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A competitive enzyme-linked immunosorbent assay for rapid detection of antibodies against Trichinella spiralis and T. britovi – one test for humans and swine

Published online by Cambridge University Press:  23 November 2017

M. Gnjatovic ,
A. Gruden-Movsesijan ,
N. Miladinovic-Tasic ,
N. Ilic ,
S. Vasilev ,
J. Cvetkovic  and
Lj. Sofronic-Milosavljevic
M. Gnjatovic*
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
A. Gruden-Movsesijan
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
N. Miladinovic-Tasic
Affiliation:
Public Health Institute Niš, Blvd. Dr Zorana Djindjica 50, 18000 Nis, Serbia
N. Ilic
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
S. Vasilev
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
J. Cvetkovic
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
Lj. Sofronic-Milosavljevic
Affiliation:
Serbian National Reference Laboratory for Trichinellosis (NRLT), Institute for the Application of Nuclear Energy – INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia
*
Author for correspondence: M. Gnjatovic, Fax: +381 2618 724, E-mail: marijad@inep.co.rs
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Abstract

Infection with parasites from the Trichinella genus occurs in many vertebrates but disease only occurs in humans (trichinellosis). Humans are infected after the consumption of raw or undercooked meat from infected wild or domestic animals (usually swine or horses). Using the monoclonal antibody (mAb) 7C2C5, specific for an epitope unique to the muscle larvae of the genus Trichinella, we have developed a competitive enzyme-linked immunosorbent assay (c-ELISA) that enables the rapid detection of Trichinella-specific antibodies in sera originating from two different host species (human, swine) infected with either Trichinella spiralis or Trichinella britovi. This novel c-ELISA exhibited 100% specificity and sensitivity, as confirmed by a Western blot test. The assay is easy to use (one incubation step), and the time required for the procedure (45 min) is shorter than in any other ELISA format. This test could be useful for both the detection and surveillance of Trichinella infections.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 1 , January 2019 , pp. 33 - 41
Copyright
Copyright © Cambridge University Press 2017 

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