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Cryopreservation of Dirofilaria immitis microfilariae and third-stage larvae

Published online by Cambridge University Press:  05 June 2009

J. B. Lok ,
M. Mika-Grieve  and
R. B. Grieve
J. B. Lok
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, U.S.A.
M. Mika-Grieve
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, U.S.A.
R. B. Grieve
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, U.S.A.
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Abstract

Microfilariae of Dirofilaria immitis retained their infectivity for susceptible mosquitoes after cooling to −196°C in the presence of 5% dimethylsulphoxide (Me2SO) using a two-step cooling sequence. Motility and in vitro development of cryopreserved microfilariae also compared favourably with unfrozen controls. Third-stage larvae frozen by the same cooling sequence in the presence of either 5% Me2SO or 16% hydroxyethyl starch were motile upon thawing. Thawed larvae completed the third- to fourth-stage moult in vitro at a frequency approximately 5 to 10% of that seen in unfrozen controls.

Type
Research Note
Information
Journal of Helminthology , Volume 57 , Issue 4 , December 1983 , pp. 319 - 324
Copyright
Copyright © Cambridge University Press 1983

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References

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