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The use of retrotransposons as markers for mapping genes responsible for fitness differences between related Drosophila melanogaster strains

Published online by Cambridge University Press:  14 April 2009

Sergey V. Nuzhdin ,
Peter D. Keightley  and
Elena G. Pasyukova
Sergey V. Nuzhdin
Affiliation:
Institute of Molecular Genetics, Kurchatov Sq. 46, 123182, Moscow, Russia Department of Genetics, Box 7614, North Carolina State University, Raleigh, NC 27695, USA
Peter D. Keightley
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland
Elena G. Pasyukova
Affiliation:
Institute of MolecularGenetics, Kurchatov Sq. 46, 123182, Moscow, Russia
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Hitch-hiking of dispersed mobile elements serving as molecular markers was used as a new tool for mapping quantitative trait loci in Drosophila melanogaster. Two Drosophila strains with high fitness (HA) were backcrossed repeatedly to a closely related strain with low fitness (LA) to initiate experimental populations with expected HA gene frequencies of 1/32. The frequencies of 19 insertion sites of the retrotransposons mdg1 and copia were analyzed after 11 to 17 generations. Frequencies of sites from the HA line increased substantially in the pericentromeric region, indicating that one or more loci responsible for the fitness difference between the strains were located there. A maximum likelihood (ML) procedure was applied to estimate selection coefficients associated with the markers, and this indicated a broad, strongly selected region of the chromosome. At least one additional locus was localized in the middle of the 2L arm. Possible applications of this method are discussed.

Type
Research Article
Information
Genetics Research , Volume 62 , Issue 2 , October 1993 , pp. 125 - 131
Copyright
Copyright © Cambridge University Press 1993

