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Antimicrobial Activity of Glucoprotamin: A Clinical Study of a New Disinfectant for Instruments

Published online by Cambridge University Press:  02 January 2015

Andreas F. Widmer  and
Reno Frei
Andreas F. Widmer*
Affiliation:
Division of Infectious Diseases and Hospital Epidemiology, University of Basel Hospitals & Clinics, Basel, Switzerland
Reno Frei
Affiliation:
Bacteriology Laboratory, University of Basel Hospitals & Clinics, Basel, Switzerland
*
University Hospital Basel, Division of Hospital Epidemiology, Petersgraben 4, 4031 Basel, Switzerland
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Abstract

Objective:

To determine the in vitro efficacy of glucoprotamin for the disinfection of instruments.

Design:

Prospective observational study.

Setting:

University women's hospital.

Methods:

Instruments were immersed in saline solution after use, and glucoprotamin was added to a concentration of 1.5% before soaking for 60 minutes. Biocidal activity was determined by the difference in colony-forming units (CFU) on instruments before and after disinfection.

Results:

One hundred thirty-seven instruments were collected during 10 days and exposed to a 1.5% dilution of glucoprotamin without prior washing. Bioburden before disinfection ranged from 2 × 105 to 7.1 × 107 CFU per instrument. Average bacterial killing was 5.98 log10 CFU ± 0.48 under aerobic conditions and 6.75 log10 CFU ± 0.54 under anaerobic conditions, despite the presence of large amounts of proteins on instruments that were frequently bloody. No vegetative bacteria were isolated in any sample after disinfection.

Conclusion:

This clinical study confirmed excellent in vitro efficacy of glucoprotamin without prior removal of proteins and debris.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 24 , Issue 10 , October 2003 , pp. 762 - 764
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2003

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