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    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Ostoa-Saloma, P. Morales-Montor, J. and Larralde, C. 2011. Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge. Journal of Biomedicine and Biotechnology, Vol. 2011, p. 1.

    Zhu, Jinbo Li, Tao Hu, Jiming and Wang, Erkang 2010. A novel dot-blot DNAzyme-linked aptamer assay for protein detection. Analytical and Bioanalytical Chemistry, Vol. 397, Issue. 7, p. 2923.


Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

  • E. Hernández-Cruz (a1), J.J. González-Cabriales (a1), C. Ordaz-Pichardo (a1), N.I. de la Cruz-Hernández (a2) and G.H. Flores-Gutiérrez (a1)
  • DOI:
  • Published online: 25 March 2009

An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.

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