Hostname: page-component-76fb5796d-9pm4c Total loading time: 0 Render date: 2024-04-30T02:22:36.472Z Has data issue: false hasContentIssue false
  • English
  • Français

The effects of pregnancy, lactation and involution on the metabolism of glucose and acetate by rat liver tissue

Published online by Cambridge University Press:  01 June 2009

R. W. Smith
R. W. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
Get access Rights & Permissions [Opens in a new window]

Summary

The incorporation of 14C from [1-14C] and [6-14C]glucose and [2-14C]acetate into CO2 and fatty acids by rat liver slices was measured at intervals during pregnancy, lactation and involution.

During late pregnancy, the rates of oxidation of the C-1 and C-6 atoms of glucose were respectively 65 and 40 % higher than those for unmated animals. These increases were maintained during lactation, but the highest values were observed 3 days after weaning. Pregnancy and lactation had little effect on the oxidation of [2-14C]acetate.

The incorporation of14C from all 3 labelled substrates into fatty acids was increased by a factor of 3–4 during late pregnancy. There were further increases during lactation, and 3 days after weaning the values were as much as 10 times as high as those for unmated animals.

The incorporation of both [14C]glucose and [14C]acetate into cholesterol was increased by a factor of 6–7 during lactation.

The activities of the enzymes glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, ATP citrate lyase and acetyl-CoA carboxylase were also increased during lactation and involution.

The similarity between the changes summarized above and those brought about by changes in the pattern of food intake is discussed, and the idea that fatty acids synthesized from non-lipid precursors in the liver may make some contribution to the formation of milk fat is also considered.

Type
Research Article
Information
Journal of Dairy Research , Volume 40 , Issue 3 , October 1973 , pp. 339 - 351
Copyright
Copyright © Proprietors of Journal of Dairy Research 1973

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abraham, S. & Chaikoff, I. L. (1959). Journal of Biological Chemistry 234, 2246.CrossRefGoogle Scholar
Bartley, J. C., Abraham, S. & Chaikoff, I. L. (1966). Proceedings of the Society for Experimental Biology and Medicine 123, 670.CrossRefGoogle Scholar
Bosch, V. & Camejo, G. (1967). Journal of Lipid Research 8, 138.CrossRefGoogle Scholar
Brown, W. D. (1959). Australian Journal of Experimental Biology and Medical Science 37, 523.CrossRefGoogle Scholar
Dannenburg, W. N., Burt, R. L. & Leake, N. H. (1964). Proceedings of the Society for Experimental Biology and Medicine 115, 604.Google Scholar
Duncan, D. B. (1955). Biometrics 11, 1.CrossRefGoogle Scholar
Fain, J. N. & Scow, R. O. (1966). American Journal of Physiology 210, 19.CrossRefGoogle Scholar
Fell, B. F., Smith, K. A. & Campbell, R. M. (1963). Journal of Pathology and Bacteriology 85, 179.CrossRefGoogle Scholar
Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957). Journal of Biological Chemistry 226, 497.CrossRefGoogle Scholar
Glascock, R. F., Welch, V. A., Bishop, C., Davies, T., Wright, E. W. & Noble, R. C. (1966). Bio chemical Journal 98, 149.Google Scholar
Glock, G. E. & McLean, P. (1953). Biochemical Journal 55, 400.CrossRefGoogle Scholar
Hagerman, D. D. (1962). Endocrinology 70, 88.CrossRefGoogle Scholar
Jones, E. A. (1967). Biochemical Journal 103, 420.CrossRefGoogle Scholar
Katz, J. & Wood, H. G. (1960). Journal of Biological Chemistry 235, 2165.CrossRefGoogle Scholar
Kumaresan, P. & Turner, C. W. (1968). Proceedings of the Society for Experimental Biology and Medicine 129, 957.CrossRefGoogle Scholar
McKay, D. G. & Kaunitz, H. (1963). Metabolism 12, 990.Google Scholar
McLean, P. (1958). Biochimica et Biophysica Acta 30, 303.CrossRefGoogle Scholar
Masoro, E. J. (1962). Journal of Lipid Research 3, 149.CrossRefGoogle Scholar
Otway, S. & Robinson, D. S. (1968). Biochemical Journal 106, 677.CrossRefGoogle Scholar
Popják, G. (1954). Cold Spring Harbor Symposia on Quantitative Biology 19, 200.CrossRefGoogle Scholar
Robinson, D. S. (1970). In Comprehensive Biochemistry 18, 51. (Eds Florkin, M. and Stotz, E. H..) Amsterdam: Elsevier.Google Scholar
Schwenk, E. & Joachim, E. (1961). Proceedings of the Society for Experimental Biology and Medicine 108, 665.CrossRefGoogle Scholar
Smith, R. W. & Glascock, R. F. (1969). Journal of Dairy Research 36, 455.CrossRefGoogle Scholar
Smith, R. W. & Phillips, S. H. (1969). International Journal of Applied Radiation and Isotopes 20, 553.CrossRefGoogle Scholar
Smith, S., Gagnè, H. T., Pitelka, D. R. & Abraham, S. (1969). Biochemical Journal 115, 807.CrossRefGoogle Scholar
Song, C. S., Rifkind, A. B., Gillette, P. N. & Kappas, A. (1969). American Journal of Obstetrics and Oynecology 105, 813.CrossRefGoogle Scholar
Srere, P. A. (1959). Journal of Biological Chemistry 234, 2544.CrossRefGoogle Scholar
Tepperman, H. M. & Tepperman, J. (1964). Federation Proceedings 23, 73.Google Scholar
Willmer, J. S. (1960). Canadian Journal of Biochemistry and Physiology 38, 1265.CrossRefGoogle Scholar