Skip to main content Accessibility help
×
Home
Hostname: page-component-55b6f6c457-ln9sz Total loading time: 0.228 Render date: 2021-09-28T15:27:12.157Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Sequence variation in the haemagglutinin-neuraminidase gene of human parainfluenza virus type 3 isolates in the UK

Published online by Cambridge University Press:  15 May 2009

K. R. Collins
Affiliation:
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
A. J. Easton*
Affiliation:
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
*
* Corresponding author.
Rights & Permissions[Opens in a new window]

Summary

HTML view is not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The sequence variation in a 934 base-pair region of the gene encoding the haemagglutinin-neuraminidase of five human parainfluenza virus type 3 (HPIV3) isolates was determined together with that of a prototype UK strain. All of the clinical isolates were from the Manchester area of the UK and were obtained in 1990. 1991 and 1993. The gene segment was amplified by the polymerase chain reaction using HPIVB-specific oligonucleotide primers. The nucleotide homology of the strains was high, around 99% and specific differences in the UK sequences when compared with that of the US prototype strain were identified. In addition, a number of isolate-specific differences were seen. No correlation was detected between the observed nucleotide mutations and the year of isolation, which supports the hypothesis that HPIV3 shows cocirculation of a heterogeneous population of viruses rather than varying with time in a linear fashion. However, the data suggested that geographically-defined genetic lineages of HPIV3 may exist.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1995

