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Glutamyl-γ-methyl ester acts as a methionine analogue in Escherichia coli: analogue resistant mutants map at the metJ and metK loci

Published online by Cambridge University Press:  14 April 2009

Jan Kraus ,
Dieter Soll  and
K. Brooks Low
Jan Kraus
Affiliation:
Department of Molecular Biophysics and Biochemistry
Dieter Soll
Affiliation:
Department of Molecular Biophysics and Biochemistry
K. Brooks Low
Affiliation:
Department of Therapeutic Radiology, Yale University, New Haven, Connecticut USA 06510
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Escherichia coli K-12 mutants resistant to glutamyl-γ-methyl ester were isolated. A mutation leading to resistance of up to 1·4 mg/ml of the methionine analogue maps at min 63 and is 13% cotransducible with serA indicating an alteration in the metK gene. Another mutation leading to resistance to 3 mg/ml of the analogue and cross-resistance to other amino acid analogues maps at min 87. This mutation, which has the phenotype of MetJ−, is shown to be situated between the glpK and metB genes and thus indicates a different gene order from the published one.

Type
Research Article
Information
Genetics Research , Volume 33 , Issue 1 , February 1979 , pp. 49 - 55
Copyright
Copyright © Cambridge University Press 1979

References

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