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Synthesis and transport of RNA in the salivary glands of some bugs (Heteroptera)

Published online by Cambridge University Press:  19 September 2011

P. R. Yadav  and
Dinesh Kumar
P. R. Yadav
Affiliation:
Silkworm Seed Technology Laboratory, Kodathi, Carmelram post, Bangalore-560035, India
Dinesh Kumar
Affiliation:
Department of Zoology, Banaras Hindu University, Varanasi-221005, India
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Abstract

Autoradiographic studies using 3H uridine in the salivary glands of three bugs, Lethocerus indicus, Laccotrephes rubra and Antilochus coqueberti show that the nuclei play an important role in contributing RNA. The labelled materials are seen to move into the lumen from the epithelia. RNA shows a tendency to accumulate in the lumen with ascending incubation periods. This behaviour in conjunction with its nucleolar genesis indicates its nature as rRNA. It is also believed that RNA might conjugate with some of the protein available in the lumen to produce RNP.

Résumé

Des etudes autoradiographiques des glandes salivaires de 3 espèces de punaises, Lethocerus indicus, Laccotrephes rubra et Antilochus coqueberti, a l'aide de l'uridine radioactive (3H) montrent que les nuclei jouent un rôle primordial dans la synthèse de TARN. Le matériel radioactif semble se déplacer de l'épithélium vers la lumière. L' ARN montrerait une tendance à s'accumuler dans la lumière au fur et à mesure que se prolongent les périodes d'incubation. Ce fait, en conjunction avec l'origine nucléique de l'ARN confirme que l'ARN en question ici est d'origine ribosomal. On suppose aussi que l'ARN se combinerait avec certaines protéines présentes dans la lumière pour former des PRN.

Keywords

Salivary glandbugLethocerus indicusLaccotrephes rubraAntilochus coquebertiRNA metabolismautoradiographyd3HuridineGlande salivairepunaiseLethocerus indicusLaccotrephes rubraAntilochus coquebertimétabolisme de l'ARNautoradiographieuridine 3H
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 14 , Issue 4 , August 1993 , pp. 411 - 418
Copyright
Copyright © ICIPE 1993

