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Growth-regulatory activity of silver fern extract on the cotton bollworm, Helicoverpa armigera (Hübner)

Published online by Cambridge University Press:  19 September 2011

A. Josephrajkumar ,
B. Subrahmanyam  and
C. Devakumar
A. Josephrajkumar
Affiliation:
Division of Entomology, Indian Agricultural Research Institute New Delhi-110 012, India
B. Subrahmanyam
Affiliation:
Division of Entomology, Indian Agricultural Research Institute New Delhi-110 012, India
C. Devakumar*
Affiliation:
Agricultural Chemicals, Indian Agricultural Research Institute New Delhi-110 012, India
*
*Corresponding author: BS. E-mail: bhattiprolu_s@yahoomail.com.
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Abstract

Methanolic extracts of stems and roots of silver fern, Cheilanthes farinosa Kaulf., (Polypodiaceae: Pteridophyta) incorporated into a semi-synthetic diet significantly extended the larval period, reduced pupal weight and adversely affected pupation of the cotton bollworm, Helicoverpa armigera (Hübner) (Noctuidae: Lepidoptera). Early third instars were more susceptible to the treatment than late third instars. A steroidal fraction (Rf 0.7) from the methanolic extract significantly reduced mean larval weight but did not affect pupation. A major ecdysteroidal component (R.t. 9.233 min.) was purified by reverse phase HPLC of this fraction, along with two minor components. The total yield of ecdysteroids was 564 mg/kg of shade-dried root and stem matter. Pooled HPLC fractions, when injected into final instars, resulted in dose-dependent effects on larval-pupal transformation. Adults emerging from treated larvae failed to lay eggs and were shorter-lived than their untreated counterparts.

Keywords

phytoecdysteroidsilver fernCheilanthes farinosaHelicoverpa armigeragrowth inhibitorphytoecdystéroïdesfougère cendréeCheilanthes farinosaHelicoverpa armigerainhibiteur de croissance
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 20 , Issue 4 , December 2000 , pp. 295 - 302
Copyright
Copyright © ICIPE 2000

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