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The in vivo and in vitro transmission frequencies of the tw5-haplotype in mice

Published online by Cambridge University Press:  14 April 2009

William Garside  and
Nina Hillman
William Garside
Affiliation:
Department of Biology, Temple University, Philadelphia, PA 19122
Nina Hillman
Affiliation:
Department of Biology, Temple University, Philadelphia, PA 19122
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Summary

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The recessive tω5-haplotype, a complete haplotype, is transmitted by heterozygous male mice at very high frequencies (> 0·90) in normal matings. The present studies were undertaken to determine the effects of delayed matings and in vitro fertilizations on this phenotypic expression. Males carrying the tω5-haplotype ( + / tω5) were first tested for their frequencies of transmission of the mutant 17th chromosome in both normal and delayed matings. Spermatozoa obtained from these same males were then used to fertilize eggs in vitro. The in vivo and in vitro transmission frequencies were found to be statistically equivalent in all types of inseminations. An in vitro fertilization time course study showed that the same percentages of eggs are fertilized by tω5- bearing spermatozoa when the gametes are coincubated for either 2 or 6 h. The data lead to the conclusion that the transmission frequency of the tω5-haplotype is not affected either by the length of time elapsing between insemination and fertilization or by the environment in which fertilization occurs.

Type
Research Article
Information
Genetics Research , Volume 53 , Issue 1 , February 1989 , pp. 21 - 24
Copyright
Copyright © Cambridge University Press 1989

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