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Larvicidal activity of the α-amylase inhibitor from the seeds of Macrotyloma uniflorum (Leguminosae) against Aedes aegypti (Diptera: Culicidae)

Published online by Cambridge University Press:  10 May 2011

Laxmi Gupta ,
Sudhakar Deshpande ,
Vrushali Tare  and
Sushma Sabharwal
Laxmi Gupta
Affiliation:
Department of Chemistry, Division of Biochemistry, University of Pune, Ganeshkhind Road, Pune411 007, India
Sudhakar Deshpande
Affiliation:
Entomology Section, National Chemical Laboratory, Pune411 008, India
Vrushali Tare
Affiliation:
Entomology Section, National Chemical Laboratory, Pune411 008, India
Sushma Sabharwal*
Affiliation:
Department of Chemistry, Division of Biochemistry, University of Pune, Ganeshkhind Road, Pune411 007, India
*
*E-mail: ssab@chem.unipune.ac.in
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Abstract

The objective of the present study was to evaluate the larvicidal potential of the α-amylase inhibitor from the seeds of Macrotyloma uniflorum (Lam.) Verdc. against Aedes aegypti (L.) larvae. Macrotyloma uniflorum α-amylase inhibitor (MUAI) was partially purified using ion exchange chromatography on a carboxymethyl cellulose column. MUAI at concentrations of 20, 60, 100 and 200 ppm was tested against different instar larvae, pupae and adults of A. aegypti to establish its bioefficacy. MUAI showed a strong larvicidal and a moderate adulticidal effect at a concentration of 200 ppm but did not exhibit pupicidal and ovicidal activities. MUAI-treated larvae showed lower α-amylase activity. MUAI also exhibited ovipositional deterrence activity. Dose-dependent mortality was observed, being highest in the first and second instar larvae. The results thus obtained provide a safe and eco-friendly means in the biological control of A. aegypti larvae.

Keywords

adulticidalAedes aegyptilarvicidalMacrotyloma uniflorum α-amylase inhibitoroviposition deterrence
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 31 , Issue 1-2 , June 2011 , pp. 69 - 74
Copyright
Copyright © ICIPE 2011

