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Counter current immunoelectrophoresis, a new technique for the rapid serodiagnosis of porcine cysticercosis

Published online by Cambridge University Press:  05 June 2009

K. M. L. Pathak ,
S. N. S. Gaur  and
S. K. Garg
K. M. L. Pathak
Affiliation:
Collage of Veterinary Sciences, G. B. Pant University of Aariculture & Technology, Paninagar, India
S. N. S. Gaur
Affiliation:
Collage of Veterinary Sciences, G. B. Pant University of Aariculture & Technology, Paninagar, India
S. K. Garg
Affiliation:
Collage of Veterinary Sciences, G. B. Pant University of Aariculture & Technology, Paninagar, India
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Abstract

Counter current Immunoelectrophoresis was used to detect porcine cysticcrcosis and water soluble extracts of scolex and of scolex with cyst wall were used as antigens. Serum samples from 40 pigs infected with Cysticercus cellulosae, five infected with C. tenuicollis and five with hydatid cysts, and 15 healthy pigs were tested. A sharp and thick concave precipitin band was observed at the point of interaction of antigen and antibody within 90min in 39 sera from infected pigs (97–5%). The precipitin reaction was better in barbitone buffer of pH 8–6 with the well distance at 6 mm. No false or cross reaction were found and the test was rapid and sensitive.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 4 , December 1984 , pp. 321 - 324
Copyright
Copyright © Cambridge University Press 1984

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References

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