References

1.Glezen, W, Frank, AL, Taber, LH, Kasel, JA. Parainfluenza virus type 3: Seasonality and risk of infection and reinfection in young children. J Infect Dis 1984; 150: 851–7.CrossRefGoogle ScholarPubMed
2.Black, FL. Epidemiology of paramyxoviridae. In: Kingsbury, DW, ed. The paramyxoviruses. New York: Plenum Publishing Corporation, 1991: 509–29.CrossRefGoogle Scholar
3.Easton, AJ, Eglin, RP. Epidemiology of parainfluenza virus type 3 in England and Wales over a ten-year period. Epidemiol Infect 1989; 102: 531–5.CrossRefGoogle Scholar
4.Ray, R, Compans, RW. Monoclonal antibodies reveal extensive antigenic differences between the hemagglutinin-neuraminidase glycoproteins of human and bovine parainfluenza 3 viruses. Virology 1986: 148: 232–6.CrossRefGoogle ScholarPubMed
5.Rydbeck, R, Love, A, Orvell, C, Norrby, E. Antigenic analysis of human and bovine parainfluenza virus type 3 strains with monoclonal antibodies. J Gen Tirol 1987: 68: 2153–60.CrossRefGoogle ScholarPubMed
6.Klippmark, E, Rydbeck, R, Shibuta, H, Norrby, E. Antigenic variation of human and bovine parainfluenza virus type 3 strains. J Gen Virol 1990; 71: 1577–80.CrossRefGoogle ScholarPubMed
7.van Wyke Coelingh, KL, Winter, CC, Murphy, BR. Antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus. Virology 1985: 143: 569–82.CrossRefGoogle ScholarPubMed
8.van Wyke Coelingh, KL, Winter, CC, Murphy, BR. Nucleotide and deduced amino acid sequence of hemagglutinin-neuraminidase genes of human type 3 parainfluenza viruses isolated from 1957 to 1983. Virology 1988: 162: 137–43.CrossRefGoogle ScholarPubMed
9.van Wyke Coelingh, KL, Winter, CC, Tierney, EL, London, WT, Murphy, BR. Antibody responses of humans and nonhuman primates to individual antigenic sites of the hemagglutinin-neuraminidase and fusion glycoproteins after primary infection or reinfection with parainfluenza type 3 virus. J Virol 1990; 64. 3833–43.Google ScholarPubMed
10.Hetherington, SV, Watson, AS, Scroggs, RA, Portner, A. Human parainfluenza virus type 1 evolution combines cocirculation of strains and development of geographically restricted lineages. J Infect Dis 1994: 160: 248–52.CrossRefGoogle Scholar
11.Ling, R, Easton, AL, Pringle, CR. Sequence analysis of the 22K. SH and G genes of turkey rhinotracheitis and their intergenic regions reveals a gene order different from that of other pneumoviruses. J Gen Virol 1992: 73: 1709–15.CrossRefGoogle Scholar
12.Saiki, RK, Gelfand, DH, Stoffel, S et al. . Primer-directed enzymatic amplification of dna with a thermostable dna polymerase. Science 1989: 239: 487–97.CrossRefGoogle Scholar
13.Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning, a laboratory manual. 2nd ed.Cold Spring Harbor: Cold Spring Harbor Press. 1989.Google Scholar
14.Sanger, F, Coulsen, AR, Barrell, BG, Smith, AJH, Roe, BA. Cloning in single stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 1980; 143: 161–78.CrossRefGoogle ScholarPubMed
15.Elango, N, Coligan, JE, Jambou, RC, Venkatesan, S. Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein. J Virol 1986: 57: 481–9.Google ScholarPubMed
16.Morrison, T, Portner, A. Structure, function, and intracellular processing of the glycoproteins of paramyxoviridae. In: Kingsbury, DW. ed. The paramyxoviruses. New York: Plenum Publishing Corporation. 1991: 347–83.CrossRefGoogle Scholar
17.Jorgensen, ED, Collins, PL, Lomedico, PT. Cloning and nucleotide sequence of newcastle disease virus hemagglutinin-neuraminidase mRNA: Identification of a putative sialic acid binding site. Virology 1987; 156: 1224.CrossRefGoogle ScholarPubMed
18.Colman, PM, Hoyne, PA, Lawrence, MC. Sequence and structure alignment of paramyxovirus hemagglutinin-neuraminidase with influenza virus neuraminidase. J Virol 1993: 67: 2972–80.Google ScholarPubMed
19.Higgins, DG, Sharp, PM. CLUSTAL: A package for performing multiple sequence alignment on a microcomputer. Gene 1988; 73: 237–44.CrossRefGoogle ScholarPubMed
20.Henrickson, KJ, Savatski, LL. Genetic variation of human parainfluenza virus type 1 haemagglutinin-neuraminidase: Analysis of 12 clinical isolates. J Infect Dis 1992: 166: 9951005.CrossRefGoogle Scholar
21.Yamashita, M, Krystal, M, Fitch, WM, Palese, P. Influenza B virus evolution co-circulating lineages and comparison of evolutionary pattern with those of influenza A and influenza C viruses. Virology 1988: 163: 112–22.CrossRefGoogle Scholar
22.Buonagurio, DA, Nakada, S, Desselberger, U, Krystal, M, Paleses, P. Noncumulative sequence changes in the hemagglutinin genes of influenza C virus isolates. Virology 1985: 146: 221–32.CrossRefGoogle ScholarPubMed
23.Raymond, FL, Caton, AJ, Cox, NJ, Kendal, AP, Brownlee, GG. The antigenicity and evolution of influenza H1 hemagglutinin, from 1950–1957 and 1977–1983: 2 pathways from one gene. Virology 1986: 148: 275–87.CrossRefGoogle ScholarPubMed
24.Cane, PA, Matthews, DA, Pringle, CR. Analysis of respiratory syncitial virus strain variation in successive epidemics in one city. J Clin Microbiol 1994: 32: 14.Google Scholar
You have Access
2
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Sequence variation in the haemagglutinin-neuraminidase gene of human parainfluenza virus type 3 isolates in the UK
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Sequence variation in the haemagglutinin-neuraminidase gene of human parainfluenza virus type 3 isolates in the UK
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Sequence variation in the haemagglutinin-neuraminidase gene of human parainfluenza virus type 3 isolates in the UK
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *