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Effects of acriflavine on the transfer of episomes and bacterial chromosome in Escherichia coli K-12

Published online by Cambridge University Press:  14 April 2009

Toshihiko Arai  and
Tsutomu Watanabe
Toshihiko Arai
Affiliation:
Department of Microbiology, Keio University School of Medicine, Tokyo, Japan
Tsutomu Watanabe
Affiliation:
Department of Microbiology, Keio University School of Medicine, Tokyo, Japan
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The effects of acriflavine on the transfer of various episomes and of the bacterial chromosome were studied using acriflavine-sensitive and -resistant strains of E. coli K-12 as donors and recipients. It was found that the inhibition of transfer of these elements by acriflavine was only slight when donor bacteria were resistant to this acridine dye, regardless of whether the recipient bacteria were acriflavine-sensitive or -resistant. This result seems to support the view that acriflavine inhibits the replication of these elements in donor bacteria which is considered essential for their transfer. With colicinogenic factors, in contrast to F and R, the results were affected by the sensitivity or resistance of the recipient and were in support of the killing by acriflavine of the cells which received the colicinogenic factors.

Type
Research Article
Information
Genetics Research , Volume 10 , Issue 3 , December 1967 , pp. 241 - 249
Copyright
Copyright © Cambridge University Press 1967

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience Publishers, Inc.CrossRefGoogle Scholar
Akiba, T., Kato, T. & Ichinohe, F. (1964). Mode of actions of inhibitory substances for resistance transfer. Jap. J. Bact. 19, 313–314 (in Japanese).Google Scholar
Brinton, C. C. Jr, Novotny, C. & Pittman, J. (1966). Nucleic acid conduction by F. pili. Proc. IX Int. Cong. Microbiol., Moscow, p. 70.Google Scholar
Cuzin, F. & Jacob, F. (1966). Inhibition par les acridines du transfert genetique par les souches donatrices d'Escherichia coli K12. Annls Inst. Pasteur, Paris, T111, 427–436.Google Scholar
Frédéricq, P. (1948). Actions antiobiotiques reciproques chez les Enterobacteriaceae. Revue belge Path. M éd. exp. 19, suppl. 4.Google Scholar
Freifelder, D. (1966). Acridine orange- and methylene blue-sensitized induction of Escherichia coli lysogenic for phage λ. Virology. 30, 567–568.CrossRefGoogle ScholarPubMed
Hayashi, K., Kodaira, T., Ogawa, Y., Baba, K. & Iwasaki, K. (1965). Studies on the substances affecting the episomic infective transfer system. (I) The relationship between the chemical structures of acridine derivatives and the inhibitory action on the multiple-drug-resistance transfer. Jap. J. Bact. 20, 498–505 (in Japanese).CrossRefGoogle Scholar
Hirota, Y. (1960). The effect of acridine dyes on mating type factors in Escherichia coli. Proc. natn. Acad. Sci. U.S.A. 46, 57–64.CrossRefGoogle ScholarPubMed
Hirota, Y. & Sneath, P. H. A. (1961). F′ and F mediated transduction in Escherichia coli K-12. Jap. J. Genet. 36, 307–318.CrossRefGoogle Scholar
Hurwitz, J., Furth, J. J., Malamy, M. & Alexander, M. (1962). The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin. Proc. natn. Acad. Sci. U.S.A. 48, 1222–1230.CrossRefGoogle ScholarPubMed
Jacob, F., Brenner, S. & Cuzin, F. (1963). On the regulation of DNA replication in bacteria. Cold Spring Harb. Symp. quant. Biol. 28, 329–348.CrossRefGoogle Scholar
Lorkiewicz, Z., Maciazek, K. & Nackiewicz, Z. (1964). The influence of acriflavine on transfer of the colicinogenic factor. Acta microbiol. pol. 13, 273–281.Google ScholarPubMed
Lorkiewicz, Z., Derylo, M. & Frelik, M. (1965). Studies on colicinogenic factors transfer and elimination. Proc. Symp. on the Mutational Process. The Physiology of Gene and Mutation Expression, Prague, pp. 235–246.Google Scholar
Orgel, A. & Brenner, S. (1961). Mutagenesis of bacteriophage T4 by acridines. J. molec. Biol. 3, 762–768.CrossRefGoogle ScholarPubMed
Ozeki, H., Stocker, B. A. D. & Smith, S. M. (1962). Transmission of colicinogeny between strains of Salmonella typhimurium grown together. J. gen. Microbiol. 28, 671–687.CrossRefGoogle Scholar
Takano, T., Sato, S., Levy, S. B. & Watanabe, T. (1966). Episomic resistance factors in Enterobacteriaceae. 34. The specific effects of the inhibitors of DNA synthesis on the transfer of R factor and F factor. Med. Biol. (Tokyo), 73, 79–83 (in Japanese).Google Scholar
Watanabe, T. (1963). Infective heredity of multiple drug resistance in bacteria. Bact. Rev. 27, 87–115.CrossRefGoogle ScholarPubMed
Watanabe, T. & Fukasawa, T. (1961). Episome-mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes. J. bact. 81, 679–683.CrossRefGoogle ScholarPubMed
Watanabe, T., Nishida, H., Ogata, C., Arai, T. & Sato, S. (1964 a). Episome-mediated transfer of drug resistance in Enterobacteriaceae. VII. Two types of naturally occurring R factors. J. bact. 88, 716–726.CrossRefGoogle ScholarPubMed
Watanabe, T., Ogata, C. & Sato, S. (1964 b). Episome-mediated transfer of drug resistance in Enterobacteriaceae. VIII. Six-drug-resistance R factor. J. bact. 88, 922–928.CrossRefGoogle ScholarPubMed