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Fasciola hepatica: development of monoclonal antibodies against somatic antigens and their characterization by ultrastructural localization of antibody binding

Published online by Cambridge University Press:  05 June 2009

R. E. B. Hanna ,
A. G. Trudgett  and
A. Anderson
R. E. B. Hanna
Affiliation:
Department of Zoology and Department of Microbiology and Immunobiology, Queen's University, Belfast, Northern Ireland
A. G. Trudgett
Affiliation:
Department of Zoology and Department of Microbiology and Immunobiology, Queen's University, Belfast, Northern Ireland
A. Anderson
Affiliation:
Department of Zoology and Department of Microbiology and Immunobiology, Queen's University, Belfast, Northern Ireland
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Abstract

A series of monoclonal antibodies was prepared against tegumental and internal antigens of Fasciola hepatica by immunizing mice with whole adult-fluke homogenates prior to harvesting the splenic lymphocytes for fusion. Preliminary screening by the Indirect Fluorescent Antibody technique indicated the occurrence of discrete groups of monoclonals differing from one another in tissue-specificity but within which IFA labelling patterns were fairly consistent. Representative hybridomas for 5 of these groups were stabilized and used to produce ascites fluid in mice. By application of an immunogold labelling technique it was possible to map the distribution of antigens for which each monoclonal antibody had affinity throughout the tissues of 4-week and 12-week flukes. Several monoclonals specifically labelled antigenic determinants on the important tegumental antigen T1. However the distribution of gold colloid labelling suggested that epitopes other than that normally exposed to the infected host were recognized; and several monoclonals specifically attached to T1 antigen in the tegument of juvenile worms only. The glycocalyx of the gut and excretory system of flukes shared T1 antigenicity with the tegument. Monoclonal antibodies were produced against an internal immunogen associated with ribosomes and heterochromatin in active protein-producing cells, and against interstitial material of adult flukes. Monoclonals against antigens in parenchymal cell cytoplasm and in mature vitelline cells were recognized but the corresponding hybridomas were not stabilized.

Keywords

Fasciola hepaticamonoclonal antibodiesimmunogold labelling
Type
Research Papers
Information
Journal of Helminthology , Volume 62 , Issue 1 , March 1988 , pp. 15 - 28
Copyright
Copyright © Cambridge University Press 1988

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References

REFERENCES

Beesley, J. E. (1985) Colloidal gold probes for parasite antigens. Parasitology Today, 1, 145–146.CrossRefGoogle ScholarPubMed
Dermott, S. S. E. (1967) Some ultrastructural studies on the common liver fluke, Fasciola hepatica. PhD thesis, Queen's University, Belfast.Google Scholar
Gallagher, S. S. E. & Threadgold, L. T. (1967) Electron microscope studies of Fasciola hepatica. II. The interrelationship of the parenchyma with other organ systems. Parasitology, 57, 627–632.CrossRefGoogle Scholar
Hanna, R. E. B. (1976) Fasciola hepatica: a light and electron autoradiographic study of shell-protein and glycogen synthesis by vitelline follicles in tissue slices. Experimental Parasitology, 39, 18–28.CrossRefGoogle ScholarPubMed
Hanna, R. E. B. (1980a) Fasciola hepatica: an immunofluorescent study of antigenic changes in the tegument during development in the rat and the sheep. Experimental Parasitology, 50, 155–170.CrossRefGoogle ScholarPubMed
Hanna, R. E. B. (1980b) Fasciola hepatica: glycocalyx replacement in the juvenile as a possible mechanism for protection against host immunity. Experimental Parasitology, 50, 103–114.CrossRefGoogle ScholarPubMed
Hanna, R. E. B. (1980c) Fasciola hepatica: autoradiography of protein synthesis, transport and secretion by the tegument. Experimental Parasitology, 50, 297–304.CrossRefGoogle ScholarPubMed
Hanna, R. E. B., Anderson, A. & Trudgett., A. G. (1988) Fasciola hepatica: studies on vaccination of rats and mice with a surface antigen prepared from fluke homogenate by means of a monoclonal antibody. Research in Veterinary Science, (in press).CrossRefGoogle ScholarPubMed
Hanna, R. E. B. & Hillyer, G. V. (1984) Fasciola hepatica and Schistosoma mansoni: immunofluorescent antigen localization and cross-reactivity. Experimental Parasitology, 57, 1–14.CrossRefGoogle ScholarPubMed
Hanna, R. E. B. & Trudgett, A. G. (1983) Fasciola hepatica: development of monoclonal antibodies and their use to characterize a glycocalyx antigen in migrating flukes. Parasite Immunology, 5, 409–425.CrossRefGoogle ScholarPubMed
Hillyer, G. V. & Sagramoso De Ateca, L. (1979) Immunity in Schistosoma mansoni using antigens of Fasciola hepatica isolated by Concanavalin A affinity chromatography. Infection and Immunity, 26, 802–807.CrossRefGoogle ScholarPubMed
Hughes, D. L., Hanna, R. E. B. & Doy, T. G. (1982) Antibody response in cattle, sheep and rats to infection with γ-irradiated metacercariae of Fasciola hepatica. Research in Veterinary Science, 32, 354–358.CrossRefGoogle ScholarPubMed
Irwin, S. W. B. & Threadgold, L. T. (1970) Electron microscope studies on Fasciola hepatica: VIII. The development of the vitelline cells. Experimental Parasitology, 28, 399–411.CrossRefGoogle ScholarPubMed
Kohler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, 256, 495–497.CrossRefGoogle ScholarPubMed
Robinson, G. & Threadgold, L. T. (1975) Electron microscope studies of Fasciola hepatica. XII. The fine structure of the gastrodermis. Experimental Parasitology, 37, 20–36.CrossRefGoogle ScholarPubMed
Rowe, D. S. (1980) The role of monoclonal antibody technology in immunoparasitology. Immunology Today, 1, 30–33.CrossRefGoogle ScholarPubMed
Smith, M. A., Clegg, J. A., Snary, D. & Trejdosiewicz, A. J. (1982) Passive immunization of mice against Schistosoma mansoni with an IgM monoclonal antibody. Parasitology, 84, 83–91.CrossRefGoogle ScholarPubMed
Taylor, D. W. & Butterworth, A. E. (1982) Monoclonal antibodies against surface antigens of schistosomula of Schistosoma mansoni. Parasitology, 84, 65–82.CrossRefGoogle ScholarPubMed
Threadgold, L. T. (1967) Electron-microscope studies of Fasciola hepatica. III. Further observations on the tegument and associated structures. Parasitology, 57, 633–637.CrossRefGoogle Scholar
Threadgold, L. T. & Arme, C. (1974) Electron microscope studies of Fasciola hepatica. XI. Autophagy and parenchymal cell function. Experimental Parasitology, 35, 389–405.CrossRefGoogle ScholarPubMed
Threadgold, L. T. & Gallagher, S. S. E. (1966) Electron microscope studies of Fasciola hepatica. I. The ultrastructure and interrelationship of the parenchymal cells. Parasitology, 56, 299–304.CrossRefGoogle ScholarPubMed