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Stage- and sex-dependent changes in the lipid profile of Dysdercus koenigii (Heteroptera: Pyrrhocoridae) during development

Published online by Cambridge University Press:  28 February 2007

Archana Mishra ,
A.K. Singh  and
Dinesh Kumar
Archana Mishra
Affiliation:
Department of Zoology, Banaras Hindu University, Varanansi, 221005, India
A.K. Singh
Affiliation:
Department of Zoology, Banaras Hindu University, Varanansi, 221005, India
Dinesh Kumar*
Affiliation:
Department of Zoology, Banaras Hindu University, Varanansi, 221005, India
*
*Email: dkumar@bhu.ac.in;dkumar@banaras.ernet.in
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Abstract

Lipids were extracted from the plasma and gonads of fourth and fifth instars and adults of Dysdercus koenigii (Heteroptera: Pyrrhocoridae). Thin layer chromatography of the extracts revealed fractions of free fatty acids (FFA), monoglycerides (MG), diglycerides (DG), triglycerides (TG), phospholipids (PL), free cholesterol (FC) and esterified cholesterol (EC). There were distinct differences in the lipid profiles within sexes for both tissues during development. Phospholipid from female plasma showed significant fluctuation during development, while that from males remained constant. In female gonads, PL levels increased significantly from the fouth instar to the fifth instar. Monoglycerides were detected only in adult male plasma and adult female gonads, at very low levels. Plasma DG in males was found to increase significantly during development. Maximum gonadal DG was recorded in male fourth instars and female fifth instars. Triglycerides in female plasma declined from the fourth instar to the adult stage. In contrast, TG in gonads increased significantly in the fifth instar of both sexes. Plasma FFA of both sexes fluctuated during development. Free cholesterol was detected only in adult male plasma. Plasma EC increased from the fourth instar to the adult stage in males, and it was highest in gonads of fifth instars of both sexes.

Keywords

Dysdercus koenigiidevelopmentlipid profilethin layer chromatography
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 24 , Issue 3 , September 2004 , pp. 236 - 241
Copyright
Copyright © ICIPE 2004

