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Milk proteomic analysis reveals differentially expressed proteins in high-yielding and low-yielding Guanzhong dairy goats at peak lactation
Published online by Cambridge University Press: 28 February 2024
Abstract
The aim of this experiment was to investigate the differential proteomic characteristics of milk from high- and low-yielding Guanzhong dairy goats during the peak lactation period under the same feeding conditions. Nine Guanzhong dairy goats with high yield (H: 3.5 ± 0.17 kg/d) and nine with low yield (L:1.2 ± 0.25 kg/d) were selected for milk proteomic analysis using tandem mass tag technology. A total of 78 differentially expressed proteins were identified. Compared with L, 50 proteins including HK3, HSPB1 and ANXA2 were significantly upregulated in H milk, while 28 proteins including LALBA and XDH were significantly downregulated. Bioinformatics analysis of the differentially expressed proteins showed that galactose metabolism, purine metabolism, glycolysis/gluconeogenesis, MAPK signaling pathway, regulation of actin cytoskeleton and other pathways were closely related to milk yield. HK3, HSPB1, ANXA2, LALBA and XDH were important candidate proteins associated with the milk production characteristics of Guanzhong dairy goats. Our data provide relevant biomarkers and a theoretical basis for improving milk production in Guanzhong dairy goats.
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- Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation
References
Aaron, AM, Edgard, M, Sen, H, Affan, S, Grant, H and Michel, A (2020) PI3 K-dependent Reprogramming of hexokinase isoforms regulates B lymphocyte metabolism. Journal of Immunology 204(151), 30.Google Scholar
Bortolotti, M, Polito, L, Battelli, MG and Bolognesi, A (2021) Xanthine oxidoreductase: one enzyme for multiple physiological tasks. Redox Biology 41, 101882.CrossRefGoogle ScholarPubMed
Bruey, JM, Ducasse, C, Bonniaud, P, Ravagnan, L, Susin, SA, Diaz-Latoud, C, Gurbuxani, S, Arrigo, AP, Kroemer, G and Solary, E (2000) Hsp27 negatively regulates cell death by interacting with cytochrome c. Nature Cell Biology 22, 816–834.Google Scholar
Carroll, AC, Erin, M, Haven, T, Tamara, F, Jessica, S, Deborah, H, Damian, L, Maruf, K, Tali, G, James, DL, Alfred, LF, Habil, Z and Karl, AR (2023) Hspb1 overexpression improves life span and stress resistance in an invertebrate model. The Journals of Gerontology: Series A 77, 268–275.Google Scholar
Chandel, NS (2021) Glycolysis. Cold Spring Harbor Perspectives in Biology 13, a040535.CrossRefGoogle ScholarPubMed
Chang, Z (2014) Main factors affecting the milk yield of Guanzhong milk goats. Anhui Agricultural Science[in China] 42, 442+445.Google Scholar
Chen, K, Hou, C, Xu, L, Peng, H, He, C, Liu, J, Wang, G, Huang, S and Liu, X (2022) HSPB1 regulates autophagy and apoptosis in vascular smooth muscle cells in arteriosclerosis obliterans. Cardiovascular Therapeutics 2022, 3889419.CrossRefGoogle ScholarPubMed
Coscia, A, Orrù, S, Di Nicola, P, Giuliani, F, Varalda, A, Peila, C, Fabris, C, Conti, A and Bertino, E (2012) Detection of cow's milk proteins and minor components in human milk using proteomics techniques. Journal of Maternal-Fetal Medicine 25, 49–51.CrossRefGoogle ScholarPubMed
Cunsolo, V, Saletti, R, Muccilli, V and Foti, S (2010) Characterization of the protein profile of donkey's milk whey fraction. Journal of Mass Spectrometry 42, 1162–1174.CrossRefGoogle Scholar
Fan, Y, Arbab, AAI, Zhang, H, Yang, Y and Yang, Z (2021) Lactation associated genes revealed in Holstein dairy cows by weighted gene co-expression network analysis (WGCNA). Animals 106, 5018–5028.Google Scholar
Han, B, Zhang, L, Luo, B, Ni, Y, Bansal, N and Zhou, P (2021) Comparison of milk fat globule membrane and whey proteome between Dromedary and Bactrian camel. Food Chemistry 367, 130658.CrossRefGoogle ScholarPubMed
Janjanam, J, Singh, S, Jena, MK, Varshney, N, Kola, S, Kumar, S, Kaushik, JK, Grover, S, Dang, AK, Mukesh, M, Prakash, BS and Mohanty, AK (2014) Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. PLoS One 9, e102515.CrossRefGoogle ScholarPubMed
Li, C, Cai, W, Zhou, C, Yin, H, Zhang, Z, Loor, JJ, Sun, D, Zhang, Q, Liu, J and Zhang, S (2016) RNA-Seq reveals 10 novel promising candidate genes affecting milk protein concentration in the Chinese Holstein population. Reports 6, 26813.Google ScholarPubMed
Li, M, Li, Q and Zheng, Y (2020) New insights into the alterations of full spectrum amino acids in human colostrum and mature milk between different domains based on metabolomics. European Food Research and Technology 246, 1119–1128.CrossRefGoogle Scholar
Li, M, Li, Q, Song, W, Liu, Y, Zhang, X, Zheng, Y and Yue, X (2021a) Discovery of lipid biomarkers between bovine colostrum and milk using UHPLC-Q-TOF-MS lipidomics. International Dairy Journal 120, 5.CrossRefGoogle Scholar
