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Type C bovine botulism outbreak due to carcass contaminated non-acidified silage

  • J. MYLLYKOSKI (a1), M. LINDSTRÖM (a1), R. KETO-TIMONEN (a1), H. SÖDERHOLM (a1), J. JAKALA (a2), H. KALLIO (a3), A. SUKURA (a3) and H. KORKEALA (a1)...
Summary
SUMMARY

The first reported bovine botulism outbreak in Finland is described. Nine out of 90 cattle on a dairy farm died after being fed non-acidified silage contaminated by animal carcasses. Type C botulinum neurotoxin gene was detected in one heifer by polymerase chain reaction (PCR) and the neurotoxin was detected by the mouse bioassay. Clostridium botulinum type C was isolated from liver samples. The isolated strain was identified with amplified fragment length polymorphism (AFLP) analysis as group III C. botulinum. To our knowledge, this is the first time that a type C bovine botulism outbreak has been diagnosed by PCR and confirmed by subsequent isolation and AFLP identification of the disease strain. The importance of the acidification process in silage production to inhibit C. botulinum toxin production in silage and thus to prevent further botulism outbreaks is emphasized. Nevertheless, preformed toxin in the carcass is not destroyed by acid.

Copyright
Corresponding author
*Author for correspondence: Dr J. Myllykoski, Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, PO Box 66, 00014University of Helsinki, Finland. (Email: jan.myllykoski@helsinki.fi)
References
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1.Whitlock RH, Williamson JL. Botulism toxicoses of cattle. Proceedings of the 32nd Annual Conference of the American Association of Bovine Practitioners 1999; 32: 4553.
2.Schocken-Iturrino RP, et al. First case of type A botulism in zebu. Veterinary Record 1990; 3: 217218.
3.Allen SD, Emery CL, Lyerly DM. Clostridium. In: Murray PReds. Manual of Clinical Microbiology, 8th edn. Washington, DC: ASM Press, 2003, pp. 835856.
4.Hielm S, et al. A high prevalence of Clostridium botulinum type E in Finnish freshwater and baltic sea sediment samples. Journal of Applied Microbiology 1998; 1: 133137.
5.Dahlenborg M, Borch E, Rådstrom P. Development of a combined selection and enrichment PCR procedure for Clostridium botulinum types B, E, and F and its use to determine prevalence in fecal samples from slaughtered pigs. Applied and Environmental Microbiology 2001; 10: 47814788.
6.Dahlenborg M, Borch E, Radstrom P. Prevalence of Clostridium botulinum types B, E and F in faecal samples from Swedish cattle. International Journal of Food Microbiology 2003; 2: 105110.
7.Notermans S, Dufrenne J, Oosterom J. Persistence of Clostridium botulinum type B on a cattle farm after an outbreak of botulism. Applied and Environmental Microbiology 1981; 1: 179183.
8.Heider LC, McClure JT, Leger ER. Presumptive diagnosis of Clostridium botulinum type D intoxication in a herd of feedlot cattle. Canadian Veteterinary Journal 2001; 42: 210212.
9.Kelch WJ, et al. Fatal Clostridium botulinum toxicosis in eleven Holstein cattle fed round bale barley haylage. Journal of Veterinary Diagnostic Investigation 2000; 5: 453455.
10.Galey FD, et al. Type C botulism in dairy cattle from feed contaminated with a dead cat. Journal of Veterinary Diagnostic Investigation 2000; 3: 204209.
11.Notermans S, Kozaki S, van Schothorst M. Toxin production by Clostridium botulinum in grass. Applied Environmental Microbiology 1979; 38: 767771.
12.Notermans S. Control in fruits and vegetables. In: Hauschild AHW, Dodds KLeds. Clostridium botulinum: Ecology and Control in Foods. NY, USA: Marcel Dekker Inc., 1993, pp. 239240.
13.Martin S. Clostridium botulinum type D intoxication in a dairy herd in Ontario. Canadian Veterinary Journal 2003; 6: 493495.
14.Advisory Committee on the Microbial Safety of Foods. Report on botulism in cattle. London, United Kingdom: United Kingdom Food Standard Agency, 2005.
15.Steinman A, et al. Quantitative analysis of levels of serum immunoglobulin G against botulinum neurotoxin type D and association with protection in natural outbreaks of cattle botulism. Clinical and Vaccine Immunology 2006; 8: 862868.
