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Trimethoprim resistance gene in Shigella dysenteriae 1 isolates obtained from widely scattered locations of Asia

Published online by Cambridge University Press:  15 May 2009

K. Haider ,
A. Chatkaeomorakot ,
B. A. Kay ,
K. A. Talukder ,
D. N. Taylor ,
P. Echeverria  and
D. A. Sack
K. Haider
Affiliation:
International Centre for Diarrhoeal Disease Research, Bangladesh GPO Box 128, Dhaka 1000, Bangladesh
A. Chatkaeomorakot
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
B. A. Kay
Affiliation:
International Centre for Diarrhoeal Disease Research, Bangladesh GPO Box 128, Dhaka 1000, Bangladesh
K. A. Talukder
Affiliation:
International Centre for Diarrhoeal Disease Research, Bangladesh GPO Box 128, Dhaka 1000, Bangladesh
D. N. Taylor
Affiliation:
International Centre for Diarrhoeal Disease Research, Bangladesh GPO Box 128, Dhaka 1000, Bangladesh
P. Echeverria
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
D. A. Sack
Affiliation:
International Centre for Diarrhoeal Disease Research, Bangladesh GPO Box 128, Dhaka 1000, Bangladesh
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Trimethoprim-resistance genes of Shigella dysenteriae 1 strains, isolated from a different location of six different countries of Asia over a 5-year period were characterized by using three different dihydrofolate reductase (DHFR) gene probes. The trimethoprim-resistant (TMPR) strains hybridized only with the type I DHFR gene probe by colony hybridization. None of the strains hybridized with types II and III DHFR gene probes. Southern blot experiments using plasmid DNA extracted from these resistant strains indicated that the type I DHFR genes were either on a 20 MDa plasmid or might be located on the chromosome. None of the other plasmids present in S. dyysenteriae 1 strains hybridized with the probe. This indicates that the TMP resistance in these S. dysenteriae 1 strains are mediated by type I DHFR enzyme, and there may be transposition of this type I DHFR gene occurs between the 20 MDa plasmid and the chromosome in this serotype of shigella.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 2 , April 1990 , pp. 219 - 228
Copyright
Copyright © Cambridge University Press 1990

References

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