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The elucidation of the life cycle of Saccocoelioides nanii Szidat, 1954 (Digenea: Haploporidae) using molecular techniques

Published online by Cambridge University Press:  03 November 2023

L.E. Martinez ,
C. Gilardoni Open the ORCID record for C. Gilardoni [Opens in a new window] ,
C. Medina ,
F. Cremonte  and
J.A. Etchegoin
L.E. Martinez
Affiliation:
Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), CONICET-Universidad Nacional de Mar del Plata, Centro de Asociación Simple CIC-PBA, Juan B. Justo 2550 (7600), Mar del Plata, Buenos Aires, Argentina
C. Gilardoni*
Affiliation:
Instituto de Biología de Organismos Marinos (IBIOMAR-CONICET), Boulevard Brown 2915, (9120), Puerto Madryn, Chubut, Argentina
C. Medina
Affiliation:
Instituto de Diversidad y Evolución Austral (IDEAus-CONICET), Boulevard Brown 2915, (9120), Puerto Madryn, Chubut, Argentina
F. Cremonte
Affiliation:
Instituto de Biología de Organismos Marinos (IBIOMAR-CONICET), Boulevard Brown 2915, (9120), Puerto Madryn, Chubut, Argentina
J.A. Etchegoin
Affiliation:
Instituto de Investigaciones en Producción Sanidad y Ambiente (IIPROSAM), CONICET-Universidad Nacional de Mar del Plata, Centro de Asociación Simple CIC-PBA, Juan B. Justo 2550 (7600), Mar del Plata, Buenos Aires, Argentina
*
Corresponding author: C. Gilardoni; Email: gilardonicarmen@gmail.com
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Abstract

In South America, the knowledge of trematode diversity parasitizing freshwater fishes is still scarce, as less than 5% of the freshwater fish fauna has been examined for parasites. A similar situation applies to studies on digenean life cycles, which have become increasingly rare. Among the digenean families parasitizing freshwater fishes in the region, Haploporidae is considered the richest in species diversity. However, information about the developmental stages of haploporid life cycles remains fragmentary. Particularly, in Argentina, nine cercariae attributed to the family Haploporidae have been described using morphological analysis, and only two life cycles of this family have been completely elucidated. In this study a new type of cercaria, morphologically assigned to the family Haploporidae and collected from the snail Heleobia parchappii (Cochliopidae) in Los Padres shallow lake, Buenos Aires province, was identified using morphological and molecular techniques. The molecular analysis, based on 28S and ITS2 sequences, revealed that the cercariae were 100% identical to adult specimens of Saccocoelioides nanii (Haploporidae) parasitizing the fish Prochilodus lineatus (Prochilodontidae) from Los Talas, Buenos Aires province. Our results not only provide information about the life cycle of S. nanii but also show that a molecular and morphological approach can be extremely useful in identifying the developmental stages of digeneans and elucidating their life cycles.

Keywords

SaccocoelioidesHaploporidaeLife cyclegeneticArgentina
Type
Research Paper
Information
Journal of Helminthology , Volume 97 , 2023 , e80
Copyright
© The Author(s), 2023. Published by Cambridge University Press

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