Hostname: page-component-76fb5796d-x4r87 Total loading time: 0 Render date: 2024-04-30T04:21:54.228Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on nitrogen fixation by antibiotic-resistant mutants of Azospirillum brasilense and their interaction with cheena (Panicum miliaceum L.) genotypes in calcareous soil

Published online by Cambridge University Press:  27 March 2009

R. Rai
R. Rai
Affiliation:
Rajendra Agricultural University, Dholi Campus, Dholi, Muzqffarpur, Bihar, India – 843121
Get access Rights & Permissions [Opens in a new window]

Summary

Azospirillum brasilense was treated with nitrosoguanidine and five antibiotic-resistant mutant strains isolated. Variations in growth, N2-fixation, ultraviolet-dark survival and level of antibiotic resistance were found between the mutant strains. Mutant strains STR 112 and KR 2051 showed maximum nitrogenase activity, glutamine synthetase activity and hydrogenase activity (H2 uptake) at 32 °C and 40 °C respectively. Inoculation of cheena genotypes with A. brasilense and its mutants led to significant increase in associative nitrogen fixation, dry weight of roots and grain yield compared with the uninoculated control, with significant strains × genotypes interactions in calcareous soil. It was also noted that under laboratory conditions Azospirillum inoculation may have produced its response by hormonal means and/or associative N2-fixation.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 105 , Issue 2 , October 1985 , pp. 261 - 270
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Albrecht, S. L., Okon, Y. & Burris, R. H. (1977). Effect of light and temperature on the association between Zea mays and Spirillum lipoferum. Plant Physiology 60, 528–531.CrossRefGoogle ScholarPubMed
Baldani, V. L. D. & Dobereiner, J. (1980). Hostplant specificity in the infection of cereals with Azospirillum spp. Soil Biology and Biochemistry 12, 433–439.CrossRefGoogle Scholar
Cohen, E., Okon, Y., Kigel, J., Nur, I. & Henis, Y. (1980). Increase in dry weight and total content in Zea mays and Setaria italica associated with nitrogenfixing Azospirillum spp. Plant Physiology 66, 746–749.CrossRefGoogle ScholarPubMed
Dart, P. J., Day, J., Islam, R. & Dobereiner, J. (1976). Symbiosis in tropical grain legumes: some effects of temperature and the composition of the rooting medium. In Symbiotic Nitrogen Fixation in Plants (ed. Nutman, P. S.), pp. 361–383. Cambridge: Cambridge University Press.Google Scholar
Dart, P. J. & Wani, S. P. (1982). Non-symbiotic nitrogen fixation and soil fertility. In Non-symbiotic Nitrogen Fixation and Organic Matter in the Tropics. 12th International Congress of Soil Science, 02 8–16. 1982, pp. 1–27, New Delhi.Google Scholar
De-Polli, H., Boyer, C. D. & Neyra, C. A. (1982). Nitrogenase activity associated with roots and stems of field grown corn (Zea mays L.) plants. Plant Physiology 70, 1609–1613.CrossRefGoogle ScholarPubMed
Dobereiner, J. (1981). Emerging technology based on BNF by associative N2-fixing organisms. Paper presented at International Workshop on Biological Nitrogen Fixation Technology for Tropical Agriculture, 9–1303 1981. Cali, Colombia.Google Scholar
Dobereiner, J. & Baldani, V. (1979). Selective infection of maize roots by streptomycin-resistant Azospirillum lipoferum and other bacteria. Canadian Journal of Microbiology 25, 1264–1269.CrossRefGoogle ScholarPubMed
Dobereiner, J. & Day, J. M. (1976). Associative symbiosis in tropical grasses: characterization of microorganisms, and dinitrogen fixing sites. In Proceedings of the 1st International Symposium on N2-Fixation (ed. Newton, W. E. and Nyman, C. J.), pp. 518–538. Pullman: Washington State University Press.Google Scholar
