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The time course of recombinant production in Streptomyces coelicolor

Published online by Cambridge University Press:  14 April 2009

G. Sermonti ,
A. Maria Puglia  and
Giuseppina Ficarra
G. Sermonti
Affiliation:
Istituto di Genetica, Università di Palermo, Italy
A. Maria Puglia
Affiliation:
Istituto di Genetica, Università di Palermo, Italy
Giuseppina Ficarra
Affiliation:
Istituto di Genetica, Università di Palermo, Italy
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Summary

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The process leading to gene recombination can be interrupted in the filamentous bacteria Streptomyces coelicolor by growing mixed cultures on cellophane disks lying on complete medium. The mycelium is harvested, broken, diluted and the broken hyphae plated at different time intervals. By this means some markers can be excluded from heteroclones or from recombinant progeny in early samples. The recombinant pattern clearly changes with time, with an increase of markers contributed to the recombinant progeny. In crosses between male (NF) and female (UF) strains, the maleness is the first donor trait to appear in the cells of the recipient parent. The fertility factor does not produce a transfer origin on the donor chromosomes; the donor contribution may extend on either side or on both sides of the factor which appears to be compulsory for zygote formation. The longer the time of contact between parental cells, the longer the segment of the donor chromosome contributing to the recombinant progeny. When spores are formed they contain almost exclusively recombinant nuclei derived from segregation processes.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 2 , October 1971 , pp. 133 - 145
Copyright
Copyright © Cambridge University Press 1971

References

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