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Adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi in jirds

Published online by Cambridge University Press:  12 April 2024

Y. Srivastava ,
S. Rathaur ,
Y.P. Bhandari ,
M.V.R. Reddy  and
B.C. Harinath
Y. Srivastava
Affiliation:
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221 005, India
S. Rathaur*
Affiliation:
Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221 005, India
Y.P. Bhandari
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India
M.V.R. Reddy
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India
B.C. Harinath
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India
*
*Author for correspondence E-mail: sushma@banaras.ernet.in
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Abstract

A 175 kDa antigen fraction with collagenase activity was isolated and purified from somatic extracts of adult Setaria cervi females using column chromatography involving consecutive steps of DEAE-Sepharose CL6B and Sephadex G-100. The optimum pH for 175 kDa collagenase was found to be pH 7.0. Sensitivities to a variety of inhibitors and activators indicated that the 175 kDa coIlagenolytic enzyme was metalloserine in nature. The enzyme hydrolysed a variety of protein substrates such as haemoglobin, casein, azocasein (general substrates) and collagen, FALGPA (furanoyl-acryloyl-leu-gly-pro-ala), the specific substrate of collagenase. The enzyme showed 57% inhibition by jird anti-somatic collagenase antibodies and reacted insignificantly with normal jird sera. Further analysis was undertaken on the immunoprophylactic potential of 175 kDa collagenase in inducing immunity against Brugia malayi (a human filarial parasite) in jirds (Meriones unguiculatus) in vitro and in situ. Immune sera of jirds raised against this antigen promoted partial adherence of peritoneal exudate cells to B. malayi microfilariae (mf) and infective larvae (L3) in vitro and induced partial cytotoxicity to the parasites within 48 h. The anti-S. cervi 175 kDa antigen serum was more effective in inducing cytotoxicity to B. malayi L3, than mf. In the microchambers implanted inside immune jirds, host cells could migrate and adhere to the mf and infective larvae thereby killing them partially within 48 h.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 4 , December 2004 , pp. 347 - 352
Copyright
Copyright © Cambridge University Press 2004

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