isolates from different outbreaks as well as some heterogeneity between strains within the community outbreak and one of the school outbreaks. An excellent correlation was observed between the genomic DNA fingerprints data and the Preston bacteriophage group, both of which gave better discrimination than biotyping and serotyping alone or in combination. Only one phage group (PG 37) was not confirmed by the DNA data. DNA fingerprints therefore provide additional information of value in studying the epidemiology of outbreaks of C. jejuni.

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References

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1.Skirrow, MB. A demographic survey of Campylobacter, Salmonella and Shigella infections in England. A Public Health Laboratory Service Survey. Epidemiol Infect 1987; 49: 647–57.
2.Humphrey, TJ. Salmonella, Campylobacter and poultry: possible control measures. Abstr Hyg Comm Dis 1989; 64: R1–R8.
3.Skirrow, MB. Campylobacter perspectives. PHLS Microbiol Digest 1989; 6: 113–7.
4.Blaser, MJ, Taylor, DN, Feldman, RA. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev 1983; 5: 157–76.
5.Franco, DA. Campylobacter species: considerations for controlling a foodborne pathogen. J Fd Protect 1988; 51: 145–53.
6.Tauxe, RV, Hargrett-Bean, N, Patton, CM, Wachsmuth, IK. Campylobacter isolates in the United States. 1982–1986. MMWR 1989; 37: SS–2, 113.
7.Lior, H. New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and ‘Campylobacter laridis. J Clin Microbiol 1984; 20: 636–40.
8.Bolton, FJ, Holt, AV, Hutchinson, DN. Campylobacter biotyping scheme of epidemiological value. J Clin Pathol 1984; 37: 677–81.
9.Penner, JL, Hennessy, JN. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol 1980; 12: 732–7.
10.Lior, H, Woodward, DL, Edgar, JA, LaRoche, LJ, Gill, P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol 1982; 15: 761–8.
11.Hébert, GA, Hollis, DG, Weaver, RE, Steigerwalt, AG, McKinney, RM, Brenner, DJ. Serogroups of Campylobacter jejuni. Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence. J Clin Microbiol 1983; 17: 529–38.
12.Tenover, FC, Knapp, JS, Patton, C, Plurde, JJ. Use of auxotyping for epidermiological studies of Campylobacter jejuni and Campylobacter coli. Infect Immun 1985; 48: 384–8.
13.Grajewski, BA, Kusek, JW, Gelfand, HM. Development of a bacteriophage typing system for Campylobacter jejuni and Campylobacter coli. J Clin Microbiol 1985; 22: 13–8.
14.Bradbury, WC, Pearson, AD, Marko, MA, Congi, RV, Penner, JL. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol 1984; 19: 342–6.
15.Owen, RJ, Beck, A, Borman, P. Restriction endonuclease digest patterns of chromosomal DNA from nitrate-negative Campylobacter jejuni-like organisms. Euro J Clin Microbiol 1985; 1: 281–7.
16.Penner, JL, Hennessy, JN, Mills, SD, Bradbury, WC. Application of serotyping and chromosomal restriction endonuclease digest analysis in investigating a laboratory acquired case of Campylobacter jejuni enteritis. J Clin Microbiol 1983; 18: 1427–8.
17.Jones, DM, Sutcliffe, EM, Abbott, JD. Serotyping of Campylobacter species by combined use of two methods. Eur J Clin Microbiol 1985; 4: 562–5.
18.Lior, H, Woodward, D, Khaklvia, R. Characterization of Campylobacter jejuni outbreaks by 3 epidemiological typing markers. In: Kaijser, B, Falsen, E. eds. Campylobacter IV. Kungalv: Goterna. 1987; 103–4.
19.Owen, RJ. Chromosomal DNA fingerprinting–a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol 1989; 30: 8999.
20.Salama, SM, Bolton, FJ, Hutchinson, DN. Application of a new phage typing scheme to campylobacters isolated during outbreaks. J Epidemiol Infect 1990. In press.
21.Mill, I, Bolton, FJ, Dawkins, HC. An outbreak of Campylobacter enteritis transmitted by puppies. Environ Hlth 1987; 95: 11–4.
22.Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.
23.Pitcher, DG, Owen, RJ, Dyal, P, Beck, A. Synthesis of a biotinylated DNA probe to detect ribosomal RNA cistrons in Providencia stuartii. FEMS Microbiol Lett 1987; 48: 283–7.
24.Maniatis, T, Fritsch, EF, Sambrook, J. Molecular cloning: a laboratory manual. Cold Spring Harbor NY: Cold Spring Harbor Laboratory, 1982.
25.Sneath, PHA, Sokal, RR. Numerical taxonomy. San Francisco: WH Freeman Co, 1973.
26.Owen, RJ, Beck, A. Evaluation of three procedures using a laser densitometer and microcomputer for estimating molecular sizes of restriction endonuclease digest fragments and application to Campylobacter jejuni chromosomal DNA. Lett Appl Microbiol 1987; 4: 58.
27.Preston, MA, Penner, JL. Characterization of cross-reacting serotypes of Campylobacter jejuni. Canad J Microbiol 1989; 35: 265–73.
28.Romaniuk, PJ, Trust, TJ. Identification of Campylobacter species by Southern hybridization of genomic DNA using an oligonucleotide probe for 16S rRNA genes. FEMS Microbiol Lett 1987; 43: 331–5.
29.Owen, RJ, Costas, M, Dawson, C. Application of different chromosomal DNA restriction digest fingerprints to specific and subspecific identification of Campylobacter isolates. J Clin Microbiol 1989; 27: 2338–43.

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