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RESPONSES OF TWO LETTUCE CULTIVARS TO IRON DEFICIENCY

Published online by Cambridge University Press:  18 July 2012

NAJOUA MSILINI ,
HOUNEIDA ATTIA ,
MOKDED RABHI ,
NAJOUA KARRAY ,
MOKHTAR LACHAÂL  and
ZEINEB OUERGHI
NAJOUA MSILINI*
Affiliation:
Unité de Physiologie et de Biochimie de la Tolérance au Sel chez les Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia
HOUNEIDA ATTIA
Affiliation:
Unité de Physiologie et de Biochimie de la Tolérance au Sel chez les Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia
MOKDED RABHI
Affiliation:
Laboratoire d'Adaptation des Plantes aux Stress Abiotiques, Centre de Biotechnologies de la Technopole de Borj-Cédria (CBBC), BP 901, 2050 Hammam-Lif, Tunisia
NAJOUA KARRAY
Affiliation:
Unité de Physiologie et de Biochimie de la Tolérance au Sel chez les Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia
MOKHTAR LACHAÂL
Affiliation:
Unité de Physiologie et de Biochimie de la Tolérance au Sel chez les Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia
ZEINEB OUERGHI
Affiliation:
Unité de Physiologie et de Biochimie de la Tolérance au Sel chez les Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia
*
‡Corresponding author. Email: msilininajoua@yahoo.fr
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Summary

A comparative study of two lettuce varieties (Lactuca sativa: ‘Romaine’ and ‘Vista’) was conducted to understand the effect of iron deficiency on growth, biomass allocation, chlorophyll fluorescence and root and leaf enzymatic activity. After 15 days of growth in hydroponic solution under Fe-deficient and Fe-sufficient conditions, leaf chlorophyll concentration, activities of ribulose 1.5-bisphosphate carboxylase/oxygenase (Rubisco) in leaves extract, ferric chelate reductase (FCR) and phosphoenolpyruvate carboxylase (PEPC) in root extracts were measured. We found that there were significant differences in fresh matter accumulation and pigment concentration between the varieties. Fresh weight and total leaf area and leaf number were significantly reduced under iron deficiency. There was also a significant decrease in photosynthetic pigment concentration in both varieties. In response to Fe deficiency, ‘Vista’ variety showed higher FCR and PEPC activities compared to ‘Romaine’ variety. Moreover, this increase was accompanied by an enhanced accumulation of phenolic compound in roots of ‘Vista’ variety. These findings show that ‘Romaine’ was more affected by iron deficiency than ‘Vista’.

Type
Research Article
Information
Experimental Agriculture , Volume 48 , Issue 4 , October 2012 , pp. 523 - 535
Copyright
Copyright © Cambridge University Press 2012

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