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Insulin-induced effect on diapausing pupae of tropical tasar silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae)

Published online by Cambridge University Press:  19 September 2011

Ashok Kumar Sinha ,
Dipankar Chakraborty  and
Anathbandhu Chaudhuri
Ashok Kumar Sinha
Affiliation:
Central Tasar Research and Training Institute, Nagri, Ranchi-835303, India
Dipankar Chakraborty
Affiliation:
Central Tasar Research and Training Institute, Nagri, Ranchi-835303, India
Anathbandhu Chaudhuri
Affiliation:
Central Tasar Research and Training Institute, Nagri, Ranchi-835303, India
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Abstract

Diapausing pupae of tasar silkworm, Antheraea mylitta Drury were injected with ox pancreas insulin (40 I.U. per ml) on day 150 to observe the effect of this vertebrate hormone on diapause physiology. Insulin at the dose of 5 μl (0.2 I.U.) per pupa exhibited its positive impact by enhancing the incidence of male and female moth eclosion, female moth weight, egg production (laid + unlaid), and fecundity and shortening of time taken for emergence in both sexes. But, 10 μl (0.4 I.U.) dose of insulin increased only the female eclosion and reduced the time taken for emergence in each sex when compared to that of control. Administration of higher dose of insulin (10 μ1 per pupa) decreased the egg production and fecundity. No significant changes were observed in hatchability and incubation phenomena of eggs laid by the treated females. Further, both the doses of insulin were able to bring about the morphometric changes of testis and ovary by altering their shape and size during pupal diapause. Thus, it reveals from the preliminary findings that vertebrate insulin induces diapause physiology of A. mylitta.

Résumé

Des pupes en diapause du ver à soie Antheraea mylitta Drury ont reçu au 150e jour une injection d'insuline pancréatique de taureau (40 I.U. par ml) en vue d'enregistrer l'effet de cette hormone sur la physiologie de la diapause. L'insuline à la dose de 5 μl (0,2 I.U.) par pupe a eu un impact positif en augmentant l'incidence de l'éclosion chez les populations mâles et femelles, le poids de la femelle, la production d'oeufs (pondu et non-pondu) et la fécondité ainsi qu'un temps réduit d'émergence chez les deux sexes. Mais une dose de 10 μl (0,4 I.U.) d'insuline a accru seulement l'éclosion de la femelle et réduit la durée jusqu'a l'émergence pour chaque sexe comparativement à celle du témoin. L'administration d'une dose plus élevée d'insuline (10 μl par pupe) a réduit la production d'oeufs et la fecondité. Aucun changement significatif n'a été observé dans l'éclosion et I'incubation d'oeufs pondues par les femelles traitées. En outre, toutes les deux doses d'insuline ont entrainé des changements morphométriques des testicules et des ovaires en modifiant leur forme et leur taille durant la diapause pupale. Par conséquent, ces résultats préliminaires révèlent que l'insuline des vertébrés induit une diapause chez A. mylitta.

Keywords

InsulinAntheraea mylittadiapauseemergencefecundityhatchinggonad
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 14 , Issue 5-6 , December 1993 , pp. 707 - 712
Copyright
Copyright © ICIPE 1993

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