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Biosynthetic labelling of the excretory and secretory antigens of Toxocara canis larvae

Published online by Cambridge University Press:  18 November 2009

K. Sugane ,
M. J. Howell  and
W. L. Nicholas
K. Sugane
Affiliation:
Department of Parasitology, School of Medicine, Yokohama City University, Urafime-cho 2-33, Minami-ku, Yokohama City, Japan, and Department of Zoology, Faculty of Science, Australian National University, GPO Box 4, Canberra ACT 2601Australia
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Abstract

Toxocara canis larvae were cultured in vitro in medium containing [35S-]methionine for six days. The medium and the larval tissues were analysed for biosynthetically labelled polypcptides by sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography. Immunoprecipitates with positive and negative human antiserum were similarly analysed, using Staphylococcus aureus to absorb immunocom plexes. The larvae secrete biosynthctically labelled polypeptides into the medium, with three major polypcptides of molecular weights between 99 and 110 x 103 the major constituents. Both of these react strongly with human IgG in human positive scra. Many polypeptidcs become labelled in the larval tissue, but only one polypeptidc with similar molecular weight to the ES antigens, strongly reacted with human IgG.

Type
Research Article
Information
Journal of Helminthology , Volume 59 , Issue 2 , June 1985 , pp. 147 - 151
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

De Savigny, D. H. (1975) In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. Journal of Parasitology, 61, 781–782.Google Scholar
De Savigny, D. H., Voller, A. & Woodruff, A. W. (1979) Toxocariasis: serological diagnosis by enzyme immunoassay. Journal of Clinical Pathology, 32, 284–288.CrossRefGoogle Scholar
Fairbairn, D. (1961) The in vitro hatching of Ascaris lumbricoides. Canadian Journal of Zoology, 39, 153–167.CrossRefGoogle Scholar
Irving, D.O. & Howell, M.J. (1981) Preparation and in vitro translation of mRNA from Fasciola hepatica. Molecular and Biochemical Parasitology, 4, 337–348.CrossRefGoogle Scholar
Irving, D. O. & Howell, M. J. (1982) Characterization of excretory-secretory antigens of Fasciola hepatica. Parasitology, 85, 179–188.CrossRefGoogle Scholar
Josephs, D. S., Bhinder, P. & Thompson, A. R. (1981) The prevalence of Toxocara infection in a child population. Public Health, UK, 95, 273–275.Google Scholar
Sugane, K. & Oshima, T. (1983) Purification and characterization of excretory and secretory antigen of Toxocara canis larvae. Immunology, 50, 113–120.Google Scholar
Van Knapen, F., Van Leusden, J., Polderman, A. M. & Franchimont, J. H. (1983) Visceral larva migrans: examinations by means of enzyme-linked immunosorbcnt assay of human scra for antibodies to excretory and secretory antigens of the second stage larvae of Toxocara canis. Zeitschrift für Parasitenkunde, 69, 113–118.CrossRefGoogle Scholar
Welch, J. S., Symons, M. H. & Dobson, C. (1983) Immunodiagnosis of parasitic zoonoses; purification of Toxocara canis antigens by affinity chromatography. International Journal for Parasitology, 13, 171–178.CrossRefGoogle Scholar