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Association between GSTP1 gene polymorphism and serum α-GST concentrations undergoing sevoflurane anaesthesia

Published online by Cambridge University Press:  01 March 2008

C. Kaymak ,
B. Karahalil ,
N. N. Ozcan  and
D. Oztuna
C. Kaymak*
Affiliation:
Kirikkale University, Faculty of Medicine, Department of Anaesthesiology and Reanimation, Kirikkale
B. Karahalil
Affiliation:
University of Gazi, Faculty of Pharmacy, Department of Toxicology, Ankara
N. N. Ozcan
Affiliation:
Ministry of Health, Ankara Training and Research Hospital, Department of Anaesthesiology and Reanimation, Ankara
D. Oztuna
Affiliation:
University of Ankara, Faculty of Medicine, Department of Biostatistics, Ankara, Turkey
*
Correspondence to: Cetin Kaymak, Department of Anaesthesiology and Reanimation, Medical Faculty, Kirikkale University, Kirikkale 71100, Turkey. E-mail: cetinkaymak@yahoo.com; Tel: +90 3182252485; Fax: +90 3182244697
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Summary

Background and objectives

The measurement of α-glutathione-S-transferase enzyme is one of the most sensitive indicators of hepatocellular function. Variation in the glutathione-S-transferase P1 gene clusters has been intensively investigated and polymorphism has been described. The aim of the study was to assess whether an association exists between glutathione-S-transferase P1 gene polymorphism and serum α-glutathione-S-transferase concentrations for the first postoperative day in patients who underwent anaesthesia with sevoflurane.

Methods

In all, 54 unrelated patients were enrolled in this study. Anaesthesia was induced with thiopental and fentanyl. Vecuronium was used for neuromuscular relaxation before endotracheal intubation. Anaesthesia was maintained with sevoflurane in a gas mixture containing 50% nitrous oxide in oxygen. Peripheral venous blood samples to determine serum α-glutathione-S-transferase concentrations were collected before induction (T1), at the end of anaesthesia (T2) and at 24-h postoperatively (T3). Enzyme-linked immunosorbent assay (ELISA) immunoassay was used to measure α-glutathione-S-transferase levels. Genomic DNA was isolated from serum samples using a genomic DNA purification kit. In order to detect the variants of glutathione-S-transferase P1, polymerase chain reaction – restriction fragment length polymorphism analysis was employed.

Results

Early postoperative serum α-glutathione-S-transferase levels for all patients were significantly increased when compared with preanaesthetic and 24-h postoperatively (P < 0.05). Serum α-glutathione-S-transferase concentrations, in individuals with glutathione-S-transferase P1 Ile105Val genotypes (heterozygote gene), remained elevated at 24 h (P < 0.05), whereas levels in individuals with glutathione-S-transferase P1 Ile105Ile (wild gene) decreased (P > 0.05).

Conclusions

Although α-glutathione-S-transferase levels were elevated in all patients after sevoflurane anaesthesia, levels remained high at 24 h in patients with glutathione-S-transferase P1 Ile105Val genotypes compared to controls.

Keywords

GLUTATHIONE S-TRANSFERASEGSTP1 POLYMORPHISMANAESTHESIA INHALATIONALSEVOFLURANE
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 25 , Issue 3 , March 2008 , pp. 193 - 199
Copyright
Copyright © European Society of Anaesthesiology 2008

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