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References

Barreau, C., Conrad, J., Fischer, E., Lujan, H. D. and Vernic, K. D. (1999) Identification of surface molecules on salivary glands of the mosquito, Aedes aegypti, by a panel of monoclonal antibodies. Insect Biochemistry and Molecular Biology 29, 515–526.CrossRefGoogle ScholarPubMed
Bernfeld, P. (1955) Amylases α and β. Methods in Enzymology 1, 149–158.CrossRefGoogle Scholar
Brooks, G. T. (2001) Interactions with the gamma-aminobutyric acid A-receptor: polychlorocycloalkanes and recent congeners, pp. 1131–1156. In Handbook of Pesticide Toxicology: Principles, 2nd edn(edited by R. Krieger; Associate Editors: J. Doull, D. Ecobichon, D. Gammon, E. Hodgson, L. Reiter and J. Ross). Academic Press, San Diego/London.Google Scholar
de Omena, M. C., Navarro, D. M. A. F., de Paula, J. E., Luna, J. S., Ferreira de Lima, M. R. and Sant'Ana, A. E. G. (2007) Larvicidal activities against Aedes aegypti of some Brazilian medicinal plants. Bioresource Technology 98, 2549–2556.CrossRefGoogle ScholarPubMed
Finney, D. J. (1971) Probit Analysis. 3rd edn. 333 pp. Cambridge University Press, Cambridge.Google Scholar
Garcez, W. S., Garcez, F. R., da Silva, L. M. G. E. and Hamerski, L. (2009) Larvicidal activity against Aedes aegypti of some plants native to the West-Central region of Brazil. Bioresource Technology 100, 6647–6650.CrossRefGoogle Scholar
Hanson, S. M. and Craig, G. B. Jr (1995) Aedes albopictus (Diptera: Culicidae) eggs: field survivorship during northern Indiana winters. Journal of Medical Entomology 32, 599–604.CrossRefGoogle ScholarPubMed
Hawley, W. A., Pumpuni, C. B., Brady, R. H. and Craig, G. B. Jr (1989) Overwintering survival of Aedes albopictus (Diptera: Culicidae) eggs in Indiana. Journal of Medical Entomology 26, 122–129.CrossRefGoogle ScholarPubMed
Hwang, Y.-S., Schultz, G. W., Axelord, H., Kramer, W. L. and Mulla, M. S. (1982) Ovipositional repellency of fatty acids and their derivatives against Culex and Aedes mosquitoes. Environmental Entomology 11, 223–226.CrossRefGoogle Scholar
Jbilou, R., Amri, H., Bouayad, N., Ghailani, N., Ennabili, A. and Sayah, F. (2008) Insecticidal effects of extracts of seven plant species on larval development, α-amylase activity and offspring production of Tribolium castaneum (Herbst) (Insecta: Coleoptera: Tenebrionidae). Bioresource Technology 99, 959–964.CrossRefGoogle ScholarPubMed
Jeyabalan, D., Arul, N. and Thangamathi, P. (2003) Studies on effects of Pelargonium citrosa leaf extracts on malarial vector, Anopheles stephensi Liston. Bioresource Technology 89, 185–189.CrossRefGoogle ScholarPubMed
Kaufman, D. D. (1976) Bound and conjugated pesticide residues. ACS Symposium Series 29, 1–10.CrossRefGoogle Scholar
Lucas, E. A. and Romoser, W. S. (2001) The energetic costs of diving in Aedes aegypti and Aedes albopictus pupae. Journal of the American Mosquito Control Association 17, 56–60.Google ScholarPubMed
McGeachin, R. L., Willis, T. G., Roulston, E. F. and Lang, C. A. (1972) Variations in α-amylase during the life span of the mosquito. Comparative Biochemistry and Physiology B 43, 185–191.CrossRefGoogle ScholarPubMed
Marinotti, O. and James, A. A. (1990) An α-glucosidase in the salivary glands of the vector mosquito, Aedes aegypti. Insect Biochemistry 20, 619–623.CrossRefGoogle Scholar
Murugan, K., Murugan, P. and Noortheen, A. (2007) Larvicidal and repellent potential of Albizzia amara Boivin and Ocimum basilicum Linn. against dengue vector, Aedes aegypti (Insecta: Diptera: Culicidae). Bioresource Technology 98, 198–201.CrossRefGoogle ScholarPubMed
Peumans, W. J. and Van Damme, E. J. (1995) Lectins as plant defence proteins. Plant Physiology 109, 347–352.CrossRefGoogle Scholar
Ramya, S., Rajasekaran, C., Kalaivani, T., Sundararajan, G. and Jayakumararaj, R. (2008) Biopesticidal effect of leaf extracts of Catharanthus roseus L (G) Don. on the larvae of gram pod borer – Helicoverpa armigera (Hübner). Ethnobotanical Leaflets 12, 1096–1101.Google Scholar
Sadasivam, S. and Manickam, A. (eds) (2005) Biochemical Methods. New Age International Publishers (P) Ltd, New Delhi. 257 pp.Google Scholar
Siddhuraju, P. and Manian, S. (2007) The antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chemistry 105, 950–958.CrossRefGoogle Scholar
Sreerama, Y. N., Neelam, D. A., Sashikala, V. B. and Vishwas, M. P. (2010) Distribution of nutrients and antinutrients in milled fractions of chickpea and horse gram: seed coat phenolics and their distinct modes of enzyme inhibition. Journal of Agriculture and Food Chemistry 58, 4322–4330.CrossRefGoogle ScholarPubMed
Wirth, M. C. (2010) Mosquito resistance to bacterial larvicidal toxins. The Open Toxicology Journal 3, 126–140.CrossRefGoogle Scholar