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Acridine treatment of F+ and Hfr strains of Escherichia coli K12 carrying a neomycin-kanamycin resistance determinant

Published online by Cambridge University Press:  14 April 2009

June N. Grindley ,
N. D. F. Grindley  and
E. S. Anderson
June N. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
N. D. F. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
E. S. Anderson
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
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Summary

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The F factor and the K resistance determinant form an R factor (KF) in E. coli K12. In this R factor the two plasmids are independent of each other and occupy different attachment sites in the host cell.

Treatment of an F+(K) strain with acriflavine and acridine orange yielded the following classes: F+K−; F−K+; and F−K−. The K determinant was thus curable independently of F, which supports the hypothesis of independence of the two plasmids. In Hfr(K) strains, K remains curable with acridines while F is immune. Thus, K remains independent of F in both the F+ and Hfr states.

The use of higher concentrations of acridine orange than those needed for cure of K in the F+(K) strain resulted in no elimination of K, although the F factor was absent from all surviving lines tested. It is suggested that this is caused by equal inhibition of replication of both the chromosome and K, and greater inhibition of that of F.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 3 , June 1970 , pp. 327 - 334
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Anderson, E. S. (1965). A rapid screening test for transfer factors in drug-sensitive Enterobacteriaceae. Nature, London 208, 1016–1017.CrossRefGoogle ScholarPubMed
Anderson, E. S. (1966). Influence of the Δ transfer factor on the phage sensitivity of Salmonellae. Nature, London 212, 795–799.CrossRefGoogle ScholarPubMed
Anderson, E. S. (1969). The ecology and epidemiology of transferable drug resistance. In Ciba Foundation Symposium on Bacterial Episomes and Plasmids, pp. 102–115.CrossRefGoogle Scholar
Anderson, E. S. & Lewis, M. J. (1965). Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature, London 208, 843–849.Google Scholar
Anderson, E. S., Mayhew, J. N. & Grindley, N. D. F. (1969). Transfer of a neomycin-kanamycin resistance determinant by the F factor of Escherichia coli K-12. Nature, London 222, 349–351.Google Scholar
Anderson, E. S., Pitton, J.-S. & Mayhew, J. N. (1968). Restriction of bacteriophage multiplication by resistance determinants in Salmonellae. Nature, London 219, 640–641.CrossRefGoogle ScholarPubMed
Berg, C. M. & Curtiss, R. (1967). Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics 56, 503–525.Google Scholar
Cuzin, F. (1965). Un bactériophage spécifique du type sexuel F–. Comptes rendus hebdomadaires des séances de L'Académie des Sciences, Paris 260, 6482–6485.Google Scholar
Dettori, R., Maccacaro, G. A. & Piccinin, G. L. (1961). Sex specific bacteriophages of Escherichia coli K-12. Giornale di Microbiologia 9, 141–150.Google Scholar
Hirota, Y. (1960). The effect of acridine dyes on mating type factors in Escherichia coli. Proceedings of the National Academy of Sciences, Washington 46, 57–64.CrossRefGoogle ScholarPubMed
Jacob, F., Brenner, S. & Cuzin, F. (1963). On the regulation of DNA replication in bacteria. Cold Spring Harbor Symposia on Quantitative Biology 28, 329–348.CrossRefGoogle Scholar
Nagata, T. (1963). The molecular synchrony and sequential replication of DNA in Escherichia coli. Proceedings of the National Academy of Sciences, Washington 49, 551–559.CrossRefGoogle ScholarPubMed
Scaife, J. & Gross, J. D. (1962). Inhibition of multiplication of an F-lac factor in Hfr cells of Escherichia coli K-12. Biochemical and Biophysical Research Communications 7, 403–407.CrossRefGoogle Scholar
Stouthamer, A. H., De Haan, P. G. & Bulten, E. J. (1963). Kinetics of F-curing by acridine orange in relation to the number of F-particles in Escherichia coli. Genetical Research 4, 305–317.CrossRefGoogle Scholar
Vielmetter, W., Messer, W. & Schütte, A. (1968). Growth direction and segregation of the Escherichia coli chromosome. Cold Spring Harbor Symposia on Quantitative Biology 33; 585–598.Google Scholar
Watanabe, T. & Fukasawa, T. (1961). Episome mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes. Journal of Bacteriology 81, 679–683.CrossRefGoogle ScholarPubMed