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Monorchis lewisi n. sp. (Trematoda: Monorchiidae) from the surf bream, Acanthopagrus australis (Sparidae), in Moreton Bay, Australia

Published online by Cambridge University Press:  17 November 2017

T.H. Cribb*
The University of Queensland, School of Biological Sciences, St Lucia, Queensland, 4072, Australia
N.Q.-X. Wee
The University of Queensland, School of Biological Sciences, St Lucia, Queensland, 4072, Australia
R.A. Bray
Department of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, United Kingdom
S.C. Cutmore
The University of Queensland, School of Biological Sciences, St Lucia, Queensland, 4072, Australia


We describe Monorchis lewisi n. sp. (Monorchiidae) from the surf bream, Acanthopagrus australis (Günther, 1859) (Sparidae), in Moreton Bay, eastern Australia. The new species differs from most existing species of Monorchis Monticelli, 1893 in its possession of an elongate I-shaped excretory vesicle, and from other congeners in the relative configuration of the gut and suckers. Ovipusillus mayu Dove & Cribb, 1998 is re-reported from Gnathanodon speciosus (Forsskål, 1775) (Carangidae) from Moreton Bay. We report new second internal transcribed spacer (ITS2) and 28S rDNA sequence data for both species. Bayesian inference and Maximum Likelihood analyses of the 28S rDNA dataset suggest that existing subfamily and genus concepts within the family require substantial revision.

Research Papers
Copyright © Cambridge University Press 2017 

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This article was registered in the Official Register of Zoological Nomenclature (ZooBank) as 672B5E9E-5AAA-48B2-91C6-35141BCA46AF. This article was published as an Online First article on the online publication date shown on this page. The article should be cited by using the doi number. This is the Version of Record.


