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Viability of Staphylococci in Various Diluents

Published online by Cambridge University Press:  02 January 2015

Razmick Essiain
Affiliation:
Department of Clinical Laboratory Sciences, College of Allied Health, University of Oklahoma Health Sciences Center, and the Clinical Microbiology Section, Veterans Administration Medical Center, Oklahoma City, OK 73104
D.J Flournoy*
Affiliation:
Department of Clinical Laboratory Sciences, College of Allied Health, University of Oklahoma Health Sciences Center, and the Clinical Microbiology Section, Veterans Administration Medical Center, Oklahoma City, OK 73104
*
VA Medical Center (113), 921 NE 13th Street, Oklahoma City, OK 73104

Abstract

Standardized suspensions of 78 staphylococci and micrococci were stored in different diluents and tested periodically for 3 weeks to determine their ability to maintain viability. In 92% of the tests, diluent suspensions yielded viable organisms for at least 21 days. Organism survival was maintained in the following order, from best to worst: skim milk, tap water, 0.9% NaCl, deionized water, Dianeal (peritoneal dialysis) fluid, 0.2% bovine serum albumin and 5% glycerol. In 81% of the instances, oxacillin-susceptible staphylococci survived better than oxacillin-resistant staphylococci.

Type
Original Articles
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1986

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