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Observations on experimental inactivated vaccines for contagious bovine pleuropneumonia

Published online by Cambridge University Press:  19 October 2009

M. A. Gray ,
P. Simam  and
G. R. Smith
M. A. Gray
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, PO Box 32, Kikuyu, Kenya
P. Simam
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute, PO Box 32, Kikuyu, Kenya
G. R. Smith
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, Regent's Park, London NW1 4RY
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Summary

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In two trials the efficacy of inactivated vaccines against contagious bovine pleuropneumonia was tested by exposing vaccinated cattle to droplet infection provided by close contact with experimentally infected ‘donors’. Complete protection was given by an extreme form of vaccination in which a heavy suspension of killed Mycoplasma mycoides subsp. mycoides emulsified with Freund's complete adjuvant was given in two large doses. ‘Mouse-protective antibody’ (MPA) was also produced, i.e. serum transferred to mice 2–4 h before intraperitoneal challenge prevented the development of mycoplasmaemia. However, the study did not answer the question ‘Is MPA protective for cattle?’. No protection was given by a milder form of vaccination in which a lighter suspension of killed Hiycoplasmas emulsified with Freund's incomplete adjuvant was given in a comparatively small dose on a single occasion.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 2 , October 1986 , pp. 305 - 315
Copyright
Copyright © Cambridge University Press 1986

References

Brown, R. D., Gourlay, R. N. & Macleod, A. K. (1965). The production of T1 broth culture contagious bovine pleuropneumonia vaccine. Bulletin of the Epizootic Diseases of Africa 13, 149–155.Google Scholar
Campbell, A. D. & Turner, A. W. (1953). Studies on contagious pleuropneumonia of cattle. IV An improved complement fixation test. Australian Veterinary Journal 29, 154–163.Google Scholar
Davies, G. & Gilbert, F. R. (1969). Contagious bovine pleuropneumonia vaccination in East Africa. Bulletin of Epizootic Diseases of Africa 17, 21–26.Google ScholarPubMed
Davies, G., Masiga, W. N., Shifrine, M. & Read, W. C. S. (1968). The efficacy of T1 strain broth vaccine against contagious bovine pleuropneumonia. Veterinary Record 83, 239–244.Google Scholar
Davies, G. & Read, W. C. S. (1968). A modification of the growth-inhibition test and its use for detecting Mycoplasma mycoides var. mycoides. Journal of Hygiene 66, 319–324.Google Scholar
Davies, G. & Read, W. C. S. (1969). The use of bacteriocidal agents in the primary isolation of Mycoplasma mycoides. Journal of Comparative Pathology 79, 121–125.Google Scholar
Dyson, D. A. & Smith, G. R. (1975). Contagious bovine pleuropneumonia: mouse-protective antibody in the sera of infected and vaccinated cattle. Research in Veterinary Science 19, 8–16.Google Scholar
Gourlay, R. N. (1964). Antigenicity of Mycoplasma mycoides. 1. Examination of body fluids from cases of contagious bovine pleuropneumonia. Research in Veterinary Science 5, 473–482.Google Scholar
Hooker, J. M., Smith, G. R. & Milligan, R. A. (1980). Immune response of mice, rabbits and cattle to inactivated Mycoplasma mycoides subspecies mycoides vaccines containing adjuvants. Journal of Comparative Pathology 90, 363–372.Google Scholar
Hudson, J. R. & Turner, A. W. (1963). Contagious bovine pleuropneumonia: a comparison of the efficacy of two types of vaccine. Australian Veterinary Journal 39, 373–385.CrossRefGoogle Scholar
Smith, G. R. (1968). Factors affecting bacteriaemia in mice inoculated with Mycoplasma mycoides var. mycoides. Journal of Comparative Pathology 78, 267–274.CrossRefGoogle ScholarPubMed
Smith, G. R. (1971). Mycoplasma mycoides var. mycoides: immunity and mouse-protective antibody. Journal of Comparative Pathology 81, 267–278.Google Scholar
Turner, A. W., Campbell, A. D. & Dick, A. T. (1935). Recent work on pleuropneumonia contagiosa bourn in North Queensland. Australian Veterinary Journal 11, 63–71.Google Scholar
Watters, C. (1978). A one-step biuret assay for protein in the presence of detergent. Analytical Biochemistry 88, 695–698.Google Scholar
Wilson, G. S. & Miles, A. A. (1975). Topley and Wilson's Principles of Bacteriology, Virology and Immunity, 6th edn., pp. 1655–1656. London: Edward Arnold.Google Scholar