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Genetic studies of strain Bs8 of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

John Donch  and
Joseph Greenberg
John Donch
Affiliation:
Pala Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, California 94301
Joseph Greenberg
Affiliation:
Pala Alto Medical Research Foundation, 860 Bryant Street, Palo Alto, California 94301
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Strain Bs8 is a u.v.-sensitive derivative of strain B. It is unable to reactivate irradiated phage (HCR) and it cannot be induced to form filaments. The HCR properties are attributable to a gene, uvr8, cotransducible with gal+. When uvr8 was transduced into radiation-resistant strain B/r the resulting phenotype was indistinguishable from Bs8. When transduced into a lon K-12 strain the phenotype was more sensitive than Bs8, filament-inducible and mucoid. When PI-Bs8 was used to transduce proC+ into a proC K-12 strain, 4% of the transductants were lon, i.e. about as u.v.-sensitive as Ion K-12, filament-inducible and mucoid. Radiation resistance could not be transduced from strain Bs8 with proC+ into a proC lon K-12 derivative. Nor did Bs8 show any evidence of being exr. Bs8 is a double mutant of strain B, behaving as though it were HCE, in a B/r background.

Type
Research Article
Information
Genetics Research , Volume 12 , Issue 1 , August 1968 , pp. 55 - 63
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience Publishers, Inc.CrossRefGoogle Scholar
Chung, Y. S. & Greenberg, J. (1968). Genes affecting sensitivity to ultra-violet light in the malB region of the chromosome of Escherichia coli. Genetics (in the Press).Google Scholar
Cummings, D. J. & Mondale, L. (1967). Thymineless death in Escherichia coli: strain specificity. J. Bact. 93, 1917–1924.CrossRefGoogle ScholarPubMed
Demerec, M., Adelberg, E. A., Clark, A. J. & Hartman, P. E. (1966). A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76.CrossRefGoogle ScholarPubMed
Donch, J., Chung, Y. S. & Greenberg, J. (1968). Locus for radiation resistance in Escherichia coli strain B/r (in the Press)Google Scholar
Donch, J. & Greenberg, J. (1968 a). Loci of radiation sensitivity in Bs strains of Escherichia coli. Genet. Res. (in the Press).Google ScholarPubMed
Donch, J. & Greenberg, J. (1968 b). The UV sensitivity gene of Escherichia coli strain B. J. Bact. (in the Press).CrossRefGoogle Scholar
Greenberg, J. (1964). A locus for radiation resistance in Escherichia coli. Genetics 49, 771–778.Google ScholarPubMed
Greenberg, J. (1967). Loci for radiation sensitivity in Escherichia coli strain Bs – 1. Genetics 55, 193–201.CrossRefGoogle ScholarPubMed
Hill, R. F. & Feiner, R. R. (1964). Further studies of ultraviolet-sensitive mutants of Escherichia coli strain B. J. gen. Microbiol. 35, 105–114.CrossRefGoogle ScholarPubMed
Hill, R. F. & Simson, E. (1961). A study of radiosensitive and radio-resistant mutants of Escherichia coli strain B. J. gen. Microbiol. 24, 1–14.CrossRefGoogle Scholar
Howard-Flanders, P., Boyce, R. P. & Theriot, L. (1966). Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics, 53, 1119–1136.CrossRefGoogle ScholarPubMed
Howard-Flanders, P., Simson, E. & Theriot, L. (1964). A locus that controls filament formation and sensitivity to radiation in Escherichia coli K-12. Genetics 49, 237–246.CrossRefGoogle ScholarPubMed
Markovitz, A. (1964). Regulatory mechanisms for synthesis of capsular polysaccharide in mucoid mutants of Escherichia coli K 12. Proc. Natn. Acad. Sci. U.S. 51, 239–246.CrossRefGoogle Scholar
Markovitz, A. & Baker, B. (1967). Suppression of radiation sensitivity and capsular polysaccharide synthesis in Escherichia coli K 12 by ochre suppressors. J. Bact. 94, 388–395.Google ScholarPubMed
Witkin, E. M. (1947). Genetics of resistance to radiation in Escherichia coli. Genetics 32, 221–248.Google ScholarPubMed