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Mutagenesis in Escherichia coli

III. Requirement for DNA synthesis in mutation by gamma rays of T4-phage complexed with Escherichia coli

Published online by Cambridge University Press:  14 April 2009

B. A. Bridges ,
Rachel E. Dennis  and
R. J. Munson
B. A. Bridges
Affiliation:
M.R.C. Radiology Unit, Harwell, Didcot, Berkshire, U.K.
Rachel E. Dennis
Affiliation:
M.R.C. Radiology Unit, Harwell, Didcot, Berkshire, U.K.
R. J. Munson
Affiliation:
M.R.C. Radiology Unit, Harwell, Didcot, Berkshire, U.K.
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A system has been developed for the study of reversion of an amber mutation responsible for a deficiency in DNA synthesis in T4 phage E51. When complexed with bacteria able to suppress the amber mutation the induced mutation rate per phage genome per rad is

When complexed with bacteria unable to suppress the amber mutation (and being thus unable to synthesize phage DNA) the induced mutation rate is at least 14 times lower indicating that DNA synthesis is necessary for the production of the majority of functional reversions at the amber site. The induced mutation rate in suppressor-containing bacteria is independent of multiplicity of infection between 0·2 and 5, suggesting that recombination immediately after irradiation between phage genomes is unlikely to be a requirement for the mutation process.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 2 , April 1970 , pp. 147 - 156
Copyright
Copyright © Cambridge University Press 1970

References

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