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References

Beenakkers, A. M. and Gilbert, L. I. (1968) The fatty acid composition of fat body and haemolymph in Hyalophora cecropia and its relation to lipid release. J. Insect Physiol. 14, 481–494.CrossRefGoogle ScholarPubMed
Beenakkers, A. M., Vander-Horst, D. J., Van Marrewijk, W. J. A. (1985) Insect lipids and lipoproteins and their role in physiological processes. Prog. Lipid Res. 24, 19–67.CrossRefGoogle ScholarPubMed
Casida, J. E., Beck, S. D. and Cole, M. J. (1957) Sterol metabolism in the American cockroach. J. Biol. Chem. 224, 365–371.CrossRefGoogle Scholar
Chapman, D. (1975) Lipid dynamics in cell membranes, pp. 13–120.In Cell Membranes: Biochemistry, Cell Biology and Pathology. (Edited by Weissmann, G., Clairborne, R.) (eds) H.P. Publishing, New York.Google Scholar
Chino, H. (1985) Lipid transport: biochemistry of haemolymph lipophorin, pp. 115–136. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (Edited by Kerkut, G. A., Gilbert, L. I.) Pergamon Press, Oxford.Google Scholar
Cmelik, S. H. W. (1969) The neutral lipids from various organs of the termite Macrotermes goliath. J. Insect Physiol. 15, 839–849.CrossRefGoogle Scholar
Dutkowski, A., Ziajka, B. (1970) Sexual dimorphism in the content of lipid in fat body of Galleria melonella L. (Lepidoptera) and utilization of these lipids by developing oocytes for vitellogenesis. Zool. Polon. 20, 55–70.Google Scholar
Fast, P. G. (1964) Insect lipids: a review. Mem. Ent. Soc. Can. 37, 1–50.Google Scholar
Folch, J., Lees, M., Sloane-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509.CrossRefGoogle ScholarPubMed
Freeman, C. P., West, D. (1966) Complete separation of lipids classes on a single thin layer plate. J. Lipid Res. 7, 324–327.CrossRefGoogle ScholarPubMed
Fulton, R. A. and Romney, V. E. (1940) The chloroform soluble components of beet leafhoppers as an indication of the distance they move in spring. J. Agric. Res. 61, 737–743.Google Scholar
Gilbert, L. I. (1967) Lipid metabolism and functions in insects, pp. 69–211. In Advances in Insect Physiology. (Edited by Beament, J. W. L. and Treherne, J. E., Wigglesworth, V. B.). vol 4. Academic Press, London and New York.Google Scholar
Hardie, J. and Lees, A. D. (1985) Endocrine control of polymorphism and polyphenism, pp. 441–490. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (Edited by Kerkut, G. A., Gilbert, L. I.) vol 8. Pergamon Press, Oxford.Google Scholar
Kerkut, G. A. and Gilbert, L. I. (1985) Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol 10. Pergamon Press, Oxford.Google Scholar
Kinsella, J. E. (1966) General metabolism of the hexapod embryo with particular reference to lipids. Comp. Biochem. Physiol. 19, 291–304.CrossRefGoogle ScholarPubMed
Koolman, J. (1989) Ecdysone: From Chemistry to Mode of Action. George Thieme Verlag, Stuttgart, New York.Google Scholar
Lal, B. and Singh, T. P. (1987) Changes in tissue lipid levels in the freshwater catfish Clarias batrachus associated with the reproductive cycle. Fish Physiol. Biochem. 3, 191–201.CrossRefGoogle Scholar
Lee, R. F., Polthemus, J. T., Cheng, L. (1975) Lipids of the water strider Geris remigi Say (Hemiptera: Gerridae) seasonal and developmental variations. Comp. Biochem. Physiol. 51, 451–456.Google Scholar
Marzo, A., Ghirarch, P., Serdini, D., Meroni, D. (1971) Simplified measurement of monoglycerides, diglycerids, triglycerides and fatty acids in biological samples. Clin. Chem. 17, 145–147.CrossRefGoogle Scholar
Nagl, J. F. (1980) Theory of the main lipid bilayer phase transition. Ann Rev. Phys. Chem. 31, 157–195.CrossRefGoogle Scholar
Nakasone, S., Ito, T. (1967) Fatty acid composition of the silkworm Bombyx mori. J. Insect Physiol. 13, 1237–1246.CrossRefGoogle Scholar
Robbins, W. E. (1963) Studies on the utilization, metabolism and function of sterols in the house fly, Musca domestica, 269–280. In Radiation and Radio Isotopes Applied to Insects of Agricultural Importance IAEA, Vienna.Google Scholar
Rockstein, M. (1978) Biochemistry of Insects. Academic Press, New York, London.Google Scholar
Rup, P. J., Gill, R. K. and Kalra, P. K. (1992) Phospholipid during the development of phasephenism in Lipaphis erysimi (Kalt.) (Hemiptera: Aphididae). Entomon 17, 29–34.Google Scholar
Ryan, R. O. (1990) Dynamics in insect lipophorin metabolism. J. Lipid Res. 31, 1725–1739.CrossRefGoogle ScholarPubMed
Scoggin, J. K. and Tauber, O. E. (1950) Survey of literature on insect lipids. Iowa State College J. Sci. 25, 99–124.Google Scholar
Shapiro, J. P., Law, J. H. and Wells, M. A. (1988) Lipid transport in insects. Annu. Rev. Entomol. 33, 297–318.CrossRefGoogle ScholarPubMed
Shelby, K. S. and Chippendale, G. M. (1990) In vitro synthesis and secretion of lipophorin by the fat body of nondiapause and prediapause larvae of the southwestern corn borer Diatraea grandiosella. Insect Biochem. 20, 517–522.CrossRefGoogle Scholar
Sidhu, D. S., Kumar, N. and Dhillon, S. S. (1984) Phospholipid during the developmental stages of Athalia proxmia (A. Lugens) (Hymenoptera: Tenthredinidae). Acta Entomol. Bohemia 81, 411–415.Google Scholar
Singh, A. K. and Singh, T. P. (1979) Seasonal fluctuation in total lipid and cholesterol content in ovary, liver and blood serum in relation to annual sexual cycle in Heteropneustes fossilis (Bloch). Endocrinologie 73, 47–54.Google Scholar
Thomas, K. K. (1974) Lipid composition of the fat body and haemolymph and its relation to lipid release in Oncopeltus fasciatus. J. Insect Physiol. 20, 845–858.CrossRefGoogle ScholarPubMed
Turunen, S. (1975) Absorption and transport of dietary lipid in Pieris brassicae. J. Insect Physiol. 21, 1521–1529.CrossRefGoogle Scholar
Vander-Horst, D. J. (1990) Lipid transport function of lipoproteins in flying insects. Biochem. Biophys. Acta 1047, 195–211.CrossRefGoogle Scholar
Venkatesh, K., Lenz, C. J., Bergman, D. K. and Chippendale, G. M. (1987) Synthesis and release of lipophorin in larvae of the south western corn borer Diatraea grandiosella; an in vitro study. Insect Biochem. 17, 1173–1180.CrossRefGoogle Scholar
Venugopal, K. J., Kumar, D. (1999) Vitellins and vitelllogensis of Dysdercus koenigii (Heteroptera: Pyrrhocoridae) – identification, purification and temporal pattern. Comp. Biochem. Physiol. B. 124, 215–223.CrossRefGoogle ScholarPubMed
Venugopal, K. J., Kumar, D. and Singh, A. K. (1994) Developmental studies on protein from haemolymph, fat body and ovary of the phytophagous bug Dysdercus koenigii (Heteroptera: Pyrrhocoridae). Biochem. Archi. 10, 297–302.Google Scholar
Wang, C. M. and Patton, R. L. (1969) Lipids in the haemolymph of the cricket Acheta domesticus. J. Insect Physiol. 15, 851–860.CrossRefGoogle Scholar
Weers, P. M. M., Vander-Horst, D. J., Van Marrewijk, W. J. A., Vanden Eijnden, M., Van Doorn, J. M., Beenakkers, A.M. (1992) Biosynthesis and secretion of insect lipoproteins. J. Lipid Res. 33, 485–491.CrossRefGoogle Scholar
Wlodawer, P., Wisniewsak, A. (1965) Lipids in the haemolymph of the wax moth larvae during starvation. J. Insect Physiol. 11, 11–20.CrossRefGoogle Scholar
Zaidi, A. S. and Ansari, H. J. (1985) Phospholipid concentration in the fat body and gonad of the red cotton bug Dysdercus cingulatus. Fabr. (Heteroptera: Pyrrhocoridae) in relation to growth, metamorphosis and reproduction. Bull. Zool. 52, 305–308.CrossRefGoogle Scholar