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Molecular Typing of Mycobacterium chelonae Isolates From a Pseudo-outbreak Involving an Automated Bronchoscope Washer

Published online by Cambridge University Press:  02 January 2015

Alexandra Chroneou
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington
Sarah K. Zimmerman
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington Department of Microbiology and Laboratory Medicine, Lahey Clinic Medical Center, Burlington
Steven Cook
Affiliation:
Department of Microbiology and Laboratory Medicine, Lahey Clinic Medical Center, Burlington
Sandra Willey
Affiliation:
Infection Control and Hospital Epidemiology, Lahey Clinic Medical Center, Burlington
Jane Eyre-Kelly
Affiliation:
Infection Control and Hospital Epidemiology, Lahey Clinic Medical Center, Burlington
Nikolaos Zias
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington
Daniel S. Shapiro
Affiliation:
Infectious Diseases, Lahey Clinic Medical Center, Burlington Department of Microbiology and Laboratory Medicine, Lahey Clinic Medical Center, Burlington Boston University School of Medicine, Boston, Massachusetts
John F. Beamis
Affiliation:
Departments of Pulmonary and Critical Care Medicine, Lahey Clinic Medical Center, Burlington Tufts University School of Medicine, Boston, Massachusetts
Donald E. Craven*
Affiliation:
Infectious Diseases, Lahey Clinic Medical Center, Burlington Tufts University School of Medicine, Boston, Massachusetts
*
Department of Infectious Diseases, Lahey Clinic Medical Center, 41 Mall Rd., Burlington, MA 01805 (donald.e.craven@lahey.org)

Abstract

We describe a pseudo-outbreak of Mycobacterium chelonae infection in bronchoalveolar lavage fluid from 9 patients that was traced to contamination of an automated bronchoscope washer. Molecular typing using repetitive extragenic palindromic polymerase chain reaction was helpful in confirming epidemiologic and clinical findings.

Type
Concise Communication
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2008

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