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Biochemical genetics of Neurospora nuclease II: Mutagen sensitivity and other characteristics of the nuclease mutants

Published online by Cambridge University Press:  14 April 2009

N. C. Mishra  and
A. M. Forsthoefel
N. C. Mishra
Affiliation:
Department of Biology, University of South Carolina, Columbia, SC 29208, U.S.A.
A. M. Forsthoefel
Affiliation:
Department of Biology, University of South Carolina, Columbia, SC 29208, U.S.A.
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Five new nuc mutants of Neurospora crassa were characterized for their relative sensitivities to different mutagens (UV, MNG, MMS), to mitomycin-C and to histidine; latter has been shown to inhibit the growth of certain UV sensitive mutants. These mutants were also compared for their capabilities for spontaneous mutation as determined by resistance to p-fluoro-phenylalanine. Based on these characterization, the mutants seem to belong to two groups. The first group included nuc-3 and nuc-6 which showed sensitivity to all mutagen tested and possessed capability for a very high frequency of spontaneous mutation (i.e. mutator effect). The second group included nuc-4, nuc-5 and nuc-7; these were as resistant to different mutagens as the wild type strain, but possessed an antimutator effect (i.e. the frequency of spontaneous mutation by these three mutants were at least 0·5–100 × less than the wild type strains). There was some variation in these properties of mutants belonging to the two groups. Among all the five nuc mutants, nuc-3 was characterized by extreme sensitivity to all mutagens. None of the five nuc mutants were sensitive to histidine. The properties of nuc mutants are discussed in relation to their possible role in DNA repair and recombination.

