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An in vivo appraisal of Punica granatum peel extract’s ultrastructural effect on cystic echinococcosis in mice

Published online by Cambridge University Press:  13 May 2024

Nashaat E. Nassef ,
Sawsan S. Shendi ,
Abdel-Gawad E. Saad ,
Nancy M. Harba ,
Engy V.N. Beshay ,
Asmaa Shams El-Dein Mohamed  and
Marwa A. Gouda Open the ORCID record for Marwa A. Gouda [Opens in a new window]
Nashaat E. Nassef
Affiliation:
Parasitology Department, Faculty of Medicine, Menoufia University, Yassin Abdel Ghaffar St. from Gamal Abdel Nasser St., Shebin El-Kom, Menoufia, Egypt
Sawsan S. Shendi
Affiliation:
Department of Clinical and Molecular Parasitology Department, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt
Abdel-Gawad E. Saad
Affiliation:
Department of Clinical and Molecular Parasitology Department, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt
Nancy M. Harba
Affiliation:
Parasitology Department, Faculty of Medicine, Menoufia University, Yassin Abdel Ghaffar St. from Gamal Abdel Nasser St., Shebin El-Kom, Menoufia, Egypt
Engy V.N. Beshay
Affiliation:
Parasitology Department, Faculty of Medicine, Menoufia University, Yassin Abdel Ghaffar St. from Gamal Abdel Nasser St., Shebin El-Kom, Menoufia, Egypt
Asmaa Shams El-Dein Mohamed
Affiliation:
Pathology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Egypt
Marwa A. Gouda*
Affiliation:
Department of Clinical and Molecular Parasitology Department, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt
*
Corresponding author: Marwa A. Gouda; Email: marwa_goda@liver.menofia.edu.eg
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Abstract

In the past decade, interest has significantly increased regarding the medicinal and nutritional benefits of pomegranate (Punica granatum) peel. This study examined the effects of using pomegranate peel extract (PGE) alone and in combination with albendazole (ABZ) on ultrastructural and immunological changes in cystic echinococcosis in laboratory-infected mice. Results revealed that the smallest hydatid cyst size and weight (0.48 ± 0.47mm, 0.17 ± 0.18 gm) with the highest drug efficacy (56.2%) was detected in the PGE + ABZ group, which also exhibited marked histopathological improvement. Ultrastructural changes recorded by transmission electron microscopy including fragmentation of the nucleus, glycogen depletion, and multiple lysosomes in vacuolated cytoplasm were more often observed in PGE + ABZ group. IFN-γ levels were significantly increased in the group treated with ABZ, with a notable reduction following PGE treatment, whether administered alone or in combination with ABZ. Thus, PGE enhanced the therapeutic efficiency of ABZ, with improvement in histopathological and ultrastructural changes.

Keywords

EchinococcosisPunica granumTEMalbendazoleIFN-γ
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e40
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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