Skip to main content Accessibility help
×
Home
Hostname: page-component-56f9d74cfd-fpcrz Total loading time: 1.118 Render date: 2022-06-26T08:44:46.160Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "useNewApi": true }

Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA

Published online by Cambridge University Press:  01 April 1997

R. BORROW
Affiliation:
Manchester Public Health Laboratory, Withington Hospital, Nell Lane, Manchester M20 2LR, UK
H. CLAUS
Affiliation:
Medizinische Hochschule Hannover, Institut für Medizinische Mikrobiologie, Hannover 30623, Germany
M. GUIVER
Affiliation:
Manchester Public Health Laboratory, Withington Hospital, Nell Lane, Manchester M20 2LR, UK
L. SMART
Affiliation:
SMPRL, Ruchill Hospital, Glasgow G20 9LB, UK
D. M. JONES
Affiliation:
Manchester Public Health Laboratory, Withington Hospital, Nell Lane, Manchester M20 2LR, UK
E. B. KACZMARSKI
Affiliation:
Manchester Public Health Laboratory, Withington Hospital, Nell Lane, Manchester M20 2LR, UK
M. FROSCH
Affiliation:
Medizinische Hochschule Hannover, Institut für Medizinische Mikrobiologie, Hannover 30623, Germany
A. J. FOX
Affiliation:
Manchester Public Health Laboratory, Withington Hospital, Nell Lane, Manchester M20 2LR, UK
Rights & Permissions[Opens in a new window]

Abstract

HTML view is not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Rapid, non-culture, serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. The impending availability of polysaccharide–protein conjugate vaccines for serogroup C disease requires maximal case ascertainment, with serogroup determination, at a time when the number of culture confirmed meningococcal infections is decreasing. A polymerase chain reaction assay (PCR), based on a restriction fragment length polymorphism (RFLP) in the meningococcal serogroup B and C sialyltransferase (siaD) gene, was developed to combine the non-culture diagnosis of meningococcal infection from CSF, whole blood and serum with serogroup (B and C) identification. The PCR assay was adapted to an ELISA format incorporating hybridization with serogroup-specific B and C oligonucleotide probes. Specificity for CSFs was 100% and sensitivities were respectively 81, 63 and 30% for CSFs, whole blood and sera. The serogroup-specific PCR ELISA is a significant addition to currently available tests for non-culture diagnosis of meningococcal infection and outbreak investigation.

Type
Research Article
Copyright
© 1997 Cambridge University Press
You have Access
92
Cited by

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *