Hostname: page-component-848d4c4894-m9kch Total loading time: 0 Render date: 2024-05-22T08:05:22.218Z Has data issue: false hasContentIssue false

Genetic analysis of antibiotic-resistance determinants in multidrug-resistant Shigella strains isolated from Chilean children

Published online by Cambridge University Press:  06 October 2004

C. S. TORO
Affiliation:
Laboratorio de Antibióticos, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
M. FARFÁN
Affiliation:
Laboratorio de Antibióticos, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
I. CONTRERAS
Affiliation:
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile
O. FLORES
Affiliation:
Laboratorio de Antibióticos, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
N. NAVARRO
Affiliation:
Laboratorio de Antibióticos, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
G. C. MORA
Affiliation:
Laboratorio de Microbiología, Departamento de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile
V. PRADO
Affiliation:
Laboratorio de Antibióticos, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A total of 162 clinical isolates of Shigella collected from children in a semi-rural community of Chile were examined for the presence of genetic determinants of resistance to ampicillin, chloramphenicol, tetracycline, and trimethoprim. Ampicillin resistance was most frequently associated with the presence of blaOXA in S. flexneri and with blaTEM in S. sonnei. The blaOXA gene but not blaTEM was located in class 1 integrons. The dhfrIa gene encoding for resistance to trimethoprim was associated to class 2 integrons and detected exclusively in S. flexneri, whereas dhfrIIIc was found in all S. sonnei strains and in 10% of the S. flexneri isolates. Cat, coding for choramphenicol resistance, and blaOXA genes were located in the chromosome in all cases, whereas tetA gene, coding for tetracycline resistance, and blaTEM, dhfrIa and dhfrIIIc genes were found either in the chromosome or in conjugative plasmids. Our results show a heterogenous distribution of antibiotic-resistance determinants between S. flexneri and S. sonnei.

Type
Research Article
Copyright
2004 Cambridge University Press