Hostname: page-component-848d4c4894-m9kch Total loading time: 0 Render date: 2024-06-04T15:30:10.126Z Has data issue: false hasContentIssue false
  • English
  • Français

Lysine and nitrogen measurement by infra-red reflectance analysis as an aid to barley breeding

Published online by Cambridge University Press:  27 March 2009

A. A. Gill ,
Carol Starr  and
D. B. Smith
A. A. Gill
Affiliation:
Chemistry Department, Plant Breeding Institute, Trumpington, Cambridge GB2 2LQ
Carol Starr
Affiliation:
Chemistry Department, Plant Breeding Institute, Trumpington, Cambridge GB2 2LQ
D. B. Smith
Affiliation:
Chemistry Department, Plant Breeding Institute, Trumpington, Cambridge GB2 2LQ
Get access Rights & Permissions [Opens in a new window]

Summary

The feasibility of measuring lysine and protein nitrogen in barley using an infra-red reflectance analyser was examined. This is a very rapid technique, requiring no manipulative skills, and measuring several components simultaneously using milled grains. The results show that this instrument is sensitive to changes in lysine and protein nitrogen and can be calibrated to measure these components to a high degree of accuracy. It is shown that the gross morphological and biochemical abnormalities of high lysine mutants do not affect these measurements, indicating that this method is suitable for the rapid screening of material from breeding programmes designed to improve the lysine content of barley proteins.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 93 , Issue 3 , December 1979 , pp. 727 - 733
Copyright
Copyright © Cambridge University Press 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allison, M. J., Cowe, I. A. & Mohale, R. (1978). The use of infra red reflectance for the rapid estimation of the soluble β-glucan content of barley. Journal of the Institute of Brewing 84, 153–155.CrossRefGoogle Scholar
Association of Official Analytical Chemists (1975). Official Methods of Analysis, 12th edn., p. 232. Washington, DC: A.O.A.C.Google Scholar
Carpenter, K. J. (1958). Chemical methods of evaluating protein quality. Proceedings of the Nutrition Society 17, 91–100.CrossRefGoogle Scholar
Carpenter, K. J. & Ellinger, G. M. (1955). Protein quality and ‘available lysine’ in animal products. Poultry Science 34, 1451–1452.CrossRefGoogle Scholar
Christoffers, D., Kaul, A. K. & Georgi, B. (1974). Determination of lysine in grain-meal samples by dansylation. Qenetika, Yugoslavia 6, 89–94.Google Scholar
Dévényi, T., Pongor, S., Váradi, A. & Axmann, H. (1978). Video-densitometry: A rapid method for analysis of amino acids suitable for large scale screening programmes. In Seed Improvement by Nuclear Techniques, IAEA Vienna, pp. 349–354.Google Scholar
Doll, H. (1975). Genetic studies of high lysine barley mutants. In Barley Genetics. Proceedings of the Third International Barley Oenetics Symposium, Garching, pp. 542–546.Google Scholar
Hart, H. V. (1978). The InfraAlyser - Further information for determination of protein and moisture in wheat. FMBRA Bulletin, no. 5, pp. 173–175.Google Scholar
Hymowitz, Z., Dudley, J. W., Collins, F. I. & Brown, C. M. (1974). Estimations of protein and oil concentration in corn, soybean and oat seed by near infra red light reflectance. Crop Science 14, 713–715.CrossRefGoogle Scholar
Jenkins, G., Rhodes, A. P., Gill, A. A. & Hanson, P. R. (1979). The effect of irrigation and nitrogen supply on the yield and quality of proteins in high lysine barleys. Journal of the Science of Food and Agriculture (in the Press).Google Scholar
Kreis, K. (1978). Starch and free sugars during kernel development of Bomi barley and its high lysine mutant 1508. In New Approaches to Breeding for Improved Plant Protein, IAEA Vienna, pp. 115–120.Google Scholar
Mossberg, R. (1969). Evaluation of protein quality and quantity by dye binding capacity: a tool in plant breeding. In New Approaches to Breeding for Improved Plant Protein, IAEA Vienna, pp. 151–160.Google Scholar
Niemann, E. G., Christoffers, D., Georgi, B., Kaestner, H. W., Mohyuddin, M. & Sharma, T. R. (1978). Screening methods for protein amino acids and nutritional value in crops and processed food. In Seed Protein Improvement by Nuclear Techniques, IAEA Vienna, pp. 331–345.Google Scholar
Operation Manual for the Technicon Infraalyser Plus (1976). Technicon publication no. Ta6–0350–00.Google Scholar
Pomeranz, Y. & Moore, R. B. (1975). Reliability of several methods for protein determination in wheat. Bakers Digest 50, 44–48.Google Scholar
Rhodes, A. P. (1975). A comparison of two rapid screening methods for the selection of high lysine barleys. Journal of the Science of Food and Agriculture 26, 1703–1710.CrossRefGoogle Scholar
Rubenthaler, G. L. & Bruinsma, B. L. (1978). Lysine estimation in cereals by near infrared reflectance. Crop Science 18, 1039–1042.CrossRefGoogle Scholar
Starr, C. & Smith, D. B. (1978). A semi-micro dryblock and automated analyser technique suitable for determining protein nitrogen in plant material. Journal of Agricultural Science, Cambridge 91, 639–644.CrossRefGoogle Scholar
Watson, C. A., Shuey, W. C., Blanasik, O. J. & Dick, J. W. (1977). Effect of wheat class on near infrared reflectance. Cereal Chemistry 54, 1264–1269.Google Scholar
Whitmore, E. T. & Smith, D. B. (1969). A modified technique for the automatic determination of Llysine and amino nitrogen in cereal hydrolysates. Technicon Symposium: Proceedings of the Automation in Analytical Chemistry, pp. 193–195.Google Scholar
Williams, P. C. (1975). Application of near infra red spectroscopy to analysis of cereal grains and oilseeds. Cereal Chemistry 52, 561–576.Google Scholar