Type
Research Article
Information
Genetics Research , Volume 41 , Issue 3 , June 1983 , pp. 287 - 297
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Alberts, B. M., Barry, J., Bedinger, P., Burke, R. C., Hubner, U., Liu, C. C. & Sheridan, R. (1980). Studies on the replication mechanism with the T4 bacteriophage in vitro system. In ICN-UCLA Symposia on Molecular Cellular Biology, vol. XIX (ed. Alberts, B. M. and Fox, F.), pp. 449–473. Academic Press.Google Scholar
Bryant, D. W. & Haynes, R. H. (1978). Endonuclease from Saccharomyces cereviseae shows increased activity on ultra violet irradiated nature DNA. Molecular and General Genetics 167, 139–145.CrossRefGoogle Scholar
Chow, T. Y. K. & Fraser, M. J. (1978). Sensitivity of DNA repair deficient mutants of Neurospora to histidine and to mitomycin-C. Microbiological Bulletin 45, 4–5.Google Scholar
Clark, A. J. (1976). Recombination deficient mutants of E. coli and other bacteria. Annual Review Genetics 7, 67–86.CrossRefGoogle Scholar
Cox, B. S. & Game, J. (1974). Repair systems in Saccharomyces. Mutation Research 26, 257–264.CrossRefGoogle ScholarPubMed
Davis, R. H. & DeSerres, F. (1970). Genetic and microbiological research techniques for Neurospora crassa. In Methods in Enzymology vol. 17A (ed. Tabor, A. and Tabor, C.), pp. 79–143. New York: Academic Press.Google Scholar
Delange, A. M. & Mishra, N. C. (1981). The isolation of MMS- and histidine-sensitive mutants in Neurospora crassa. Genetics 97, 247–259.CrossRefGoogle ScholarPubMed
Delange, A. M. & Mishra, N. C. (1982). Characterization of MMS-sensitive mutants of Neurospora crassa. Mutation Research 96, 187–199.CrossRefGoogle ScholarPubMed
Fraser, M. J. (1979). Alkaline deoxyribonuclease released from Neurospora crassa mycelia: two activities not released by mutants with multiple sensitivities to mutagen. Nucleic Acids Research 6, 231–246.CrossRefGoogle ScholarPubMed
Forsthoefel, A. M. & Mishra, N. C. (1983). Biochemical genetics of Neurospora nuclease I: Isolation and characterization of nuclease (nuc) mutants. Genetical Research 41, 271–286.CrossRefGoogle ScholarPubMed
Hanawalt, P. C., Cooper, P. K., Ganesan, A. K. & Smith, C. A. (1979). DNA repair in bacteria and mammalian cells. Annual Review of Biochemistry 48, 793–836.CrossRefGoogle ScholarPubMed
Haynes, R. H., Phakash, L., Resnick, M. A., Cox, B. S., Moutachi, E. & Boyd, J. B. (1978). DNA repair in lower Eukaryotes. In DNA Repair Mechanisms (ed. Hanawalt, P. C., Friedberg, E. C. and Fox, C. F.), pp. 405–412. New York: Academic Press.CrossRefGoogle Scholar
Howard Flanders, P. (1981). Inducible repair of DNA. Scientific American 245, 72–80.CrossRefGoogle ScholarPubMed
Holloman, K. & Holliday, R. (1973). Studies on a nuclease from Uslilago maydis. I. Purification, properties and implications in recombination of the enzyme. Journal of Biological Chemistry 248, 8107–8113.CrossRefGoogle ScholarPubMed
Ishikawa, T., Toh-e, A., Uno, I. & Hasunuma, K. (1969). Isolation and Characterization of nuclease mutants in Neurospora crassa. Genetics 63, 75–92.CrossRefGoogle ScholarPubMed
Kafer, E. (1981). Mutagen sensitivities and mutator effects of MMS-sensitive mutants in Neurospora. Mutation Research 80, 43–64.CrossRefGoogle ScholarPubMed
Kafer, E. & Fraser, M. J. (1979). Isolation and genetic analysis of nuclease halos mutants of Neurospora crassa. Molecular and General Genetics 169, 117–127.CrossRefGoogle ScholarPubMed
Kinsey, J. A. & Stadler, D. R. (1969). Interaction between analogue resistance and amino acid auxotrophy in Neurospora. Journal of Bacteriology 97, 1114–1117.CrossRefGoogle ScholarPubMed
Kornberg, A. (1980). DNA Replication. San Francisco: W. H. Freeman.Google Scholar
Lawrence, C. W. & Christensen, R. (1976). UV mutagenesis in radiation-sensitive strains of yeast. Genetics 82, 207–232.CrossRefGoogle ScholarPubMed
Mishra, N. C. (1982). Gene transfer in fungi. Advances in Genetics 22 (In the Press.)Google Scholar
Newmeyer, D. A., Schroeder, A. L. & Galeazzi, D. R. (1978). An apparent connection between histidine, recombination and repair in Neurospora. Genetics 89, 271–279.CrossRefGoogle ScholarPubMed
Radding, C. (1978). Genetic recombination: strand transfer and mismatch repair. Annual Review of Biochemistry 47, 847–880.CrossRefGoogle ScholarPubMed
Schablik, M., Delange, A. M., Shums, A. A. & Mishra, N. C. (1983). Construction of agenomic library of Neurospora crassa. FEMS Microbiology Letters 16, 321–325.CrossRefGoogle Scholar
Schroeder, A. L. (1975). Genetic control of radiation sensitivity and DNA repair in Neurospora. In Molecular Mechanisms of Repair of DNA, part B (ed. Hanawalt, P. C. and Setlow, R. B.), pp. 567–576. New York: Plenum.CrossRefGoogle Scholar
Stadler, D. R. & Mover, R. (1981). Induced repair of genetic damage in Neurospora. Genetics 98, 763–774.CrossRefGoogle ScholarPubMed
Stahl, F. (1980). Genetic Recombination-Thinking About in Phage and Fungi. San Francisco: W. H. Freeman.Google Scholar
Witkin, E. (1976). Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriological Reviews 40, 869–907.CrossRefGoogle ScholarPubMed
Worthy, T. E. & Epler, J. L. (1973). Biochemical basis of radiation-sensitivity in mutants of Neurospora crassa. Mutation Research 69, 167–173.CrossRefGoogle Scholar