Dobereiner, J., Marriel, J. E. & Nery, M. (1976). Ecological distribution of Spirillum lipoferum Beijerinck. Canadian Journal of Microbiology 22, 1464–1473.CrossRefGoogle ScholarPubMed
Emerich, D. W., Ruiz-Argueso, T., Ching, N. & Evans, H. J. (1979). Hydrogen-dependent nitrogenase activity and ATP formation in Bhizobium japonicum bactoroids. Journal of Bacteriology 137, 153–160.CrossRefGoogle Scholar
Gauthier, D. & Elmerich, C. (1977). Relationship between glutamine synthetase and nitrogenase in Spirillum lipoferum. FEMS Microbiology Letter 2, 101–104.CrossRefGoogle Scholar
Gibson, A. A. (1963). Physical environment and nitrogen fixation. 1. The effect of root temperature on recently nodulated Trifolium subterraneum. L. Australian Journal of Biological Science 16, 28–42.CrossRefGoogle Scholar
Kleckner, N., Roth, J. & Botstein, D. (1977). Genetic engineering in vivo using translocatable drug resistance elements: new methods in bacterial genetics. Journal of Molecular Biology 116, 125–159.CrossRefGoogle ScholarPubMed
Lakshmi-Kumari, M., Kavimandan, S. K. & Subba Rao, N. S. (1976). Occurrence of nitrogen fixing Spirillum in roots of rice, sorghum, maize and other plants. Indian Journal of Experimental Biology 14, 638–649.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Neyra, C. A. & Dobereiner, J. (1977). Nitrogen fixation in grasses. Advances in Agronomy 29, 1–38.CrossRefGoogle Scholar
Norris, D. O. & Date, A. R. (1976). Legume bacteriology. Commonwealth Bureau of Pastures and Field Crops Bulletin No. 115, p. 134. England.Google Scholar
Okon, Y., Albrecht, S. L. & Burris, R. H. (1977). Methods for growing Spirillum lipoferum and counting it in pure culture and in association with plants. Applied Environmental Microbiology 33, 85–88.CrossRefGoogle ScholarPubMed
Rai, R. (1983). Efficiency of associative N2-fixation by streptomycin-resistant mutants of Azospirillum brasilense with genotypes of chick pea Rhizobium strains. Journal of Agricultural Science. Cambridge 100, 75–80.CrossRefGoogle Scholar
Rai, R. & Prasad, V. (1983). Studies on compatibility of nitrogen fixation by high-temperature-adapted Rhizobium strains and Vigna radiata genotypes at two moisture levels in calcareous soil. Journal of Agricultural Science, Cambridge 101, 377–381.CrossRefGoogle Scholar
Rai, R., Prasad, V. & Shukla, I. C. (1984). Interaction between finger millet (Eleusine coracana) genotypes and drug-resistant mutants of Azospirillum brasilensein calcareous soil. Journal of Agricultural Science, Cambridge 102, 521–529.CrossRefGoogle Scholar
Roberts, G. P. (1981). Genetics and regulation of nitrogen fixation. Annual Review of Microbiology 35, 207–235.CrossRefGoogle ScholarPubMed
Schubert, K. R. & Evans, H. J. (1976). Hydrogen evolution: a major factor affecting the efficiency of nitrogen fixation in nodulated symbionts. Proceedings of the National Academy of Sciences, U.S.A. 73, 1207–1211.CrossRefGoogle Scholar
Subba Rao, N. S. (1979). Crop response to miorobial inoculation. In Recent Advances in Biological Nitrogen Fixation (ed. Rao, N. S. Subba), pp. 406–420. New Delhi: Oxford and IBH Publishing Company.Google Scholar
Tien, T. M., Gaskin, M. H. & Hubbell, D. H. (1979). Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet. Applied Environmental Microbiology 37, 1012–1024.CrossRefGoogle ScholarPubMed
Von Bulow, J. F. W. & Dobereiner, J. (1975). Potential of nitrogen fixation in maize genotypes in Brazil. Proceedings of the National Academy of Sciences, U.S.A. 72, 2389–2393.CrossRefGoogle ScholarPubMed