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Studies on the digestibility and utilization of the nitrogen of irradiated rumen bacteria by rats

Published online by Cambridge University Press:  27 March 2009

V. C. Mason  and
R. Palmer
V. C. Mason
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
R. Palmer
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
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Summary

Rumen bacteria were isolated from the rumen contents of eight Friesian cattle that had been fed a barley-soyabean diet. These preparations were freeze-dried, irradiated with 1 Mrad and analysed for various nitrogenous components.

In nitrogen utilization studies involving both young and adult rats it was observed that the ‘true’ digestibility of the bacterial nitrogen was about 80%, while the net protein utilization (NPU) value was approximately 57.

It was concluded that about 20% of the bacterial nitrogen, mainly incorporated in the cell wall, was not absorbed by the rat. On the other hand, about 71% of the absorbed nitrogen was utilized by the growing rat for tissue synthesis.

Irradiation with 1 Mrad killed virtually all the rumen bacteria without adversely influencing the nutritional value of the bacterial protein.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 76 , Issue 3 , June 1971 , pp. 567 - 572
Copyright
Copyright © Cambridge University Press 1971

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References

REFERENCES

Bergen, W. G., Purser, D. B. & Cline, J. H. (1968). Determination of limiting amino acids of rumenisolated microbial proteins fed to rats. J. Dairy Sci. 51, 1698–1700.CrossRefGoogle Scholar
Burton, K. (1956). A study of the conditions and mechanisms of the Diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–23.CrossRefGoogle ScholarPubMed
Eadie, J. M., Hyldgaard-Jensen, J., Mann, S. O., Reid, R. S. & Whitelaw, F. G. (1970). Observations on the microbiology and biochemistry of the rumen in cattle given different quantities of a pelleted barley ration. Br. J. Nutr. 24, 157–77.CrossRefGoogle ScholarPubMed
Johnson, B. C., Hamilton, T. S., Robinson, W. B. & Garey, J. C. (1944). On the mechanism of non-protein nitrogen utilization by ruminants. J. Anim. Sci. 3, 287–98.CrossRefGoogle Scholar
Kay, M. & Macdearmid, A. (1969). The effects of diets containing different levels of crude protein on food intake and growth rate of Friesian bulls. In Proc. Symp. on Meat Production from Entire Male Animals. (ed. Rhodes, R. N.), pp. 63–8. Bristol: Churchill.Google Scholar
Kraan, J. G. & Muir, H. (1957). Determination of glucosamine. Biochem. J. 66, 55.Google Scholar
Luria, S. E. (1960). The bacterial protoplasm: composition and organization. In The Bacteria, (ed. Gunsalus, I. C. and Stanier, R. Y.), pp. 1–34. New York and London: Academic Press.Google Scholar
McNaught, M. L., Smith, J. A. B., Henry, K. M. & Kon, S. K. (1950). The utilization of non-protein nitrogen in the bovine rumen. 5. The isolation and nutritive value of a preparation of dried rumen bacteria. Biochem. J. 46, 32–6.CrossRefGoogle ScholarPubMed
McNaught, M. L., Owen, E. C., Henry, K. M. & Kon, S. K. (1954). The utilization of non-protein nitrogen in the bovine rumen. 8. The nutritive value of the proteins of preparations of dried rumen bacteria, rumen protozoa and brewer's yeast for rats. Biochem. J. 56, 151–6.CrossRefGoogle Scholar
Mason, V. C. (1969). Some observations on the distribution and origin of nitrogen in sheep faeces. J. agric. Sci., Camb. 73, 99–111.CrossRefGoogle Scholar
Mason, V. C. (1971). Some preliminary observations on the nature of factors influencing the excretion of nondietary faecal nitrogen by ruminant animals. J. agric. Sci., Camb. 76, 157–66.CrossRefGoogle Scholar
Miller, D. S. & Bender, A. E. (1955). The determination of the net utilization of proteins by a shortened method. Br. J. Nutr. 9, 382–8.CrossRefGoogle ScholarPubMed
Purser, D. B. & Buechler, S. M. (1966). Amino acid composition of rumen organisms. J. Dairy Sci. 49, 81–4.CrossRefGoogle ScholarPubMed
Reed, F. M., Moir, R. J. & Underwood, E. J. (1949). Rumen flora studies in the sheep. 1. The nutritive value of rumen bacterial protein. Aust. J. Sci. Res. B 2, 304–17.Google Scholar
Rondle, C. J. M. & Morgan, W. T. J. (1955). The determination of glucosamine and galactosamine. Biochem. J. 61, 586–9.CrossRefGoogle ScholarPubMed
Synge, R. L. M. (1951). Non-protein nitrogeneous constituents of ryegrass: Ionophoretic fractionation and isolation of a ‘bound amino-acid’ fraction. Biochem. J 642–50.Google Scholar
Van Slyke, D. D., Dillon, R. T., MacFadyen, D. A. & Hamilton, P. (1941). Gasometric determination of carboxyl groups in free amino acids. J. biol. Chem. 141, 627–69.CrossRefGoogle Scholar