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Phenotypic variation amongst genotypically homogeneous Legionella pneumophila serogroup 1 isolates: implications for the investigation of outbreaks of Legionnaires' disease

Published online by Cambridge University Press:  15 May 2009

T. G. Harrison ,
N. A. Saunders ,
A. Haththotuwa ,
G. Hallas ,
R. J. Birtles  and
A. G. Taylor
T. G. Harrison*
Affiliation:
PHLS Legionella Reference Unit, Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London NW9 5HT, UK
N. A. Saunders
Affiliation:
PHLS Legionella Reference Unit, Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London NW9 5HT, UK
A. Haththotuwa
Affiliation:
PHLS Legionella Reference Unit, Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London NW9 5HT, UK
G. Hallas
Affiliation:
PHLS Legionella Reference Unit, Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London NW9 5HT, UK
R. J. Birtles
Affiliation:
PHLS Legionella Reference Unit, Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London NW9 5HT, UK
A. G. Taylor
Affiliation:
PHLS Legionella Reference Unit, Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London NW9 5HT, UK
*
Corresponding author and reprint requests: Dr T. G. Harrison, PHLS Legionella Reference Unit, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT.
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Summary

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One hundred and seventy-nine isolates of Legionella pneumophila serogroup 1, obtained from a site associated with an outbreak of Legionnaires' disease, were examined by monoclonal antibody subgrouping, restriction fragment length polymorphism typing, restriction endonuclease analysis and plasmid content. Nine distinct phenotypes were detected but at the genotypic level all strains were closely related. The data presented indicate that phenotypic variation of a single parent strain can occur within an environmental site. The implications of these findings are discussed in relation to the investigation of outbreaks of Legionnaires' disease.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 2 , April 1990 , pp. 171 - 180
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

Edelstein, PH, Nakahama, C, Tobin, JO'H, et al. Paleoepidemiologic investigation of Legionnaires' disease at Wadsworth Veterans Administration hospital by using three typing methods for comparison of legionellae from clinical and environmental sources. J Clin Microbiol 1986; 23: 1121–6.CrossRefGoogle ScholarPubMed
Joly, JR, McKinney, RM, Tobin, JO'H, Bibb, WF, Watkins, ID, Ramsay, D.Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol 1986; 23: 768–71.CrossRefGoogle ScholarPubMed
Selander, RK, McKinney, RM, Whittam, TS, et al. Genetic structure of populations of Legionella pneumophila. J Bacteriol 1985; 163: 1021–37.CrossRefGoogle ScholarPubMed
Saunders, NA, Kachwalla, N, Harrison, TG, Taylor, AG. Typing of Legionella pneumophila for epidemiological studies using DNA probes. In: Fleurette, J, Bornstein, N, Marmet, D, Surgot, M, eds. Colloque Legionella. Collection Fondation Marcel Merieux, 1987: 197–203.Google Scholar
Dennis, PJ. Isolation of legionellae from environmental specimens. In:Harrison, TGTaylor, AG eds. A laboratory manual for legionella. Chichester: J Wiley 1988: 31–44.Google Scholar
Harrison, TG, Taylor, AG, eds.A laboratory manual for legionella. Chichester: J Wiley, 1988.Google Scholar
Saunders, NA. Analysis of restriction fragment length polymorphisms (RFLPs) for epidemiological tracing of bacteria using non-radioactive probes. Focus 1989: 11: 47–9.Google Scholar
Saunders, NA, Harrison, TG, Haththotuwa, A, Kachwalla, N, Taylor, AG. A typing method for Legionella pneumophila serogroup 1 based on restriction fragment length polymorphisms. J Med Microbiol. In press.Google Scholar
Kado, CI, Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–73.CrossRefGoogle ScholarPubMed
Wadowsky, RM, Yee, RB, Mezmar, L, Wing, EJ, Dowling, JN. Hot water systems as sources of Legionella pneumophila in hospital and nonhospital plumbing fixtures. App Environ Microbiol 1982; 43: 1104–10.Google Scholar
Jarvis, B.Statistical aspects of the microbiological analysis of foods. Elsevier, 1989: 142–6.Google Scholar
Morton, S, Bartlett, CLR, Bibby, LF, Hutchinson, DN, Dryer, JV, Dennis, PJ.Outbreak of legionnaires' disease from a cooling water system in a power station. Brit J Ind Med 1986; 43: 630–5.Google Scholar
Stout, JE, Yu, V, Muraca, P.Legionnaires' disease acquired within in the homes of two patients: link to the home water supply. JAMA 1987; 257: 1215–7.CrossRefGoogle Scholar
Dondero, TJ, Rendtorff, RC, Mallison, GF, et al. An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower. N Eng J Med 1980; 302: 365–70.CrossRefGoogle ScholarPubMed
Helms, CM, Massanari, RM, Wenzel, RP, et al. Legionnaires' disease associated with a hospital water system: a five-year progress report on continuous hyperchlorination. JAMA 1988; 259: 2423–7.CrossRefGoogle ScholarPubMed
Stout, JE, Joly, J, Para, M, et al. Comparison of molecular methods for subtyping patients and epidemiologically linked environmental isolates of Legionella pneumophila. J Inf Dis 1988; 157: 486–95.CrossRefGoogle ScholarPubMed
van Ketel, RJ, ter Schegget, J, Zanen, HC.Molecular epidemiology of Legionella pneumophila serogroup 1. J Clin Microbiol 1984; 20: 362–4.CrossRefGoogle ScholarPubMed
Tompkins, LS, Troup, NJ, Woods, T, Bibb, W, McKinney, RM.Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis. J Clin Microbiol 1987; 25: 1875–80.CrossRefGoogle ScholarPubMed
Edelstein, PH, Beer, KB, DeBoynton, ED.Influence of growth temperature on virulence of Legionella pneumophila. Inf Immun 1987; 55: 2701–5.CrossRefGoogle ScholarPubMed
Colbourne, JS, Dennis, PJ, Trew, RM, Berry, C, Vesey, G.Legionella and public water supplies. Wat Sci Tech 1988; 20: 5–10.CrossRefGoogle Scholar