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Sex-dependent metabolic effects of pregestational exercise on prenatally stressed mice

Published online by Cambridge University Press:  14 May 2020

Carolina Luft ,
Isadora Perez Levices ,
Leonardo Pedrazza ,
Jarbas Rodrigues de Oliveira  and
Márcio Vinícius Fagundes Donadio Open the ORCID record for Márcio Vinícius Fagundes Donadio [Opens in a new window]
Carolina Luft
Affiliation:
Laboratory of Pediatric Physical Activity, Infant Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil Laboratory of Cellular Biophysics and Inflammation, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do SulCEP 90619-900, Brazil
Isadora Perez Levices
Affiliation:
Laboratory of Pediatric Physical Activity, Infant Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil
Leonardo Pedrazza
Affiliation:
Laboratory of Ubiquitination and Cellular Signalization, IDIBELL, Campus de Bellvitge, Universitat de Barcelona, L’Hospitalet de Llobregat, ES-08907Barcelona, Spain
Jarbas Rodrigues de Oliveira
Affiliation:
Laboratory of Cellular Biophysics and Inflammation, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do SulCEP 90619-900, Brazil
Márcio Vinícius Fagundes Donadio*
Affiliation:
Laboratory of Pediatric Physical Activity, Infant Center, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil Laboratory of Cellular Biophysics and Inflammation, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do SulCEP 90619-900, Brazil
*
Address for correspondence: Professor Márcio V. F. Donadio, Centro Infant, Pontifícia Universidade Católica do Rio Grande do Sul – Av. Ipiranga, 6690, 2º andar, Porto Alegre, Rio Grande do Sul CEP 90610-000, Brazil. Email: mdonadio@pucrs.br
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Abstract

Stressful events during the prenatal period have been related to hyperactive hypothalamic–pituitary–adrenal (HPA) axis responses as well as metabolic changes in adult life. Moreover, regular exercise may contribute to the improvement of the symptoms associated with stress and stress-related chronic diseases. Therefore, this study aims to investigate the effects of exercise, before the gestation period, on the metabolic changes induced by prenatal stress in adult mice. Female Balb/c mice were divided into three groups: control (CON), prenatal restraint stress (PNS) and exercise before the gestational period plus PNS (EX + PNS). When adults, the plasmatic biochemical analysis, oxidative stress, gene expression of metabolic-related receptors and sex differences were assessed in the offspring. Prenatal stress decreased neonatal and adult body weight when compared to the pregestational exercise group. Moreover, prenatal stress was associated with reduced body weight in adult males. PNS and EX + PNS females showed decreased hepatic catalase. Pregestational exercise prevented the stress-induced cholesterol increase in females but did not prevent the liver mRNA expression reduction on the peroxisome proliferator-activated receptors (PPARs) α and γ in PNS females. Conversely, PNS and EX + PNS males showed an increased PPARα mRNA expression. In conclusion, pregestational exercise prevented some effects of prenatal stress on metabolic markers in a sex-specific manner.

Keywords

Early life stresslivertreadmillsex-difference
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 12 , Issue 2 , April 2021 , pp. 271 - 279
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2020

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