Li, M, Yu, H, Chen, J, Abdlla, R, Liu, A, Song, W, Zhang, J, Zhang, X, Yue, X and Li, Q (2021b) Novel insights into whey protein differences between donkey and bovine milk. Food Chemistry 365, 130397.CrossRefGoogle ScholarPubMed
Li, Q, Yuan, Q, Jiang, N, Zhang, Y, Su, Z, Lv, L, Sang, X, Chen, R, Feng, Y and Chen, Q (2022a) Dihydroartemisinin regulates immune cell heterogeneity by triggering a cascade reaction of CDK and MAPK phosphorylation. Signal Transduction and Targeted Therapy 7, 222.CrossRefGoogle ScholarPubMed
Li, YZ, Wang, YY, Huang, L, Zhao, YY, Chen, LH and Zhang, C (2022b) Annexin A protein family in atherosclerosis. Clinica Chimica Acta 531, 406–417.CrossRefGoogle ScholarPubMed
Lin, Z, Song, Y, He, W and Dai, H (2016) The structure, function, and research progress of membrane associated protein A2 in diseases. Modern Applied Pharmacy in China 33, 1350–1354.Google Scholar
Liu, Y, Hou, J, Zhang, M, Seleh-Zo, E, Wang, J, Cao, B and An, X (2020) Circ-016910 sponges miR-574-5p to regulate cell physiology and milk synthesis via MAPK and PI3 K/AKT-mTOR pathways in GMECs. Journal of Cellular Physiology 235, 4198–4216.CrossRefGoogle Scholar
Malloy, CR, Sherry, AD and Jeffrey, FM (1988) Evaluation of carbon flux and substrate selection through alternate pathways involving the citric acid cycle of the heart by 13C NMR spectroscopy. Journal of Biological Chemistry 263, 6964–6971.CrossRefGoogle Scholar
Ostrowska, M, Zwierzchowski, L, Brzozowska, P, Kawecka-Grochocka, E, Żelazowska, B and Bagnicka, E (2023) The effect of single-nucleotide polymorphism in the promoter region of bovine alpha-lactalbumin (LALBA) gene on LALBA expression in milk cells and milk traits of cows. Journal of Animal Science 99, skab169.CrossRefGoogle Scholar
Rahmatalla, SA, Arends, D, Ahmed, AS, Reissmann, M and Brockmann, GA (2020) Whey protein polymorphisms in Sudanese goat breeds. Tropical Animal Health and Production 52, 1211–1222.CrossRefGoogle ScholarPubMed
Ren, R, Yang, B, Peng, H, Wang, B, Zhang, F, Hui, Y and Jia, R (2021) Analyze the protein functional properties of goat milk, cow milk and human milk based on proteomics. Dairy Science and Technology [in China] 44, 1–6.Google Scholar
Schulze, WX and Usadel, B (2010) Quantitation in mass-spectrometry-based proteomics. Annual Review of Plant Biology 61, 491–516.CrossRefGoogle ScholarPubMed
Shen, LH, Zhang, Y, Shen, Y, Su, ZT, Yu, SM, Cao, SZ and Zong, XL (2023) Effect of anemoside B4 on milk whey in clinical mastitis-affected cows elucidated using tandem mass tag (TMT)-based quantitative proteomics. Scientific Reports 12, 18829.CrossRefGoogle Scholar
Tedesco, B, Cristofani, R, Ferrari, V, Cozzi, M, Rusmini, P, Casarotto, E, Chierichetti, M, Mina, F, Galbiati, M, Piccolella, M, Crippa, V and Poletti, A (2022) Insights on human small heat shock proteins and their alterations in diseases. Frontiers in Molecular Biosciences 9, 842149.CrossRefGoogle ScholarPubMed
Wang, J and Zhao, B (2021) Analysis of the development status and trends of the world and China's milk goat industry. Chinese Journal of Animal Husbandry 57, 180–186.Google Scholar
Wang, Y, Liu, S, Tian, S, Du, R, Lin, T, Xiao, X, Wang, R, Chen, R, Geng, H, Subramanian, S, Niu, Y, Wang, Y and Yue, D (2020) C1QBP regulates apoptosis of renal cell carcinoma via modulating xanthine dehydrogenase (XDH) mediated ROS generation. International Journal of Medical Sciences 19, 842–857.CrossRefGoogle Scholar
Wasinger, VC, Cordwell, SJ, Cerpa-Poljak, A, Yan, JX and Humphery-Smith, I (1995) Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium. Electrophoresis 16, 1090–1094.CrossRefGoogle ScholarPubMed
Wu, X (2022) Research progress on functional characteristics and adulteration identification methods of goat milk. China Dairy Industry 50, 38–42 + 46.Google Scholar
Xu, H, Li, C, Mozziconacci, O, Zhu, R, Xu, Y, Tang, Y, Chen, R, Huang, Y, Holzbeierlein, JM, Schöneich, C, Huang, J and Li, B (2019) Xanthine oxidase-mediated oxidative stress promotes cancer cell-specific apoptosis. Free Radical Biology and Medicine 139, 70–79.CrossRefGoogle ScholarPubMed
Zhang, Q, Ye, Z, Yang, Q, He, X, Wang, H and Zhao, Z (2012) Upregulated expression of Annexin II is a prognostic marker for patients with gastric cancer. World Journal of Surgical Oncology 10, 103.CrossRefGoogle ScholarPubMed
Zhang, MC, Zhen, Z, Ao, J, Yuan, X and Gao, X (2018) Annexin A2 positively regulates milk synthesis and proliferation of bovine mammary epithelial cells through the mTOR signaling pathway. Journal of Cellular Physiology 233, 2464–2475.CrossRefGoogle ScholarPubMed
Zhu, J and Dingess, KA (2019) The functional power of the human milk proteome. Nutrients 11, 1834.CrossRefGoogle ScholarPubMed
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