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Effects of sodium butyrate on X-Ray and bleomycin-induced chromosome aberrations in human peripheral blood lymphocytes

Published online by Cambridge University Press:  14 April 2009

K. Sankaranarayanan ,
A. v. Duyn ,
M. J. Loos ,
R. Meschini  and
A. T. Natarajan
K. Sankaranarayanan*
Affiliation:
MGC Department of Radiation Genetics & Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
A. v. Duyn
Affiliation:
MGC Department of Radiation Genetics & Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
M. J. Loos
Affiliation:
MGC Department of Radiation Genetics & Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
R. Meschini
Affiliation:
MGC Department of Radiation Genetics & Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
A. T. Natarajan
Affiliation:
MGC Department of Radiation Genetics & Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
*
† Corresponding author.

Summary

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Peripheral blood lymphocytes from normal human volunteers or from Down syndrome patients were pre-treated with sodium butyrate (a compound which is known to induce structural modifications in the chromatin through hyperacetylation of nucleosomal core histones) and exposed to X-irradiation or treated with bleomycin in vitro in the G0 and/or G1 stage(s) of the cell cycle. The frequencies of chromosomal aberrations in the first mitosis after treatment were scored.

The results show an enhancement in the yield of aberrations in the butyrate pre-treated groups. However, the absolute frequencies of chromosomal aberrations as well as the relative increases with butyrate pre-treatment varied between blood samples from different donors suggesting the existence of inter-individual variations. There is a parallelism between the effects of X-irradiation or of combined treatments in G0 and G1 stages and between effects observed in the X-ray and bleomycin series. The increase in the yields of chromosomal aberrations in butyrate-treated and X-irradiated lymphocytes (relative to those which received X-irradiation alone) is interpreted as a consequence of the inhibition of repair of DNA damage by butyrate.

Type
Research Article
Information
Genetics Research , Volume 56 , Issue 2-3 , October 1990 , pp. 267 - 276
Copyright
Copyright © Cambridge University Press 1990

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