Ankenbrand, M.J., Keller, A., Wolf, M., Schultz, J. & Forster, F. (2015) ITS2 Database V: twice as much. Molecular Biology and Evolution 32, 30303032.CrossRefGoogle ScholarPubMed
Atopkin, D.M., Besprozvannykh, V.V., Ngo, H.D., Van Ha, N., Van Tang, N., Ermolenko, A.V. & Beloded, A.Y. (2017) Morphometric and molecular data of the two digenean species Lasiotocus lizae Liu, 2002 (Monorchiidae) and Paucivitellosus vietnamensis sp. n. (Bivesiculidae) from mullet fish in Tonkin Bay, Vietnam. Journal of Helminthology 91, 346355.CrossRefGoogle Scholar
Besprozvannykh, V.V., Ermolenko, A.V. & Atopkin, D.M. (2012) The life cycle of Asymphylodora perccotti sp. n. (Trematoda: Lissorchiidae) in the Russian Southern Far East. Parasitology International 61, 235241.CrossRefGoogle Scholar
Bray, R.A. & Cribb, T.H. (2012) Reorganisation of the superfamily Lepocreadioidea Odhner, 1905 based on an inferred molecular phylogeny. Systematic Parasitology 83, 169177.CrossRefGoogle ScholarPubMed
Bray, R.A. & Gaevskaya, A.V. (1993) Bathymonorchis polyipni (Reimer, 1985) n. g., n. comb. (Digenea: Monorchiidae) from bathypelagic fishes of the eastern mid-Atlantic Ocean. Systematic Parasitology 26, 9195.CrossRefGoogle Scholar
Bray, R.A., Justine, J-L & Cribb, T.H. (2009a) Cableia balistidicola n. sp (Digenea, Monorchiidae) from Pacific Ocean balistids (Tetraodontiformes) and new reports of Cableia pudica Bray, Cribb and Barker, 1996 in temperate Australian monacanthids. Parasitology International 58, 341345.CrossRefGoogle Scholar
Bray, R.A., Waeschenbach, A., Cribb, T.H., Weedall, G.D., Dyal, P. & Littlewood, D.T.J. (2009b) The phylogeny of the Lepocreadioidea (Platyhelminthes, Digenea) inferred from nuclear and mitochondrial genes: implications for their systematics and evolution. Acta Parasitologica 54, 310329.CrossRefGoogle Scholar
Bray, R.A., Palm, H.W., Cutmore, S.C. & Cribb, T.H. (2017) Three members of Opisthomonorcheides Parukhin, 1966 (Digenea: Monorchiidae) from carangid fishes (Perciformes) from Indonesia, with a review of the genus. Systematic Parasitology 94, 443462.CrossRefGoogle ScholarPubMed
Cribb, T.H. & Bray, R.A. (2010) Gut wash, body soak, blender and heat-fixation: approaches to the effective collection, fixation and preservation of trematodes of fishes. Systematic Parasitology 76, 17.CrossRefGoogle ScholarPubMed
Cribb, T.H. & Gibson, D.I. (2010) Monorchiidae Odhner, 1911. Available at World Register of Marine Species at (accessed 29 March 2017).Google Scholar
Cribb, T.H., Anderson, G.R., Adlard, R.D. & Bray, R.A. (1998) A DNA-based demonstration of a three-host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). International Journal for Parasitology 28, 17911795.CrossRefGoogle Scholar
Cribb, T.H., Bray, R.A., Littlewood, D.T.J., Pichelin, S. & Herniou, E.A. (2001) The Digenea. pp. 168185 in Littlewood, D.T.J. & Bray, R.A. (Eds) Interrelationships of the platyhelminthes. London, Taylor & Francis.Google Scholar
Cribb, T.H., Bray, R.A., Diaz, P.E., Huston, D.C., Kudlai, O., Martin, S.B., Yong, RQ-Y & Cutmore, S.C. (2016) Trematodes of fishes of the Indo-west Pacific: told and untold richness. Systematic Parasitology 93, 237247.CrossRefGoogle ScholarPubMed
Cutmore, S.C., Miller, T.L., Curran, S.S., Bennett, M.B. & Cribb, T.H. (2013) Phylogenetic relationships of the Gorgoderidae (Platyhelminthes: Trematoda), including the proposal of a new subfamily (Degeneriinae n. subfam.). Parasitology Research 112, 30633074.CrossRefGoogle Scholar
Cutmore, S.C., Diggles, B.K. & Cribb, T.H. (2016) Transversotrema Witenberg, 1944 (Trematoda: Transversotrematidae) from inshore fishes of Australia: description of a new species and significant range extensions for three congeners. Systematic Parasitology 93, 639652.CrossRefGoogle ScholarPubMed
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772.CrossRefGoogle ScholarPubMed
Dove, A.D.M. & Cribb, T.H. (1998) Two new genera, Provitellus and Ovipusillus, and four new species of Monorchiidae (Digenea) from carangid fishes of Queensland, Australia. Systematic Parasitology 40, 2133.CrossRefGoogle Scholar
Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 17921797.CrossRefGoogle ScholarPubMed
Gibson, D. (2013) Monorchis Monticelli, 1893.Available at World Register of Marine Species at (accessed 23 March 2017).Google Scholar
Johnson, J.W. (2010) Fishes of the Moreton Bay Marine Park and adjacent continental shelf waters, Queensland, Australia. Memoirs of the Queensland Museum 54, 299353.Google Scholar
Jousson, O. & Bartoli, P. (2002) Species diversity among the genus Monorchis (Digenea: Monorchiidae) parasitic in marine teleosts: molecular, morphological and morphometrical studies with a description of Monorchis blennii n. sp. Parasitology Research 88, 230241.Google ScholarPubMed
Keller, A., Schleicher, T., Schultz, J., Muller, T., Dandekar, T. & Wolf, M. (2009) 5.8S–28S rRNA interaction and HMM-based ITS2 annotation. Gene 430, 5057.CrossRefGoogle ScholarPubMed