16.Gessler F, Hampe K, Böhnel H. Sensitive detection of botulinum neurotoxin types C and D with an immunoaffinity chromatographic column test. Applied Environmental Microbiology 2005; 71: 78977903.
17.Fach P, et al. Investigation of animal botulism outbreaks by PCR and standard methods. FEMS Immunology and Medical Microbiology 1996; 4: 279285.
18.Chaffer M, et al. Application of PCR for detection of Clostridium botulinum type D in bovine samples. Journal of Veterinary Medicine 2006; 1: 4547.
19.Prevot V, et al. Optimization of polymerase chain reaction for detection of Clostridium botulinum type C and D in bovine samples. Zoonoses and Public Health 2007; 8: 320327.
20.Nordic Committee on Food Analysis. Clostridium botulinum. Detection in foods, blood and other test materials. Method no. 80, 2nd edn.NCFA, Espoo, Finland, 1991.
21.Lindström M, et al. Multiplex PCR assay for detection and identification of Clostridium botulinum types A, B, E, and F in food and fecal material. Applied and Environmental Microbiology 2001; 12: 56945699.
22.Williamson JL, Rocke TE, Aiken JM. In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay. Applied and Environmental Microbiology 1999; 7: 32403243.
23.Szabo EA, Pemberton JM, Desmarchelier PM. Detection of the genes encoding botulinum neurotoxin types A to E by the polymerase chain reaction. Applied and Environmental Microbiology 1993; 9: 30113020.
24.Hyytiä E, Björkroth J, Hielm S, Korkeala H. Characterization of Clostridium botulinum groups I and II by randomly amplified polymorphic DNA analysis and repetitive element sequence-based PCR. International Journal Food Microbiology 1999; 48: 179189.
25.Keto-Timonen R, et al. Identification of Clostridium species and DNA fingerprinting of Clostridium perfringens by amplified fragment length polymorphism analysis. Journal of Clinical Microbiology 2006; 11: 40574065.
26.Nordic Committee on Food Analysis. Botulinum toxin. Detection in foods, blood and other test materials. Method no. 79, 2nd edn.NCFA, Espoo, Finland, 1991.
27.Centers for Disease Control and Prevention. Clostridium botulinum monovalent and polyvalent antitoxins. Georgia, USA: U.S. Department of Health, Education, and Welfare; 1987.
28.Lindström M, Korkeala H. Laboratory diagnostics of botulism. Clinical Microbiology Reviews 2006; 2: 298314.
29.Allison MJ, Maloy SE, Matson RR. Inactivation of Clostridium botulinum toxin by ruminal microbes from cattle and sheep. Applied and Environmental Microbiology 1976; 5: 685688.
30.Neill SD, McLoughlin MF, McIlroy SG. Type C botulism in cattle being fed ensiled poultry litter. Veterinary Record 1989; 21: 558560.
31.Segner WP, Schmidt CF, Boltz JK. Enrichment, isolation, and cultural characteristics of marine strains of Clostridium botulinum type C. Applied Microbiology 1971; 6: 10171024.
32.Virtanen AI. The A-I-V-method of preserving fresh fodder. Empire Journal of Experimental Agriculture 1933; 1: 143155.
33.Silage Fermentation and Preservation. North Dakota State University (http://www.ag.ndsu.edu/pubs/ansci/dairy/as1254w.htm). Accessed 27 November 2007.
34.Lindström M, et al. Type C botulism due to toxic feed affecting 52,000 farmed foxes and minks in finland. Journal of Clinical Microbiology 2004; 10: 47184725.
35.Huss HH. Distribution of Clostridium botulinum. Applied and Environmental Microbiology 1980; 4: 764769.
36.Myllykoski J, et al. The detection and prevalence of Clostridium botulinum in pig intestinal samples. International Journal of Food Microbiology 2006; 2: 172177.
37.Otter A, et al. Risk of botulism in cattle and sheep arising from contact with broiler litter. Veterinary Record 2006; 6: 186187.
38.Siegel LS. Destruction of botulinum toxins in food and water. In: Hauschild AHW, Dodds KLeds. Clostridium botulinum: Ecology and Control in Foods. NY, USA: Marcel Dekker Inc., 1993, pp. 323341.
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Epidemiology & Infection
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