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References

REFERENCES

Akai, H. and Kobayashi, M. (1965a) Site of the fibroin formation in the silk glands of Bombyx mori. Nature 206, 529.CrossRefGoogle Scholar
Akai, H. and Kobayashi, M. (1965b) Incorporation of the labelled thymidine into the silk glands of the silkworm. Nature 206, 847–848.CrossRefGoogle ScholarPubMed
Beams, H. and Sekhon, S. (1966) Morphological studies on the secretion in the silk glands of the caddish fly larvae, Platyphalyx designatus. Z. Zellforsh 72, 408–414.CrossRefGoogle Scholar
Berry, S. J. (1985) RNA synthesis and storage during insect oogenesis. In Developmental Biology. A Comprehensive Synthesis Vol. 1 Oogenesis (Edited by Browder, L. W.), Plenum Press, N. York.Google Scholar
Bier, K. (1963) Synthese, interzellularev Transport und Abbu von Ribonucleinsaure in Ovar der Stubenfliege Musca domestica. J. Cell Biol. 16, 436–440.CrossRefGoogle Scholar
Capco, D. and Jeffery, W., (1979) Origin and special distribution of maternal mRNA during oogenesis of an insect Oncopeltus fasciatus. J. Cell Sci. 39, 63–76.CrossRefGoogle Scholar
Cave, M. D. (1973) Synthesis and characterisation of amplified DNA oocytes of the house cricket, Achaeta domesticus (Orthoptera: Gyrillaidae). Chromosoma 42, 1–22.CrossRefGoogle Scholar
Gundevia, H. S. (1985) Autoradiographic studies on RNA synthesis and transport in the ovary of Hydrophylus olivaceus (Coleoptera). Experientia 41, 383–385.CrossRefGoogle Scholar
Gundevia, H. S. (1989) Studies on the RNA synthesis and transport in the corpora allata of Hydrophylus olivaceus Fabr. (Coleoptera). Zool. Jb. Physiol. 93, 347–352.Google Scholar
Haridass, E. T., and Ananthakrishnan, T. N. (1981) Functional morphology of the salivary system in some Reduviidae (Insecta:Heteroptera), Proc. Indian Acad. Sci. (Anim. Sci.) 90, 145–160.CrossRefGoogle Scholar
Jacob, J. (1967) An electron microscopic autoradiographic study of the site of initial synthesis of RNA in the nucleolus of Smittia. Exp. Cell Res. 48, 276–282.CrossRefGoogle ScholarPubMed
Jebanesan, A., Thatheyus, A. J. and Selvanayagam, M. (1988) Mosquito control by aquatic hemipterans in river Cooum, Madras. Bicovas 1, 203–211.Google Scholar
Jayachandra, C. M. and Venkatesan, P. (1988) Predatory efficiency of giant water bug, Lethocerus indicus Lep & Serv (Hemiptera:Be-lostomatidae: Lethocerinae). Bicovas 1, 13–26.Google Scholar
King, R. C. and Burnett, R. G. (1959) Autoradiographic studies of uptake of tritiated glycine, thymidine and uridine by fruit fly ovaries. Science 19, 1674–1675.CrossRefGoogle Scholar
Kumar, D. (1978) Histophysiological studies of the salivary apparatus of Indian Hemiptera: Morphological, histological, histochemical, autoradiographic and electron microscopic investigations. Ph. D. thesis, BHU, Varanasi, India.Google Scholar
Miles, P. W. (1972) The saliva of Hemiptera. Adv. Insect Physiol. 9, 183–225.CrossRefGoogle Scholar
MiMer, N. C. E. (1971) The Biology of the Heteroptera. Second (revised) edition, Hampton (England). E. W. Classey.Google Scholar
Muhlach, W. E. and Schwalm, F. E. (1977) Utilisation of uridine in developing ovarioles of the kelp fly, Coelopa. J. Insect Physiol. 23, 931–937.CrossRefGoogle Scholar
Nummelin, M. (1988) Water striders (Het: Gerridae) as predators of hatching mosquitoes. Bicovas 1, 121–125.Google Scholar
Ramamurty, P. S. (1963) Uberdie Horkunft der Ribonulkeinsaure in den wachsenden Eizellen der Skorpionsfliege, Panorpa communis. Naturwischft 50, 383–384.CrossRefGoogle Scholar
Ray, A. (1979) Autoradiographic studies on RNA synthesis in trophophocytes and germinal vesicles of Menochilus sexmaculatus (Coleoptera: Coccinellidae). Entomon 4, 1–5.Google Scholar
Ray, A. and Ramamurty, P. S. (1979) Sources of RNA supply to the oocytes in Crynodes peregrinus F. (Coleoptera: Chrysomelidae) Int. J. Insect Morphol. Embryol. 8, 113–122.CrossRefGoogle Scholar
Sirlin, J. L. and Jacob, J. (1964) Sequential and reversible inhibition of synthesis of RNA in the nucleolus and chromosomes. Effect of benzamide and substituted benzamiadozoles on dipteran salivary glands. Nature 204, 545–547.CrossRefGoogle ScholarPubMed
Takeda, N. (1976) Activatory mechanisms of the prothoracic glands of Monema flavescens (Lepidoptera) with special reference to the secretion of ecdysone. Biol. Bull. 150, 500–521.CrossRefGoogle Scholar
Wiley, M. J. and Lai-Fook, J. (1974) Studies on the silk glands of Calpodesethlius Stall. (Lepidoptera:Hersperudae). J. Morphol. 144, 297–322.CrossRefGoogle Scholar
Yadav, P. R. (1988) Studies on the salivary apparatus and saliva of some Hemiptera. Ph. D. thesis, BHU, Varanasi, India.Google Scholar
Yadav, P. R. (1990) Morphological, histological and histochemical observation on the salivary glands of Ranatra filiformis Fabrr (Heteroptera). J. Zool. Res. 3, 89–99.Google Scholar
Yadav, P. R. (1992) Protein synthesis in the salivary glands of some bugs: An autoradiographic study of 3H glycine incorporation. Insect Sci. Applic. 13, 49–58.Google Scholar
Zalokar, M. (1960) Sites of ribonucleic acid and protein synthesis in Drosophila. Exp. Cell Res. 19, 184–196.CrossRefGoogle ScholarPubMed