References

Ananiev, E. V., Belyaeva, E. S., Gvozdev, V. A., Tchurikov, N. A., Ilyin, Y. V. & Georgiev, G. P. (1979). Scattered reiterated genes of Drosophila melanogaster with varying location. II Location of Dm 225 and Dm 234 genes at the regions of intercalary heterochromatin. Genetika (Moscow) 15, 785–799.Google Scholar
Beckman, J. S. & Soller, M. (1983). Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theoretical and Applied Genetics 67, 35–41.CrossRefGoogle Scholar
Belyaeva, E. S., Ananiev, E. V. & Gvozdev, V. A. (1984). Distribution of mobile dispersed genes (mdg1 and mdg3) in the chromosomes of Drosophila melanogaster. Chromosoma 90, 16–19.CrossRefGoogle Scholar
Belyaeva, E. S., Pasyukova, E. G., Glushkova, I. V., Iovleva, O. V., Kaidanov, L. Z. & Gvozdev, V. A. (1989). Hot spots of mdgl locations in chromosome 2 and fitness level in Drosophila melanogaster stocks of common origin. Genetika (Moscow) 25, 1047–1059.Google Scholar
Charlesworth, B., Lapid, A. & Canada, D. (1992). The distribution of transposable elements within and between chromosomes in a population of Drosophila melanogaster. II. Inferences on the nature of selection against elements. Genetical Research 60, 115–130.CrossRefGoogle Scholar
Dunsmuir, P., Brorein, W. Y., Simon, M. A. & Rubin, G. M. (1980). Insertion of Drosophila transposable element copia generates a 5 base pair duplication. Cell 21, 575–579.CrossRefGoogle ScholarPubMed
Georgiev, G. P., Ylyin, Y. V., Chmeliauskaite, V. G., Ryskov, A. P., Kramerov, D. A., Skryabin, K. G., Krayev, A. S., Lukanidin, E. M. & Grigorian, M. S. (1981). Mobile dispersed genetic elements and other middle repetitive DNA sequences in the genomes of Drosophila and mouse: transcription and biological significance. Cold Spring Harbor Symposia on Quantitative Biology 45, 641–653.CrossRefGoogle ScholarPubMed
Hart, D. L. & Haymer, D. S. (1983). Measure of fitness in Drosophila. Stadler Symposia 15, 43–58.Google Scholar
Kaidanov, L. Z. (1980). The analysis of genetic consequences of selection and inbreeding in Drosophila melanogaster. Genetica 52/53, 165–181.Google Scholar
Keightley, P. D. & Bulfield, G. (1993). Location of quantitative trait loci in mouse lines selected for increased and decreased body size. Submitted to Genetical Research.Google Scholar
Lander, E. S. & Botstein, D. (1989). Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 122, 185–199.CrossRefGoogle Scholar
Lebowitz, R. J., Soller, M. & Beckmann, J. S. (1987). Trait-based analysis for the detection of linkage between marker loci and quantitative trait loci in crosses between inbred lines. Theoretical and Applied Genetics 73, 556–561.CrossRefGoogle ScholarPubMed
Mackay, T. F. C., Lyman, R. & Jackson, M. S. (1992). Effects of P element insertions on quantitative traits in Drosophila melanogaster. Genetics 130, 315–332.CrossRefGoogle ScholarPubMed
Mather, K. & Jinks, J. (1982). London: Biometrical Genetics.CrossRefGoogle Scholar
McMillan, J. & Robertson, A. (1974). The power of methods of the detection of major genes affecting quantitative characters. Heredity 32, 349–356.CrossRefGoogle ScholarPubMed
Nuzhdin, S. V. & Pasyukova, E. G. (1991). New approach to polygene mapping. Location of genes controlling fitness level in related Drosophila melanogaster stocks. Genetika (Moscow) 27, 849–859.Google Scholar
Pasyukova, E. G., Belyaeva, E. S., Kogan, G. L., Kaidanov, L. Z. & Gvozdev, V. A. (1986). Concerted transpositions of mobile genetic elements coupled with fitness changes in Drosophila melanogaster. Molecular Biology and Evolution 3, 299–312.Google ScholarPubMed
Pasyukova, E. G., Belyaeva, E. S., Ilyinskaya, L. E. & Gvozdev, V. A. (1989). Outcross-dependent transpositions of copia-like mobile genetic elements in chromosomes of an inbred Drosophila melanogaster stock. Molecular and General Genetics 212, 281–286.CrossRefGoogle Scholar
Paterson, A. H., Lander, E. S., Hewitt, J. D., Paterson, S., Linkoln, S. E. & Tanksley, S. D. (1988). Resolution of quantitative traits into Mendelian factors by using a complete RFLP linkage map. Nature 335, 721–726.CrossRefGoogle Scholar
Sax, K. (1923). The association of size differences with seed-coat pattern and pigmentation in Phaseolus vulgaris. Genetics 28, 552–560.CrossRefGoogle Scholar
Shrimpton, A. E. & Robertson, A. (1988 a). The isolation of polygenic factors controlling bristle score in Drosophila melanogaster. I. Allocation of third chromosome sterno-pleural bristle effects within chromosome sections. Genetics 118, 437–443.CrossRefGoogle Scholar
Shrimpton, A. E. & Robertson, A. (1988 b). The isolation of polygenic factors controlling bristle score in Drosophila melanogaster. II. Distribution of third chromosome bristle effects within chromosome sections. Genetics 118, 445–459.CrossRefGoogle ScholarPubMed
Stuber, C. W., Moll, R. H., Goodman, M. M., Schaffer, H. E. & Weir, B. S. (1980). Allozyme frequency changes associated with selection for increased grain yield in maize. Genetics 95, 225–236.CrossRefGoogle ScholarPubMed
Tanksley, S. D., Medina-Filho, H. & Rick, C. M. (1982). Use of naturally occurring enzyme variation to detect and map genes controlling quantitative traits in an interspecific backross of tomato. Heredity 49, 11–27.CrossRefGoogle Scholar
Tchurikov, N. A., Ilyin, Y. V., Skryabin, K. G., Ananiev, E. V., Bayev, A. A., Krayev, A. S., Zelentsova, E. S., Kulguskin, V. V., Lyubamirskaya, N. V. & Georgiev, G. P. (1981). Mobile dispersed genetic elements in the genome of Drosophila melanogaster. Cold Spring Harbor Symposia on Quantitative Biology 45, 655–665.CrossRefGoogle ScholarPubMed
Thoday, J. M. (1979). Polygene mapping: uses and limitations. In Quantitative Genetic Variation (ed. Thompson, J. N. and Thoday, J. M.), pp. 219–233. New York: Academic Press, 191, 368–370.CrossRefGoogle Scholar
Thomson, C. (1977). The effect of a selected locus on linked neutral loci. Genetics 285, 753–788.CrossRefGoogle Scholar