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Detection of serum cytokines before and after pharmacological and surgical treatment in patients with cystic echinococcosis

Published online by Cambridge University Press:  02 March 2015

M.I. Naik ,
R.K. Tenguria  and
E. Haq
M.I. Naik*
Affiliation:
Department of Microbiology, Govt. Motilal Vigyan MahavidyalyaBhopal462 008MP, India
R.K. Tenguria
Affiliation:
Department of Microbiology, Govt. Motilal Vigyan MahavidyalyaBhopal462 008MP, India
E. Haq
Affiliation:
Department of Biotechnology, University of Kashmir, Hazratbal Srinagar-Jammu and Kashmir-190 006, India
*
* E-mail: irfan_967@yahoo.co.in
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Abstract

Human cystic echinococcosis (CE), caused by Echinococcus granulosus, is one of the most important and widespread parasitic zoonoses. One of the problems that can be encountered after treating CE patients is the risk of post-surgical relapses or treatment failure, thus a long-term clinical and serological follow-up is required to evaluate the success or failure of therapy. In the present study immunological markers have been identified to indicate the effectiveness of pharmacological and surgical treatments. The relationship between serum cytokine levels and the outcome of chemotherapy and surgery was evaluated in 50 patients with CE. Serum interleukin (IL)-4, IL-10 and interferon-gamma (IFN-γ) concentrations were determined by enzyme-linked immunosorbent assay (ELISA) before and after pharmacological and surgical treatment. Serum cytokine levels of IL-4, IL-10 and IFN-γ were elevated in a significant proportion of patients during the active stage of disease. IL-4, IL-10 and IFN-γ were measurable in 41 (82%), 37 (74%) and 25 (50%) patients before the treatment. Clinical and radiological assessment of patients 2 years after pharmacological treatment has shown that 48 of 50 patients responded to treatment. IL-4 and IL-10 levels were decreased significantly (P< 0.05) in these patients. Conversely, patients who did not respond showed high levels of IL-4 and IL-10 and undetectable levels of IFN-γ. Hence these results suggest that serum IL-4 and IL-10 detection may be useful in the follow-up of patients with CE.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 91 - 95
Copyright
Copyright © Cambridge University Press 2015 

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