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Purification of Biotype I population of brown planthopper Nilaparvata lugens (Homoptera: Delphacidae)

Published online by Cambridge University Press:  19 September 2011

Z. R. Khan  and
R. C. Saxena
Z. R. Khan
Affiliation:
International Centre of Insect Physiology and Ecology, P. O. Box 30772, Nairobi, Kenya, based at International Rice Research Institute, P. O. Box 933, Manila, Philippines
R. C. Saxena
Affiliation:
Division of Entomology, International Rice Research Institute, P. O. Box 933, Manila, Philippines
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Abstract

Pure line populations of brown planthopper, Nilaparvata lugens (Ståi), are needed for identifying new genetic sources of resistance. Among the known N. lugens biotypes in the Philippines, Biotype 1 can infest rice cultivare which lack genes for resistance. Biotype 2 can damage cultivars carrying Bph1 resistance gene, in addition to those that are susceptible to Biotype 1. Biotype 3 can infest rice cultivars carrying bph2 resistance gene, in addition to those susceptible to Biotype 1. In the greenhouse Biotypes 1, 2, and 3 are maintained on TN1 (no resistance gene), Mudgo (Bph1 gene) and ASD7 (bph2 gene) rice plants, respectively. Because Biotype 1 population is reared on TN1 plants, susceptible to all the three biotypes, accidental infestations of Biotype 1, Biotype 2 and Biotype 3 cannot be ruled out.

N. lugens biotypes were separated from each other by their differential ingestion and assimilation of food on different susceptible and resistant rice cultivars. A homogeneous population of Biotype 1 of N. lugens was produced from single pairs of males and females selected on the basis of their low food intake on rice cultivars Mudgo and ASD7. The purity of such Biotype 1 population was further demonstrated for three generations based on differential nymphal growth and development and adult longevity and fecundity on resistant Mudgo and ASD7 and on the susceptible TN1 rice cultivars. The technique can also be useful in identifying biotypes of other sucking insect pests.

Résumé

Des populations de lignées pures de la cicadelle brune, Nilaparvata lugens (Stål), sont nécessaires pour l'identification de nouvelles sources génétiques de résistance. Parmi les biotypes connus de N. lugens aux Philippines, le biotype 1 peut infester les cultivars de riz qui ne présentent pas de génes de résistance. Le biotype 2 peut endommager les cultivars qui présentent le gène de résistance Bphl, outre ceux qui sont sensibles au biotype 1. Le biotype 3 peut infester les cultivars de riz qui présentent le gène de résistance bph2 outre ceux qui sont sensibles au biotype 1. En serre, les Biotypes 1, 2 et 3 sont maintenus sur les variétiés TN1 (pas de gènes de résistance), Mudgo (gène Bph1) et ASD7 (gène bph2) respectivement. La population de Biotype 1 étant élevée sur des plantes TN1, sensibles aux trois biotypes, des infestations accidentelles du Biotype 1 par le Biotype 2 et le Biotype 3 ne peuvent entre écartées.

Les biotypes de N. lugens ont été séparés l'un de l'autre en fonction de leur ingestion et de leur assimilation de leur nourriture sur divers cultivars de riz, sensibles et résistants. Une population homogène du Biotype 1 de N. lugens a été obtenue à partir de paires mâle et femelle sélectionnées en fonction de leur faible capacité à se nourrir sur les cultivars Mudgo et ASD7. La pureté de ces populations du Biotype 1 a été démontrée ultérieurement pendant trois générations en se basant sur la croissance et le développement différentiel du stade nymphal et lalongivité et la fécondité du stade adulte sur les cultivars résistants Mudgo et ASD7 et sensible TN1. La technique peut être également utile pour identifier les biotypes d'autres insectes suceurs.

Keywords

Brown planthopperbiotypesinsect resistanceNilaparvata lugensOryza sativa L.riceCicadelle brunebiotypesrésistance aux insectesNilaparvata lugensOryza sativa L.riz
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 11 , Issue 1 , February 1990 , pp. 55 - 62
Copyright
Copyright © ICIPE 1990

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