Hostname: page-component-76fb5796d-vfjqv Total loading time: 0 Render date: 2024-04-26T12:32:11.423Z Has data issue: false hasContentIssue false
  • English
  • Français

Room Decontamination with UV Radiation

Published online by Cambridge University Press:  02 January 2015

William A. Rutala ,
Maria F. Gergen  and
David J. Weber
William A. Rutala*
Affiliation:
Hospital Epidemiology, University of North Carolina Health Care, Chapel Hill, North Carolina Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina
Maria F. Gergen
Affiliation:
Hospital Epidemiology, University of North Carolina Health Care, Chapel Hill, North Carolina
David J. Weber
Affiliation:
Hospital Epidemiology, University of North Carolina Health Care, Chapel Hill, North Carolina Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina
*
Division of Infectious Diseases, Bioinformatics, University of North Carolina at Chapel Hill, 130 Mason Farm Road, Chapel Hill, NC 27599-7030, (brutala@unch.unc.edu)
Get access Rights & Permissions [Opens in a new window]

Extract

Objective.

To determine the effectiveness of a UV-C-emitting device to eliminate clinically important nosocomial pathogens in a contaminated hospital room.

Methods.

This study was carried out in a standard but empty hospital room (phase 1) and in a room previously occupied by a patient with methicillin-resistant Staphylococcus aureus (MRSA) or vancomycin-resistant Enterococcus (VRE) infection (phase 2) in an acute care tertiary hospital in North Carolina from January 21 through September 21, 2009. During phase 1, 8 × 8 cm Formica sheets contaminated with approximately 104-105 organisms of MRSA, VRE, multidrug-resistant (MDR) Acinetobacter baumannii, or Clostridium difficile spores were placed in a hospital room, both in direct line of sight of the UV-C device and behind objects. After timed exposure, the presence of the microbes was assessed. During phase 2, specific sites in rooms that had housed patients with MRSA or VRE infection were sampled before and after UV-C irradiation. After timed exposure, the presence of MRSA and VRE and total colony counts were assessed.

Results.

In our test room, the effectiveness of UV-C radiation in reducing the counts of vegetative bacteria on surfaces was more than 99.9% within 15 minutes, and the reduction in C. difficile spores was 99.8% within 50 minutes. In rooms occupied by patients with MRSA, UV-C irradiation of approximately 15 minutes duration resulted in a decrease in total CFUs per plate (mean, 384 CFUs vs 19 CFUs; P < .001), in the number of samples positive for MRSA (81 [20.3%] of 400 plates vs 2 [0.5%] of 400 plates; P < .001 ), and in MRSA counts per MRSA-positive plate (mean, 37 CFUs vs 2 CFUs; P < .001).

Conclusions.

This UV-C device was effective in eliminating vegetative bacteria on contaminated surfaces both in the line of sight and behind objects within approximately 15 minutes and in eliminating C. difficile spores within 50 minutes.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 31 , Issue 10 , October 2010 , pp. 1025 - 1029
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Carling, PC, Briggs, JL, Perkins, J, Highlander, D. Improved cleaning of patient rooms using a new targeting method. Clin Infect Dis 2006;42:385–388.CrossRefGoogle ScholarPubMed
2.Carling, PC, Parry, MF, Rupp, ME, et al.Improving cleaning of the environment surrounding patients in 36 acute care hospitals. Infect Control Hosp Epidemiol 2008;29:1035–1041.CrossRefGoogle ScholarPubMed
3.Carling, PC, Parry, MF, Von Beheren, SM; Healthcare Environmental Hygiene Study Group. Identifying opportunities to enhance environmental cleaning in 23 acute care hospitals. Infect Control Hosp Epidemiol 2008;29:1–7.Google Scholar
4.Huang, SS, Datta, R, Platt, R. Risk of acquiring antibiotic-resistant bacteria from prior room occupants. Arch Intern Med 2006;166:1945–1951.Google Scholar
5.Drees, M, Snydman, DR, Schmid, CH, et al.Prior environmental contamination increases the risk of acquisition of vancomycin-resistant en-terococci. Clin Infect Dis 2008;46:678–685.CrossRefGoogle Scholar
6.Shaughnessy, M, Micielli, R, Depestel, D, et al.Evaluation of hospital room assignment and acquisition of Clostridium difficile associated diarrhea (CDAD). In: Programs and abstracts of the 48th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy/Infections Disease Society of America 46th Annual Meeting. Washington, DC: American Society for Microbiology, 2008. Abstract K-4194.Google Scholar
7.Boyce, JM. New approaches to decontamination of rooms after patients are discharged, infect Control Hosp Epidemiol 2009;30:515–517.Google Scholar
8.Owens, MU, Deal, DR, Shoemaker, MO, Knudson, GB, Meszaros, IE, Deal, JL. High-dose ultraviolet C light inactivates spores of Bacillus subtilis varniger and Badllus anthracis Sterne on non-reflective surfaces. Appl Biosaf 2005;10:240–247.CrossRefGoogle Scholar
9.Belbian, A. Disinfectants. In: Cunniff, P, ed. Official Methods of Analysis of the AOAC International. Vol 1. Gaithersburg, Maryland: AOAC International, 1999: § 6–1-6-18.Google Scholar
10.Rastogi, VK, Wallace, L, Smith, LS. Disinfection of Acinetobacter baumannii-contammated surfaces relevant to medical treatment facilities with ultraviolet C light. Mil Med 2007;172:1166–1169.Google Scholar
11.Blatchley, ER III, Peel, MM. Disinfection by ultraviolet irradiation. In: Block, SS, ed. Disinfection, Sterilization and Preservation. Philadelphia, Pennsylvania: Lippincott Williams & Wilkins, 2001:823–851.Google Scholar
12.Shechmeister, IL. Sterilization by ultraviolet irradiation. In: Block, SS, ed. Disinfection, Sterilization and Preservation. Philadelphia, Pennsylvania: Lea &c Febiger, 1991:553–565.Google Scholar
13.Rutala, WA, Weber, DJ. Cleaning, disinfection and sterilization. In: Carrico, R, ed. APIC Text of Infection Control and Epidemiology. Washington, DC: Association for Professionals in Infection Control and Epidemiology, 2009:21:1–21:27.Google Scholar