Littlewood, D.T.J. (1994) Molecular phylogenetics of cupped oysters based on partial 28S ribosomal RNA gene sequences. Molecular Phylogenetics and Evolution 3, 221229.CrossRefGoogle Scholar
Littlewood, D.T.J., Rohde, K. & Clough, K.A. (1997) Parasite speciation within or between host species? – phylogenetic evidence from site-specific polystome monogeneans. International Journal for Parasitology 27, 12891297.CrossRefGoogle ScholarPubMed
Littlewood, D.T.J., Curini-Galletti, M. & Herniou, E.A. (2000) The interrelationships of Proseriata (Platyhelminthes: Seriata) tested with molecules and morphology. Molecular Phylogenetics and Evolution 16, 449466.CrossRefGoogle ScholarPubMed
Maddison, W.P. & Maddison, D.R. (2017) Mesquite: A modular system for evolutionary analysis, Version 3.2. 2017. Available at (accessed 31 May 2017).Google Scholar
Madhavi, R. (1977) Some new digenetic trematodes (Monorchiidae) from marine fishes of Waltair Coast, Bay of Bengal. pp. 233246 in Villa-Ramírez, B. (Ed.) Excerta parasitologica en memoria del Doctor Eduardo Caballero y Caballero. Mexico City, Universidad Nacional Autónoma de México.Google Scholar
Madhavi, R. (2008) Family Monorchiidae Odhner, 1911. pp. 145175 in Bray, R.A., Gibson, D.I. & Jones, A. (Eds) Keys to the Trematoda, Volume 3. Wallingford, CAB International and Natural History Museum.CrossRefGoogle Scholar
Mamaev, Y.L. (1971) [Helminths of some commercial fishes in the Gulf of Tonkin.] pp. 127190 in Oshmarin, P.G., Mamaev, Y.L. & Lebedev, B.I. (Eds) [Helminths of animals of south-east Asia.] Moscow, Izdatel'stvo Nauka (in Russian).Google Scholar
McNamara, M.K.A. & Cribb, T.H. (2011) Taxonomy, host specificity and dietary implications of Hurleytrematoides (Digenea: Monorchiidae) from chaetodontid fishes on the Great Barrier Reef. Parasitology International 60, 255269.CrossRefGoogle ScholarPubMed
McNamara, M.K.A., Miller, T.L. & Cribb, T.H. (2014) Evidence for extensive cryptic speciation in trematodes of butterflyfishes (Chaetodontidae) of the tropical Indo-West Pacific. International Journal for Parasitology 44, 3748.CrossRefGoogle ScholarPubMed
Miller, M.A., Pfeiler, E. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, Louisiana, 14 November, pp. 18.Google Scholar
Miller, T.L., Bray, R.A. & Cribb, T.H. (2011) Taxonomic approaches to and interpretation of host-specificity of trematodes of fishes: lessons from the Great Barrier Reef. Parasitology 138, 17101722.CrossRefGoogle ScholarPubMed
Morgan, J.A.T. & Blair, D. (1995) Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group. Parasitology 111, 609615.CrossRefGoogle ScholarPubMed
Nahhas, F.M. & Cable, R.M. (1964) Digenetic and aspidogastrid trematodes from marine fishes of Curaçao and Jamaica. Tulane Studies in Zoology 11, 169228.CrossRefGoogle Scholar
Nahhas, F.M. & Powell, E.C. (1965) Monorchiidae (Trematoda) from fishes of Apalachee Bay, Gulf of Mexico. Journal of Parasitology 51, 1620.CrossRefGoogle ScholarPubMed
Olson, P.D., Cribb, T.H., Tkach, V.V., Bray, R.A. & Littlewood, D.T.J. (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33, 733755.CrossRefGoogle Scholar
Overstreet, R.M. (1969) Digenetic trematodes of marine teleost fishes from Biscayne Bay, Florida. Tulane Studies in Zoology and Botany 15, 119176.Google Scholar
Reiczigel, J., Abonyi-Toth, Z. & Singer, J. (2008) An exact confidence set for two binomial proportions and exact unconditional confidence intervals for the difference and ratio of proportions. Computational Statistics & Data Analysis 52, 50465053.CrossRefGoogle Scholar
Reimer, L.W. (1985) Zwei neuer Arten der Monorchiidae (Digenea) aus Fischen der Straβe von Moçambique. Angewandte Parasitologie 26, 225228.Google Scholar
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539542.CrossRefGoogle ScholarPubMed
Rozsa, L., Reiczigel, J. & Majoros, G. (2000) Quantifying parasites in samples of hosts. Journal of Parasitology 86, 228232.CrossRefGoogle ScholarPubMed
Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Press.Google Scholar
Searle, E.L., Cutmore, S.C. & Cribb, T.H. (2014) Monorchiid trematodes of the painted sweetlips, Diagramma labiosum (Perciformes, Haemulidae), from the southern Great Barrier Reef, including a new genus and three new species. Systematic Parasitology 88, 195211.CrossRefGoogle Scholar
Snyder, S.D. & Tkach, V.V. (2001) Phylogenetic and biogeographical relationships among some Holarctic frog lung flukes (Digenea: Haematoloechidae). Journal of Parasitology 87, 14331440.CrossRefGoogle Scholar
Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology 57, 758771.CrossRefGoogle ScholarPubMed
Tkach, V.V., Pawlowski, J., Mariaux, J. & Swiderski, Z. (2001) Molecular phylogeny of the suborder Plagiorchiata and its position in the system of Digenea. pp. 186193 in Littlewood, D.T.J. & Bray, R.A. (Eds) Interrelationships of the Platyhelminthes. London, Taylor & Francis.Google Scholar
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Monorchis lewisi n. sp. (Trematoda: Monorchiidae) from the surf bream, Acanthopagrus australis (Sparidae), in Moreton Bay, Australia
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